Team:TU-Delft/Safety

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<h2 align="center">Safety</h2>
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<h2 align="center">Safety</h2>
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<div style="margin-left:50px;margin-right:50px;float:left;display:inline-block;">  
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<img src="https://encrypted-tbn3.gstatic.com/images?q=tbn:ANd9GcR7t_xuhdMfKX4bRyAOH0sH9xhMAYrEOOKs_nmbiiJlZTa9n17neg" height="200px" width="200" align="left">The MRSA (Methicillin-Resistant Staphylococcus Aureus) problem is our inspiration for this project. The project would focus on killing the Staphylococcus species by antimicrobial peptides synthesized in an E.coli model organism. Careful focus on the safety aspects of the project is done to make sure that no harmful strains are let free in the lab or in the environment. Non-virulent strains of Staphylococcus Aureus or close related species to Staphylococcus species will only be used in this project. Rest other transformations are done in E. Coli strain K12. This project also looks into the safety regulations in the lab with great attention to protect any unforeseen contamination in the lab. 
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The MRSA (Methicillin-Resistant <i>Staphylococcus aureus</i>) problem is our inspiration for this project. This project will focus on killing the Staphylococcus species by antimicrobial peptides synthesized in <i>E. coli.</i>
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As we work in the lab which has only BSL-1 permit we did intense literature study to find closely related organisms of same genera other than <i>S. aureus </i> (DSMZ 6148) that can be used for our project. We used <i>S. delphini</i> (DSMZ 20771) which had some close similarities with <i>S. aureus</i> (DSMZ 6148) to test our peptides and have a prediction of their possible effect on the MRSA . The antimicrobial peptides where chosen in such a way that they have high minimum inhibitory concentration (MIC) for humans and low for <i>S. aureus</i>.
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In order prevent release of live GMO's to the environment and for a safe administration of patients , we added a kill switch that is controlled by a timer. This will allow the self destruction of the cells after the desired peptides are produced. But, there are problems of release of lippopolysaccharides (LPS) after lysis, this can be overcome by using a gram positive bacterial chassis <a href="https://2013.igem.org/Team:TU-Delft/Safety#reference">[1]</a> which does not have a problem LPS.
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<h4 align="center">Safety forms were approved on October 3, 2013 by Evan Appleton, iGEM Safety Committee.</h4>
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<a href="https://static.igem.org/mediawiki/2013/5/56/IGEM_2013_Basic_Safety_Form_signed.pdf" target="_blank">
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Safety Form 1
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<a href="https://static.igem.org/mediawiki/2013/6/63/IGEM_Biosafety_Form_Part_2_signed.pdf" target="_blank">
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Safety Form 2
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<h2 align="center">References</h2>
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<li>Volzing, K., Borrero, J., Sadowsky, M. and Kaznessis, Y. 2013. Antimicrobial Peptides Targeting Gram-negative Pathogens, Produced and Delivered by Lactic Acid Bacteria. ACS synthetic biology.
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Latest revision as of 13:34, 4 October 2013


Safety

The MRSA (Methicillin-Resistant Staphylococcus aureus) problem is our inspiration for this project. This project will focus on killing the Staphylococcus species by antimicrobial peptides synthesized in E. coli.

As we work in the lab which has only BSL-1 permit we did intense literature study to find closely related organisms of same genera other than S. aureus (DSMZ 6148) that can be used for our project. We used S. delphini (DSMZ 20771) which had some close similarities with S. aureus (DSMZ 6148) to test our peptides and have a prediction of their possible effect on the MRSA . The antimicrobial peptides where chosen in such a way that they have high minimum inhibitory concentration (MIC) for humans and low for S. aureus.

In order prevent release of live GMO's to the environment and for a safe administration of patients , we added a kill switch that is controlled by a timer. This will allow the self destruction of the cells after the desired peptides are produced. But, there are problems of release of lippopolysaccharides (LPS) after lysis, this can be overcome by using a gram positive bacterial chassis [1] which does not have a problem LPS.


Safety forms were approved on October 3, 2013 by Evan Appleton, iGEM Safety Committee.

Safety Form 1
Safety Form 2

References

  1. Volzing, K., Borrero, J., Sadowsky, M. and Kaznessis, Y. 2013. Antimicrobial Peptides Targeting Gram-negative Pathogens, Produced and Delivered by Lactic Acid Bacteria. ACS synthetic biology.
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