Team:Evry/Notebook/w11
From 2013.igem.org
(Difference between revisions)
Line 18: | Line 18: | ||
<p> | <p> | ||
- | Top 10 <i>E. Coli</i> is | + | Top 10 <i>E. Coli</i> is transformed with the plasmid PTK which contain λ Red factor. We plated our bacteria on LB Agar with spectinomycin and let them grow overnight at 30°C. |
</p> | </p> | ||
Revision as of 14:06, 27 August 2013
Week 11: 26th August - 1st September
TECAN
Creation of a E. Coli ΔFur
Strain preparation
Top 10 E. Coli is transformed with the plasmid PTK which contain λ Red factor. We plated our bacteria on LB Agar with spectinomycin and let them grow overnight at 30°C.