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18/09/13
From 2013.igem.org
(Difference between revisions)
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| + | <div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;"> | ||
| + | {| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center" | ||
| + | !align="center"|[[Team:Leicester|Home]] | ||
| + | !align="center"|[[Team:Leicester/Team|Team]] | ||
| + | !align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile] | ||
| + | !align="center"|[[Team:Leicester/Project|Project]] | ||
| + | !align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]] | ||
| + | !align="center"|[[Team:Leicester/Modeling|Modeling]] | ||
| + | !align="center"|[[Team:Leicester/Notebook|Notebook]] | ||
| + | !align="center"|[[Team:Leicester/Safety|Safety]] | ||
| + | !align="center"|[[Team:Leicester/Attributions|Attributions]] | ||
| + | |} | ||
| + | </div> | ||
| + | |||
| + | |||
==Making and running an agarose gel== | ==Making and running an agarose gel== | ||
*Making a 3% agarose gel to run Tod PCR fusion genes from 17/09 | *Making a 3% agarose gel to run Tod PCR fusion genes from 17/09 | ||
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*Digested samples were taken from the digestions the Tod genes from previous day | *Digested samples were taken from the digestions the Tod genes from previous day | ||
*100ng of both samples were run against a 100kb ladder | *100ng of both samples were run against a 100kb ladder | ||
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| + | [[File:morninggel_180913.jpg]] | ||
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| + | Rows are as follow:100bp ladder, undigested x, digested x, undigested b, digested b, undigested f, digested f | ||
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| + | ==Plating out TOD operon bacteria== | ||
| + | *For selection of individual colonies | ||
Latest revision as of 09:59, 20 September 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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Making and running an agarose gel
- Making a 3% agarose gel to run Tod PCR fusion genes from 17/09
- This is to determine that the digestion works
- Running the gel with digested and undigested samples to observe the difference
- Digested samples were taken from the digestions the Tod genes from previous day
- 100ng of both samples were run against a 100kb ladder
Rows are as follow:100bp ladder, undigested x, digested x, undigested b, digested b, undigested f, digested f
Plating out TOD operon bacteria
- For selection of individual colonies
