"
Team:Paris Saclay/Notebook/August/20
From 2013.igem.org
(Difference between revisions)
(→1 - Sequences analysis) |
|||
| (13 intermediate revisions not shown) | |||
| Line 7: | Line 7: | ||
==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006'''==== |
===='''1 - Sequences analysis'''==== | ===='''1 - Sequences analysis'''==== | ||
| Line 13: | Line 13: | ||
Damir, XiaoJing | Damir, XiaoJing | ||
| - | .. | + | {| |
| + | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| + | Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006. | ||
| + | |} | ||
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ||
| Line 19: | Line 22: | ||
===='''Objective : obtaining FNR and BphR2 proteins'''==== | ===='''Objective : obtaining FNR and BphR2 proteins'''==== | ||
| - | ===='''1 | + | ===='''1 - Electrophoresis of PCR products : BphR2 Part I'''==== |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
Nadia | Nadia | ||
{| | {| | ||
| - | | style="width:250px;border:1px solid black;" | [[]] | + | | style="width:250px;border:1px solid black;" | [[File:Psgel2008.jpg|250px]] |
| style="width:350px;border:1px solid black;vertical-align:top;" | | | style="width:350px;border:1px solid black;vertical-align:top;" | | ||
*Well 1 : 6µL DNA Ladder | *Well 1 : 6µL DNA Ladder | ||
| - | *Well 2 : 40µL BphR2 | + | *Well 2 : 40µL BphR2 Part I + 8µl of 6X loading dye |
*Gel : 0.8% | *Gel : 0.8% | ||
|} | |} | ||
| + | Expected sizes : | ||
| + | * BphR2 Part I : 178bp | ||
{| | {| | ||
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| - | We | + | We obtained a frangment at the right size. We can purify it. |
| - | |} | + | |} |
====2 - Gel purification of PCR products : BphR2 Part I ==== | ====2 - Gel purification of PCR products : BphR2 Part I ==== | ||
| Line 49: | Line 45: | ||
Damir, Nadia | Damir, Nadia | ||
| - | Protocol : [ | + | Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ] |
| - | + | ||
| - | + | ||
| - | + | ||
{| | {| | ||
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| - | + | We lost our fragment. We will do the PCR again. | |
|} | |} | ||
| + | |||
| + | {| border="1" align="center" | ||
| + | |[[Team:Paris Saclay/Notebook/August/19|<big>Previous day</big>]] | ||
| + | |||
| + | |[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]] | ||
| + | |||
| + | |[[Team:Paris Saclay/Notebook/August/21|<big>Next day</big>]] | ||
| + | |} | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Latest revision as of 01:30, 5 October 2013
Contents |
Notebook : August 20
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006
1 - Sequences analysis
Damir, XiaoJing
|
Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006. |
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins
1 - Electrophoresis of PCR products : BphR2 Part I
Nadia
|
|
Expected sizes :
- BphR2 Part I : 178bp
|
We obtained a frangment at the right size. We can purify it. |
2 - Gel purification of PCR products : BphR2 Part I
Damir, Nadia
Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
|
We lost our fragment. We will do the PCR again. |
| Previous day | Back to calendar | Next day |
