28/08/13

(Difference between revisions)

Revision as of 13:55, 29 August 2013

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Two different sets of gel were run due to the different fragment size of the PCR product.
Smaller fragments were run on a 2% agarose gel whilst larger fragments were run on a 1% gel.

Extracting previously run DNA from gel using Thermo Scientific extraction kit and following its protocol.
Concentrations of DNA were measured by nanodrop
Sample B (Tod C gene) was not purified correctly, another PCR is needed.
Table representing the concentration and volume of DNA isolated from samples

@@ Line 13: / Line 13: @@
 </div>
-== gel electrophoresis ==
+== Gel electrophoresis ==
-* two different sets of gel were run due to the different fragment size of the PCR product.
+* Two different sets of gel were run due to the different fragment size of the PCR product.
-* smaller fragments were run on a 2% agarose gel whilst larger fragments were run on a 1% gel.
+* Smaller fragments were run on a 2% agarose gel whilst larger fragments were run on a 1% gel.
+==DNA extraction from gel==
+*Extracting previously run DNA from gel using Thermo Scientific extraction kit and following its protocol.
+*Concentrations of DNA were measured by nanodrop
+*Sample B (Tod C gene) was not purified correctly, another PCR is needed.
+*Table representing the concentration and volume of DNA isolated from samples
+{|border=1
+|Sample||Volume||Concentration ng/ul||260/280||260/230
+|-
+|A||48||19.3||2.02||0.03
+|-
+|C||50||27.7||1.88||0.04
+|-
+|D||49||26.9||1.71||0.05
+|-
+|E||50||20.5||1.98||0.03
+|}