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28/08/13
From 2013.igem.org
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*Extracting previously run DNA from gel using Thermo Scientific GeneJet gel extraction kit and following its protocol. | *Extracting previously run DNA from gel using Thermo Scientific GeneJet gel extraction kit and following its protocol. | ||
*Concentrations of DNA were measured by nanodrop | *Concentrations of DNA were measured by nanodrop | ||
| - | *Sample B (Tod C gene) was not purified correctly, another PCR is needed. | + | *Sample B (Tod C gene) was not purified correctly, another PCR is needed. (Nanodrop shows contamination) |
*Table representing the concentration and volume of DNA isolated from samples | *Table representing the concentration and volume of DNA isolated from samples | ||
| + | |||
{|border=1 | {|border=1 | ||
|Sample||Volume||Concentration ng/ul||260/280||260/230 | |Sample||Volume||Concentration ng/ul||260/280||260/230 | ||
Latest revision as of 14:01, 29 August 2013
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Gel electrophoresis
- Two different sets of gel were run due to the different fragment size of the PCR product.
- Smaller fragments were run on a 2% agarose gel whilst larger fragments were run on a 1% gel.
DNA extraction from gel
- Extracting previously run DNA from gel using Thermo Scientific GeneJet gel extraction kit and following its protocol.
- Concentrations of DNA were measured by nanodrop
- Sample B (Tod C gene) was not purified correctly, another PCR is needed. (Nanodrop shows contamination)
- Table representing the concentration and volume of DNA isolated from samples
| Sample | Volume | Concentration ng/ul | 260/280 | 260/230 |
| A | 48 | 19.3 | 2.02 | 0.03 |
| C | 50 | 27.7 | 1.88 | 0.04 |
| D | 49 | 26.9 | 1.71 | 0.05 |
| E | 50 | 20.5 | 1.98 | 0.03 |
