Team:TU-Eindhoven/Notebook

From 2013.igem.org

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{{:Team:TU-Eindhoven/Template:Timeline | day=1-4 | month=August | year= }}
-
*...
+
''General Activities''
 +
* Regular team meetings
 +
* Looking for sponsors
 +
* Working on outreach ideas
 +
 
 +
''Wetlab''
 +
* Creating the Cultures
 +
* Retaining the DNA
 +
* Nanodrop Test
 +
 
 +
''Drylab''
 +
* Merging the stochastic simulations model for the decoy sites with the ODE model for the FNR promoter
 +
* Working on the pharmacokynetics model
 +
 
 +
''Human Practices''
 +
* Researching for facts and myths related to Synthetic Biology to be checked
 +
* Working on the fact checker web platform
 +
* Redacting the first facts to be added on our fact checker platform
 +
 
{{:Team:TU-Eindhoven/Template:TimelineEnd}}
{{:Team:TU-Eindhoven/Template:TimelineEnd}}
{{:Team:TU-Eindhoven/Template:Timeline | day=5-11 | month=August | year= }}
{{:Team:TU-Eindhoven/Template:Timeline | day=5-11 | month=August | year= }}
-
*...
+
''General Activities''
 +
* Regular team meetings
 +
* Looking for sponsors
 +
* Working on outreach ideas
 +
 
 +
''Wetlab''
 +
* Creating the Cultures
 +
* Retaining the DNA
 +
* Nanodrop Test
 +
 
 +
''Drylab''
 +
* Improving the merged model
 +
* Making an ODE model of the decoy sites
 +
* Working on the pharmacokynetics model
 +
 
 +
''Human Practices''
 +
* Working on the fact checker web platform
 +
* Preparing the instructions and example files for collaborating teams
 +
 
{{:Team:TU-Eindhoven/Template:TimelineEnd}}
{{:Team:TU-Eindhoven/Template:TimelineEnd}}
{{:Team:TU-Eindhoven/Template:Timeline | day=12-18 | month=August | year= }}
{{:Team:TU-Eindhoven/Template:Timeline | day=12-18 | month=August | year= }}
-
*...
+
''General Activities''
 +
* Regular team meetings
 +
* Looking for sponsors
 +
* Working on outreach ideas
 +
* Sharing our project with colleagues and faculty members
 +
 
 +
''Wetlab''
 +
* Digestion
 +
* Construct Digestion
 +
* Vector Digestion
 +
* PCR Purification for the Constructs
 +
* PCR Puricfication for the Vectors
 +
* Nanodrop Results
 +
* Continuing with the Purification
 +
* Vector Purification
 +
* Construct Purification
 +
* Ligation
 +
* Preparing Agar Plates
 +
* Transformation
 +
* Plating
 +
* Colony Picking and PCR
 +
* Culturing the Ligation Products
 +
 
 +
''Drylab''
 +
* Sensitivity analysis of the merged model
 +
* Estimating promotor rates
 +
* Working on the pharmacokynetics model
 +
 
 +
''Human Practices''
 +
* Working on the fact checker web platform
 +
 
{{:Team:TU-Eindhoven/Template:TimelineEnd}}
{{:Team:TU-Eindhoven/Template:TimelineEnd}}
{{:Team:TU-Eindhoven/Template:Timeline | day=19-25 | month=August | year= }}
{{:Team:TU-Eindhoven/Template:Timeline | day=19-25 | month=August | year= }}
-
*...
+
''General Activities''
 +
* Regular team meetings
 +
* Looking for sponsors
 +
* Working on outreach ideas
 +
 
 +
''Wetlab''
 +
* Colony Picking and PCR
 +
* Small Cultures and Preparation for Protein Expression
 +
* Miniprepping New Constructs
 +
* Sequencing
 +
* Trnasformation and Plating
 +
* Expressing Samples
 +
* Protein Purification
 +
* Determining Protein Concentration
 +
* SDS Analysis
 +
* Performing the Protein Expression in an Anaerobic Environment
 +
* MRI Experiments
 +
 
 +
''Drylab''
 +
* Working on the pharmacokynetics model
 +
 
 +
''Human Practices''
 +
* Fact checker platform is up and running
 +
 
{{:Team:TU-Eindhoven/Template:TimelineEnd}}
{{:Team:TU-Eindhoven/Template:TimelineEnd}}
{{:Team:TU-Eindhoven/Template:Timeline | day=26-31 | month=August | year= }}
{{:Team:TU-Eindhoven/Template:Timeline | day=26-31 | month=August | year= }}

Revision as of 19:16, 26 August 2013

In this section you can find a small description of all the work we have done so far organized by the category it belongs to.

Feb-Apr

Feb-Mar

  • Background research


Mar-Apr

  • Selection of topic
  • Further research
  • Selection of project

Apr-May

  • Further research


May

1-5 May
General Activities

  • Regular team meeting
  • Looking for sponsors
  • Month planning


Wetlab

  • Researching about the promoters
  • Researching about the possible proteins for CEST imaging


Drylab

  • Defining what needs to be modeled and what type of model is the most appropriate for it

6-12 May
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Researching and defining the killing mechanism


Drylab

  • Starting the Molecular Dynamics model for the proteins selection.

13-19 May
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Defining the needed materials
  • Defining the needed equipment


Drylab

  • Continuing the Molecular Dynamics model for the proteins selection.

20-26 May
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Locating, booking and learning how to use the necessary equipment


Drylab

  • Analyzing the results from the Molecular Dynamics model for the proteins selection and identifying the most useful proteins for CEST imaging
  • Researching and defining the needed parameters for the analysis of the decoy sites through a stochastic simulations model


Human Practices

  • Selecting a main project

27-31 May
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Locating, booking and learning how to use the necessary equipment


Drylab

  • Starting the stochastic simulations for the analysis of the decoy sites


Human Practices

  • Selecting a main project

June

3-9 June
General Activities

  • Regular team meeting
  • Looking for sponsors
  • Collaborating with Purdue iGEM team
  • Planning month activities


Wetlab

  • General construct design


Drylab

  • Continuing the stochastic simulations for the analysis of the decoy sites


Human Practices

  • Defining the structure of the fact checker project

10-16 June
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Protein design


Drylab

  • Continuing the stochastic simulations for the analysis of the decoy sites


Human Practices

  • Redacting the preliminary description and survey for collaborators of the fact checker project

17-23 June
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Final design of the constructs


Drylab

  • Continuing the stochastic simulations for the analysis of the decoy sites


Human Practices

  • Revising the preliminary description and survey for collaborators of the fact checker project

24-30 June
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Ordering the constructs
  • Working on the protocols


Drylab

  • Analyzing the results from the stochastic simulations for the analysis of the decoy sites

Human Practices

  • Researching for facts and myths related to Synthetic Biology to be checked

July

1-7 July
General Activities

  • Regular team meeting
  • Looking for sponsors
  • Planning month activities


Wetlab

  • Finalizing the protocols
  • Planning lab schedule


Drylab

  • Starting the ODE model for the analysis of the FNR promoter


Human Practices

  • Researching for facts and myths related to Synthetic Biology to be checked

8-14 July
General Activities

  • Regular team meeting
  • Looking for sponsors
  • Collaborating with Purdue iGEM team


Drylab

  • Continuing the ODE model for the analysis of the FNR promoter


Human Practices

  • Researching for facts and myths related to Synthetic Biology to be checked

15-21 July

22-28 July
General Activities

  • Regular team meetings
  • Looking for sponsors
  • Collaborating with Purdue iGEM team


Wetlab

  • Creating agar plates
  • Transforming DNA
  • Plating bacteria
  • Making LB medium
  • Creating small cultures
  • DNA retention from cultures
  • Nano-drop tests


Drylab

  • Analyzing the results from the ODE model for the analysis of the FNR promoter


Human Practices

  • Researching for facts and myths related to Synthetic Biology to be checked
  • Sending the survey to collaborators

29-31 July
General Activities

  • Regular team meetings
  • Looking for sponsors

Wetlab

  • Creating agar plates
  • Transforming DNA
  • Plating bacteria
  • Making LB medium
  • Creating small cultures
  • DNA retention from cultures
  • Nano-drop tests

Drylab

  • Merging the stochastic simulations model for the decoy sites with the ODE model for the FNR promoter


Human Practices

  • Researching for facts and myths related to Synthetic Biology to be checked

August

1-4 August
General Activities

  • Regular team meetings
  • Looking for sponsors
  • Working on outreach ideas

Wetlab

  • Creating the Cultures
  • Retaining the DNA
  • Nanodrop Test

Drylab

  • Merging the stochastic simulations model for the decoy sites with the ODE model for the FNR promoter
  • Working on the pharmacokynetics model

Human Practices

  • Researching for facts and myths related to Synthetic Biology to be checked
  • Working on the fact checker web platform
  • Redacting the first facts to be added on our fact checker platform

5-11 August
General Activities

  • Regular team meetings
  • Looking for sponsors
  • Working on outreach ideas

Wetlab

  • Creating the Cultures
  • Retaining the DNA
  • Nanodrop Test

Drylab

  • Improving the merged model
  • Making an ODE model of the decoy sites
  • Working on the pharmacokynetics model

Human Practices

  • Working on the fact checker web platform
  • Preparing the instructions and example files for collaborating teams

12-18 August
General Activities

  • Regular team meetings
  • Looking for sponsors
  • Working on outreach ideas
  • Sharing our project with colleagues and faculty members

Wetlab

  • Digestion
  • Construct Digestion
  • Vector Digestion
  • PCR Purification for the Constructs
  • PCR Puricfication for the Vectors
  • Nanodrop Results
  • Continuing with the Purification
  • Vector Purification
  • Construct Purification
  • Ligation
  • Preparing Agar Plates
  • Transformation
  • Plating
  • Colony Picking and PCR
  • Culturing the Ligation Products

Drylab

  • Sensitivity analysis of the merged model
  • Estimating promotor rates
  • Working on the pharmacokynetics model

Human Practices

  • Working on the fact checker web platform

19-25 August
General Activities

  • Regular team meetings
  • Looking for sponsors
  • Working on outreach ideas

Wetlab

  • Colony Picking and PCR
  • Small Cultures and Preparation for Protein Expression
  • Miniprepping New Constructs
  • Sequencing
  • Trnasformation and Plating
  • Expressing Samples
  • Protein Purification
  • Determining Protein Concentration
  • SDS Analysis
  • Performing the Protein Expression in an Anaerobic Environment
  • MRI Experiments

Drylab

  • Working on the pharmacokynetics model

Human Practices

  • Fact checker platform is up and running

26-31 August

  • ...

September

2-8 Sept

  • ...

9-15 Sept

  • ...

16-22 Sept

  • ...

23-29 Sept

  • ...

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