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Team:Paris Saclay/Notebook/August/20
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=='''Lab work'''== | =='''Lab work'''== | ||
| - | ===''''''=== | + | ==='''A - Aerobic/Anaerobic regulation system'''=== |
| - | ====''''''==== | + | ===='''Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006'''==== |
| - | ===='''1 - '''==== | + | ===='''1 - Sequences analysis'''==== |
| + | Damir, XiaoJing | ||
| + | {| | ||
| + | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| + | Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006. | ||
| + | |} | ||
| + | |||
| + | ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ||
| + | |||
| + | ===='''Objective : obtaining FNR and BphR2 proteins'''==== | ||
| + | |||
| + | ===='''1 - Electrophoresis of PCR products : BphR2 Part I'''==== | ||
| + | |||
| + | Nadia | ||
| + | |||
| + | {| | ||
| + | | style="width:250px;border:1px solid black;" | [[File:Psgel2008.jpg|250px]] | ||
| + | | style="width:350px;border:1px solid black;vertical-align:top;" | | ||
| + | *Well 1 : 6µL DNA Ladder | ||
| + | *Well 2 : 40µL BphR2 Part I + 8µl of 6X loading dye | ||
| + | *Gel : 0.8% | ||
| + | |} | ||
| + | |||
| + | Expected sizes : | ||
| + | * BphR2 Part I : 178bp | ||
| + | {| | ||
| + | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| + | We obtained a frangment at the right size. We can purify it. | ||
| + | |} | ||
| + | |||
| + | ====2 - Gel purification of PCR products : BphR2 Part I ==== | ||
| + | |||
| + | Damir, Nadia | ||
| + | |||
| + | Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ] | ||
| + | |||
| + | {| | ||
| + | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| + | We lost our fragment. We will do the PCR again. | ||
| + | |} | ||
| + | |||
| + | |||
| + | {| border="1" align="center" | ||
| + | |[[Team:Paris Saclay/Notebook/August/19|<big>Previous day</big>]] | ||
| + | |||
| + | |[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]] | ||
| + | |||
| + | |[[Team:Paris Saclay/Notebook/August/21|<big>Next day</big>]] | ||
| + | |} | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Latest revision as of 01:30, 5 October 2013
Contents |
Notebook : August 20
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006
1 - Sequences analysis
Damir, XiaoJing
|
Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006. |
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins
1 - Electrophoresis of PCR products : BphR2 Part I
Nadia
|
|
Expected sizes :
- BphR2 Part I : 178bp
|
We obtained a frangment at the right size. We can purify it. |
2 - Gel purification of PCR products : BphR2 Part I
Damir, Nadia
Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
|
We lost our fragment. We will do the PCR again. |
| Previous day | Back to calendar | Next day |
