Team:NYMU-Taipei

From 2013.igem.org

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Latest revision as of 02:41, 29 October 2013

National Yang Ming University
Taipei, Taiwan

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Overview of 2013 NYMU iGEM Team Project

To save bees from Nosema ceranae infection, the culprit of colony collapse disorder (CCD), NYMU-Taipei iGEM team has created the so-called Bee. coli from Escherichia coli MG1655, which naturally resides in bees. The Bee. coli is designed to work successively as follows: (1) Bee. coli continuously secretes mannosidase to inhibit the sprouting of N. ceranae spores. (2) if the bee is infected with N. ceranae, the fungus-killing-circuit with a positive feedback design will be turned on to wipe out N. ceranae. (3) if these designer weapons should fail to conquer N. ceranae, a bee-suicide-operon will be activated to kill the infected bees but save their companions.

Besides, a light-inducible lysis system is created to ensure our Bee. coil only lives inside of the bee. In addition, we have used encapsulation as the method to send our Bee. coli into the bee. Since the capsule will only dissolve in a bee’s gut, our Bee. coli will not wantonly spread out.

Our 2013 iGEM Awards

☆ Finalist

☆ 1st runner up

☆ Best Presentation

☆ Best Parts Collection

☆ Most Improved Registry Part

☆ Best New BioBrick Part or Device, Engineered

List of Our Experimental Accomplishments

✓ 23 new BioBricks to part registry

✓ A way to transport genetic modified E. coli

✓ A light-induced lysis system for safety

✓ The improvement of BioBrick Part AhpCp

✓ Modeling standard for prescription of engineered E. coli

✓ An inducible delay positive feedback circuit

✓ Help NTU-Taida iGEM team characterize their BioBrick devices

✓ Video documentations of performing experimental procedures

✓ Cooperation with beekeepers

✓ Primers for identifying Nosema

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-{{:Team:NYMU-Taipei/Header}}
+{{:Team:NYMU-Taipei/Frontpage}}
-==Overview==
+==Overview of 2013 NYMU iGEM Team Project==
-===Saving the Bee from CCD===
-In hope of protecting the eco-system, food supply and agricultural economy, the goal of our project is to solve the Colony Collapse Disorder (CCD), a severe disease that causes bees to dramatically decline or disappear. Since the culprit of CCD is a microsporidian called ''Nosema ceranae'', we created Bee. coli to strengthen bees’ immune system and further wipe ''N. ceranae'' out.
-The dormant stage of ''N. ceranae'' is a long-lived spore which is small enough to be ingested by bees through food or water. When ''N. ceranae'' travels to the bees’ midgut, it will germinate a polar filament to reach the epithelial cells. Midgut cells are then dominated by ''N. ceranae'' and thus bee’s ability to gain nutrition is decreased. In addition, some spores will come out in feces, leading to feces-oral or oral-oral infection in the beehive. ''N. ceranae'' is fatal to bees; however, so far there has been no efficient way to stop this pathogen. This year, we endow Bee. coli with multiple functions to resist the invasion of ''N. ceranae'', hoping to cure CCD and prevent colonial infection.
+To save bees from ''Nosema ceranae'' infection, the culprit of colony collapse disorder (CCD), NYMU-Taipei iGEM team has  created the so-called ''Bee. coli'' from ''Escherichia coli'' MG1655, which naturally resides in bees. The ''Bee. coli'' is designed to work successively as follows: (1) ''Bee. coli'' continuously secretes mannosidase to inhibit the sprouting of N. ceranae spores. (2) if the bee is infected with ''N. ceranae'', the fungus-killing-circuit with a positive feedback design will be turned on to wipe out ''N. ceranae''. (3) if these designer weapons should fail to conquer ''N. ceranae'', a bee-suicide-operon will be activated to kill the infected bees but save their companions.
-Bee. coli’s functions are designed to work sequentially. Before ''N. ceranae'' approaches the midgut cells, Bee. coli will secrete mannosidase to inhibit ''N. ceranae'' from growing a polar filament. The epithelial cells will secrete reactive oxygen species (ROS) once being attacked by ''N. ceranae''. ROS will activate promoters containing OxyR binding site in Bee. coli, serving as a signal of ''N. ceranae'' invasion, eventually resulting in Bee. coli producing substances such as defensin and abaecin to kill ''N. ceranae'' while still being safe to bees and Bee. coli itself. Moreover, if the methods above unfortunately fail, Bee. coli will secrete ethanol to kill the single bee, that is to say, to sacrifice few infected bees in order to protect the healthy bees.
+Besides, a light-inducible lysis system is created to ensure our ''Bee. coil'' only lives inside of the bee. In addition, we have used encapsulation as the method to send our ''Bee. coli'' into the bee. Since the capsule will only dissolve in a bee’s gut, our ''Bee. coli'' will not wantonly spread out.
-Concerning safety, Bee. coli is designed to commit suicide if it escapes from midgut. Once Bee. coli senses that the pH of the environment is different from that of the midgut, Bee. coli will produce a lysis protein and kill itself. Last but not least, to make Bee. coli more practical for survival, we chose to use ''E. coli'' K-12 MG1655, a bacteria naturally occurring in honeybees' gut. We then use encapsulation to transport Bee. coli into the bee’s midgut. The beekeepers can add the Bee. coli capsules into sugar-water which are then consumed by bees. That way, Bee. coli can come into reality and solve the CCD problem.
+[[File:NYMU_project_overview.png|900px|center|link=https://2013.igem.org/Team:NYMU-Taipei/Project/Overview]]
+==[https://2013.igem.org/Jamborees-Asia Our 2013 iGEM Awards]==
+[[File:i'm the winner.png|right|200px]]
+===☆ Finalist===
+===☆ 1st runner up===
+===☆ Best Presentation===
+===☆ Best Parts Collection===
+===☆ Most Improved Registry Part===
+===☆ Best New BioBrick Part or Device, Engineered===
+[[File:NYMU_we're_the_winnner.png|800px|center]]
+==List of Our Experimental Accomplishments==
+[https://2013.igem.org/Team:NYMU-Taipei/Experiment/Wet_Lab ✓ 23 new BioBricks to part registry]
+[https://2013.igem.org/Team:NYMU-Taipei/Project/Enter ✓ A way to transport genetic modified ''E. coli'']
+[https://2013.igem.org/Team:NYMU-Taipei/Project/safe ✓ A light-induced lysis system for safety]
+[https://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor ✓ The improvement of BioBrick Part AhpCp]
+[https://2013.igem.org/Team:NYMU-Taipei/Modeling/Overview ✓ Modeling standard for prescription of engineered ''E. coli'']
+[https://2013.igem.org/Team:NYMU-Taipei/Modeling/Ethanol ✓ An inducible delay positive feedback circuit]
+[https://2013.igem.org/Team:NYMU-Taipei/HumanPractice/HumCollab ✓ Help NTU-Taida iGEM team characterize their BioBrick devices]
+[https://2013.igem.org/Team:NYMU-Taipei/HumanPractice/HumFlows ✓ Video documentations of performing experimental procedures]
+[https://2013.igem.org/Team:NYMU-Taipei/HumanPractice/HumPracticalApp ✓ Cooperation with beekeepers]
+[https://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Id-Nosema ✓ Primers for identifying Nosema]
+{{:Team:NYMU-Taipei/Frontpage/Footer}}