Team:Tuebingen/Notebook/Protocols

From 2013.igem.org

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<h3>General and <i>E. coli</i> protocols</h3>
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<ul>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/sob-medium">SOB medium</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/lb">LB medium</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/tae">TAE buffer</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/plates">LB plates</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/ladder-mix">DNA-ladder-buffer mix</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/gelelectrophoresis">Gelelectrophoresis</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/inoue">Inoue buffer</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/chemo-competentcells">Chemo-competent <i>E. coli</i> cells</li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/trafo"><i>E. coli</i> transformation</a></li> 
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<!--  <li><a href="/Team:Tuebingen/Notebook/Protocols/pgem-ligation">pGEM Ligaton - ligation for TA-cloning of PCR products</a></li> -->
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/ligation">Ligation of digested parts and vectors</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/glycerol-stocks">Glycerol stocks</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/pcr">PCR</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/3a-assembly">3A-Assembly</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/preparative-restriction">Preparative restriction digest</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/control-restriction">Control restriction digest</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/mini-prep">Plasmid Preparation</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/gelextraction">Gel Extraction</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/colony-pcr">Colony-PCR</a></li>
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</ul>
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<p><h3><i>Saccharomyces cerevisiae</i>-specific protocols</h3>
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<ul>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/ypd">YPD medium</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/sc-dropout">SC DropOut</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/onestep">ONE-STEP buffer</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/lim">Low Iron Medium (LIM)</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/yeast-trafo">Yeast transformation</a></li>
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  <li><a href="/Team:Tuebingen/Notebook/Protocols/tecanreader">Sample preparation and fluorescence measurement using the Tecan Reader</a></li>
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</ul></p>
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</div>
</div>

Latest revision as of 23:36, 15 October 2013

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