Team:TU-Delft/Planning

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Project 'Peptidor' deals with various cloning schemes into <i>E.coli</i> cells. They are made to sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and then start producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>. Several bio-bricks from the parts registry were used to clone the <i>E. coli</i>. There are many test constructs made in order to test and characterize each module. A detailed cloning strategy is as follows:  
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Project 'Peptidor' deals with various cloning schemes into <i>E.coli</i> cells. They are made to sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and then start producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>. Several bio-bricks from the parts registry were used to clone the <i>E. coli</i>. There are many test constructs made in order to test and characterize each module. Both the detailed cloning strategies related to each module and the final constructs can be viewed in the following links:
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<h4>His 6 - SUMO Peptide construct </h4> <center>
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<img src="https://static.igem.org/mediawiki/2013/4/47/His6-SUMOPeptide-construct.jpg" height=800px width=680px ></center>
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<h4>Peptide Production</h4> <center>
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<img src="https://static.igem.org/mediawiki/2013/3/30/Peptide-Production.jpg" height=600px width=680px ></center>
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<li><a href="https://2013.igem.org/Team:TU-Delft/PeptideProduction" style="text-decoration: none"><font color="#0080FF" size="3">Peptide Production Cloning Schemes</font></a></li>
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<li><a href="https://2013.igem.org/Team:TU-Delft/KillSwitchPlanning" style="text-decoration: none"><font color="#0080FF" size="3">Kill Switch Cloning Schemes</font></a></li>
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<li><a href="https://2013.igem.org/Team:TU-Delft/Sensing Device" style="text-decoration: none"><font color="#0080FF" size="3">Sensing Device Cloning Schemes</font></a></li>
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<li><a href="https://2013.igem.org/Team:TU-Delft/FinalConstructs" style="text-decoration: none"><font color="#0080FF" size="3">Final Constructs</font></a></li>
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<li><a href="https://2013.igem.org/Team:TU-Delft/All" style="text-decoration: none"><font color="#0080FF" size="3">All Cloning Schemes</font></a> </li>
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<h4>AIP test Construct </h4><center>
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<img src="https://static.igem.org/mediawiki/2013/d/d4/AIP-test-constructs.jpg" height=950px width=700px ></center>
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<h4> </h4> <center>
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<img src="  " height=950px width=680px ></center>
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Latest revision as of 14:57, 18 September 2013


Planning

Project 'Peptidor' deals with various cloning schemes into E.coli cells. They are made to sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and then start producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Several bio-bricks from the parts registry were used to clone the E. coli. There are many test constructs made in order to test and characterize each module. Both the detailed cloning strategies related to each module and the final constructs can be viewed in the following links:
















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