Team:Paris Saclay/Notebook/August/20

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=='''Lab work'''==
=='''Lab work'''==
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==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===
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==='''A - Aerobic/Anaerobic regulation system'''===
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===='''Objective : obtaining FNR and BphR2 proteins'''====
+
===='''Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006'''====
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===='''1 - PCR of BphR2 Part I'''====
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===='''1 - Sequences analysis'''====
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Damir
+
Damir, XiaoJing
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Used quantities :  
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{|
-
*
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| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
 +
Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006.
 +
|}
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PCR Program :
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==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===
-
===='''2 - Electrophoresis of PCR products :  BphR2 Part I'''====
+
===='''Objective : obtaining FNR and BphR2 proteins'''====
 +
 
 +
===='''1 - Electrophoresis of PCR products :  BphR2 Part I'''====
Nadia
Nadia
{|
{|
-
| style="width:250px;border:1px solid black;" | [[]]
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| style="width:250px;border:1px solid black;" | [[File:Psgel2008.jpg|250px]]
| style="width:350px;border:1px solid black;vertical-align:top;" |
| style="width:350px;border:1px solid black;vertical-align:top;" |
*Well 1 : 6µL DNA Ladder
*Well 1 : 6µL DNA Ladder
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*Well 2 : 40µL BphR2 PartI+8µl of 6X loading dye
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*Well 2 : 40µL BphR2 Part I + 8µl of 6X loading dye
*Gel : 0.8%
*Gel : 0.8%
|}  
|}  
 +
Expected sizes :
 +
* BphR2 Part I : 178bp
{|
{|
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
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We obtain a frangment at the right size. We can purify it.
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We obtained a frangment at the right size. We can purify it.
-
|}  
+
|}
====2 - Gel purification of PCR products : BphR2 Part I ====
====2 - Gel purification of PCR products : BphR2 Part I ====
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Damir, Nadia
Damir, Nadia
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Protocol : [[Team:Paris_Saclay/Protocols/Gel purification|Gel purification]]
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Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
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+
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Nanodrop :
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* BphR2 Part I: .........
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  {|
  {|
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
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CONCLUSION ON A TOUS PERDU D'OU ON RECOMMENCE ENSUITE ??????
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We lost our fragment. We will do the PCR again.
|}
|}
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{| border="1" align="center"
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|[[Team:Paris Saclay/Notebook/August/19|<big>Previous day</big>]]
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|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
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|[[Team:Paris Saclay/Notebook/August/21|<big>Next day</big>]]
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|}
{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Latest revision as of 01:30, 5 October 2013

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Contents

Notebook : August 20

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006

1 - Sequences analysis

Damir, XiaoJing

Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006.

A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Objective : obtaining FNR and BphR2 proteins

1 - Electrophoresis of PCR products : BphR2 Part I

Nadia

Psgel2008.jpg
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 40µL BphR2 Part I + 8µl of 6X loading dye
  • Gel : 0.8%

Expected sizes :

  • BphR2 Part I : 178bp

We obtained a frangment at the right size. We can purify it.

2 - Gel purification of PCR products : BphR2 Part I

Damir, Nadia

Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]

We lost our fragment. We will do the PCR again.


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