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Team:British Columbia/Notebook/Flavours
From 2013.igem.org
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'''Aim:''' A colony of Rhodobacter sphaeroides was inoculated in 5mL LB culture for DNA extraction | '''Aim:''' A colony of Rhodobacter sphaeroides was inoculated in 5mL LB culture for DNA extraction | ||
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=='''July 30th'''== | =='''July 30th'''== | ||
Revision as of 21:32, 31 August 2013
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Team Cinnamaldehyde & Vanillin
June 28th
Transformation of 4-coumarate-CoA Ligase (4CL) and caffeic acid-O-methyl transferase (COMT)
Experimenter: Joe
Aim: Transform biobricks 4CL ( 2013 Distribution Kit, Plate 2, Well 17P) and COMT (2013 Distribution Kit, Plate 5, Well 19D) for miniprep and later amplification through PCR
Results: Transformation done according transformation procedures. Colonies grown overnight at 37C. Only 4-CL grew overnight. No colonies were observed for COMT. Inoculated a colony of 4CL for miniprep
Rhodobacter sphaeroides Inoculation
Experimenter: Joe
Aim: A colony of Rhodobacter sphaeroides was inoculated in 5mL LB culture for DNA extraction
July 30th
Miniprep 4CL
Experimenter: Joe
Aim: Miniprep 5mL innoculation of 4CL with Qiagen Miniprep Kit
July 4th
PCR of 4-CL
Experimenter: Joe
Aim: PCR to amplify 4-CL from biobrick with biobrick VF2 and VR primers to confirm the size of 4-CL
Results: Multiple bands w
