20/09/13

From 2013.igem.org

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(Created page with "==Miniprep from overnight broths== *DNA is isolated using Omega Miniprep kit and its protocol is followed ==Nanodropping the isolated DNA== {|border=1 |Sample||Volume ul||Concen...")
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<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;">
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{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
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!align="center"|[[Team:Leicester|Home]]
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!align="center"|[[Team:Leicester/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
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!align="center"|[[Team:Leicester/Project|Project]]
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!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Leicester/Modeling|Modeling]]
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!align="center"|[[Team:Leicester/Notebook|Notebook]]
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!align="center"|[[Team:Leicester/Safety|Safety]]
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!align="center"|[[Team:Leicester/Attributions|Attributions]]
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|}
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</div>
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==Miniprep from overnight broths==
==Miniprep from overnight broths==
*DNA is isolated using Omega Miniprep kit and its protocol is followed
*DNA is isolated using Omega Miniprep kit and its protocol is followed
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|Sample||Volume ul||Concentration ng/ul||260/280||260/230
|Sample||Volume ul||Concentration ng/ul||260/280||260/230
|-
|-
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|B5||
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|X12||90||13.5||1.86||1.38
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|-
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|X3||43||13.6||2.12||1.34
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|-
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|X5||45||16||2.08||1.64
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|-
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|X6||44||19.1||2.04||1.59
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|-
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|B4||93||11.1||1.73||1.28
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|-
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|B5||87||9.5||1.99||1.34
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|-
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|B9||43||13.1||2.14||1.43
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|-
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|B12||43||14.9||1.99||1.23
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|-
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|F1||92||17.6||1.91||1.15
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|-
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|F8||93||17.6||1.85||1.70
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|-
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|F10||91||12.4||1.99||1.30
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|-
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|F12||41||18.5||2.06||1.71
|}
|}
==Double digest of isolated DNA==
==Double digest of isolated DNA==
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*Digesting with EcorI-HF and PstI-HF to see if insert has been incorporated into DNA
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*Digesting with EcorI-HF/PstI-HF and XbaI/PstI to see if DNA incorporated insert has the biobrick prefix and suffix present.
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*Gel to be run in the 23/09/13
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==Running on a 1.5% gel==
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*Gel pre-made on 18/09/13
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*Running at 100V for 1.5 hours
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Latest revision as of 15:20, 23 September 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


Miniprep from overnight broths

  • DNA is isolated using Omega Miniprep kit and its protocol is followed

Nanodropping the isolated DNA

SampleVolume ulConcentration ng/ul260/280260/230
X129013.51.861.38
X34313.62.121.34
X545162.081.64
X64419.12.041.59
B49311.11.731.28
B5879.51.991.34
B94313.12.141.43
B124314.91.991.23
F19217.61.911.15
F89317.61.851.70
F109112.41.991.30
F124118.52.061.71

Double digest of isolated DNA

  • Digesting with EcorI-HF/PstI-HF and XbaI/PstI to see if DNA incorporated insert has the biobrick prefix and suffix present.
  • Gel to be run in the 23/09/13
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