Team:Evry/Notebook/w11

From 2013.igem.org

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Top 10 <i>E. Coli</i> is trasformed with the plasmid PTK which contain &#955; Red factor.
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Top 10 <i>E. Coli</i> is transformed with the plasmid PTK which contain &#955; Red factor. We plated our bacteria on LB Agar with spectinomycin and let them grow overnight at 30°C.
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Revision as of 14:06, 27 August 2013

Iron coli project

Week 11: 26th August - 1st September

TECAN

Creation of a E. Coli ΔFur

Strain preparation

Top 10 E. Coli is transformed with the plasmid PTK which contain λ Red factor. We plated our bacteria on LB Agar with spectinomycin and let them grow overnight at 30°C.