Team:USTC CHINA

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        <li class="active"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project" target="_blank">Project</a>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview" target="_blank">Overview</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails" target="_blank">Project Details</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results" target="_blank">Results</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook" target="_blank">Notebook</a>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline" target="_blank">Timeline</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></li>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols" target="_blank">Protocols</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/" target="_blank">Modeling</a>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ReporterSystem" target="_blank">Reporter System</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></li>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture" target="_blank">B.Subtilis Culture</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs of Immune Experiments</a></li>
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              <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice" target="_blank">Human Practice</a>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" target="_blank">Communication</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" >Communication</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team" target="_blank">Team</a>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team/Members" target="_blank">Members</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Members</a></li>
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                        <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA" target="_blank">Profile</a></li>
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                  <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></li>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team/Attribute" target="_blank">Attribute</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></li>
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                  <li><a href="https://igem.org/2013_Judging_Form?id=1074#iGEM_Medals">Achievements</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
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<h1>In Situ Transdermal Vaccine</h1></div>
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        <h1>In Situ <span>T</span>ransdermal <span>Vaccine</span></h1>
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<p>Fresh Expression, No Purification<br />Standardization, Block-based design<br />Easy-transportation, Low Storage External Costs<br />
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Almost No Demand For Medical Conditions And Proferssionals<br /></p>
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<p></p><p><a class="button-home" href="https://2013.igem.org/Team:USTC_CHINA/Project"><span>Project Details</span></a></p></div>
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        <p>2013, We Redefined Vaccine<br />A Revolutionary Transdermal Vaccine<br />Safe, Reliable, Needle-free and Available for Everyone<br />
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<h1>Why We Begin</h1>
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<h1>Motivation</h1>
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   we hope to provide a needle-free and non-skin-damage vaccination,
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   We hope to develop a fresh-expression transdermal vaccination.
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   <br />we hope everyone, even in the least developed area, could enjoy the benefit of medical progress.<br />
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   <br />We hope everyone in this world could equally enjoy the benefit of medical progress.<br />
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   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Background">more about project background</a>
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   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">more</a>
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<h1>How We Design</h1>
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<h1>Design of Project</h1>
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   With the support of <span>TD-1</span>, a special polypeptide, which can greatly facilitate macromolecule transdermal delivery through intact skin, we construct three different fuctional engineered bacillus subtilis; besides, we design a kill switch in the fouth type B.subtilis to ensure biosafety.</div>
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   With the support of <span><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background#" target="_blank">TD-1</a></span>, a special polypeptide which can greatly facilitate macromolecule transdermal delivery across intact skin, we constructed three different fuctional engineered <i>Bacillus subtilis</i>; besides, we designed a reporter system, including a kill switch, in the fouth type <i>B.subtilis</i> to improve usability and ensure biosafety.</div>
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   <span>ANTIGEN</span><br />
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As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will share a great percentage of<br /> the total bacteria in our "band-aid".
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As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will make up great percentage of<br />in the total bacteria in our "band-aid".
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   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>
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   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
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   <span>LTB</span><br />
   <span>LTB</span><br />
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As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a wild using adjuvant for most of exsiting injected vaccine.
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As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a widely used adjuvant for most of exsiting injected vaccine.
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   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>
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   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
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   <span>KNFα</span><br />
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   <span>TNFα</span><br />
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It's worth mentioning that to improve the effects of <br />traditional vaccine, as another kind of our TD1-adjuvant expresser, KNFα bear the weight of our hope to increase the sensitivity of our immune system.
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TNFα is a chemotactic factor recruiting Langerhans Cells, the antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for collecting LC and triggering more intense immune responses.
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   <span>REPORTER</span><br />
   <span>REPORTER</span><br />
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Reporter, which notifies users whether the status of vaccine patch is all right and when they can stick the patches to arms .  Further more killing switch is existed in them as a biosafety guard.
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Reporter notifies users the status of vaccine patch and when to stick.  Furthermore, it contains killing switch as a biosafety guard.
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<h1>What We've Achived</h1>
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<h1>Experimental Measurement</h1>
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  <span>Molecules part</span><br />
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  <span>Construction of Vectors</span><br />
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We took E.coli and the PET vector as the positive control and realized our ideas in the B.subtilis expression system based on PHT43 shuttle vector.<br />
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We took <i>E.coli</i> and the pET vector as the positive control and realized our ideas in the <div class="oblique">B.subtilis</div> expression system based on pHT43 shuttle vector.<br />
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   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more about project results</a>
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   <span>Protein part</span><br />
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   <span>Tests on fusion protein</span><br />
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   Before we achieve fresh expression, we need first use purified protein to obtain exact data to verify our assumption.<br />
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   To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verify our design.<br />
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   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Results">more about project results</a>
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   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a>
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   <span>Mice experiments</span><br />
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   <span>Immunogenicity experiments</span><br />
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   The most obvious character of our 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also tried mice experiments, which could be divided into transdermal experiments in vitro and animate mice experiments<br />
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   The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments<br />
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   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more about project results</a>
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<div id="modle-bar">
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<h1>Modleing</h1>
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<h1>Modeling</h1>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">Suicide mechanism of the reporter system</a></h2>
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<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture"><img src="images/model1.png" width="310" height="180" /></a>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Suicide mechanism of the reporter system</a></h2>
 +
<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch"><img src="https://static.igem.org/mediawiki/2013/4/48/2013ustc-china_Model1.png" width="310" height="160" /></a>
<br />
<br />
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ReporterSystem">The optimal culture conditions of Bacillus subtilis</a></h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">The optimal culture conditions of Bacillus subtilis</a></h2>
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<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ReporterSystem"><img src="images/model2.png" width="310" height="264" /></a>
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<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture"><img src="https://static.igem.org/mediawiki/2013/1/10/2013ustc-china_Model2.png" width="310" height="234" /></a>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/MiceModeling" target="_blank">
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">
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Mice Modeling</a></h2>
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Designs of Immune Experiments</a></h2>
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<h1>Human Practice</h1>
<h1>Human Practice</h1>
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Communication</a></h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" target="_blank">
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication">
Activity</a></h2>
Activity</a></h2>
</a>
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     <div class="sponsor" id="db">
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       <p align="center">Deutsche Bank, China</p>
       <p align="center">Deutsche Bank, China</p>
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         <div align="center"><a href="https://china.db.com/index_e.html" target="_blank"><img src="images/USTC-Software-images-db-logo.png" alt="Deutsche Bank, China" height="65px" align="absmiddle" width="280px"></a>
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         <div align="center"><a href="https://china.db.com/index_e.html" target="_blank"><img src="https://static.igem.org/mediawiki/2013/a/a7/2013ustc-china_Db-logo.png" alt="Deutsche Bank, China" height="65px" align="absmiddle" width="280px"></a>
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         <p align="center">School of Life Sciences, USTC</p>
         <p align="center">School of Life Sciences, USTC</p>
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         <div align="center"><a href="http://en.biox.ustc.edu.cn/" target="_blank"><img src="images/USTC-Software-images-life-logo.png" alt="School of Life Sciences, USTC" height="65px" align="absmiddle" width="280px"></a>
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         <div align="center"><a href="http://en.biox.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/9/98/2013ustc-china_Life-logo.png" alt="School of Life Sciences, USTC" height="65px" align="absmiddle" width="280px"></a>
         </div>
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       <p align="center">USTC<span style="color:#fcc644">IF</span></p>
       <p align="center">USTC<span style="color:#fcc644">IF</span></p>
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         <div align="center"><a href="http://www.ustcif.org/" target="_blank"><img src="images/USTC-Software-images-ustcif-logo.png" height="65px" align="absmiddle" width="280px"></a>
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         <div align="center"><a href="http://www.ustcif.org/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/3/3c/2013ustc-china_Ustcif-logo.png" height="65px" align="absmiddle" width="280px"></a>
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         <p align="center">School of the Gifted Young, USTC</p>
         <p align="center">School of the Gifted Young, USTC</p>
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         <div align="center"><a href="http://en.scgy.ustc.edu.cn/" target="_blank"><img src="images/Gifted_young.jpg" alt="School of the Gifted Young, USTC" height="65px" align="absmiddle" width="280px"></a>
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         <div align="center"><a href="http://en.scgy.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/c/c1/2013ustc-china_Gifted_young.jpg" height="65px" align="absmiddle" width="280px"></a>
         </div>
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     <div class="sponsor">
         <p align="center">School of physics, USTC</p>
         <p align="center">School of physics, USTC</p>
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         <div align="center"><a href="http://en.physics.ustc.edu.cn/" target="_blank"><img src="images/USTC-Software-images-physics-logo.png" height="65px" align="absmiddle" width="280px"></a>
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         <div align="center"><a href="http://en.physics.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/6/68/2013ustc-china_Physics-logo.png" height="65px" align="absmiddle" width="280px"></a>
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     <div class="sponsor">
         <p align="center">Teaching Affairs Office of USTC</p>
         <p align="center">Teaching Affairs Office of USTC</p>
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         <div align="center"><a href="http://www.teach.ustc.edu.cn/" target="_blank"><img src="images/USTC-Software-images-teaching-logo.png" alt="Teaching Affairs Office Of USTC" style="background-color:white" height="65px" align="absmiddle" width="280px"></a>
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         <div align="center"><a href="http://www.teach.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/f/f9/2013ustc-china_Teaching-logo.png" alt="Teaching Affairs Office Of USTC" style="background-color:white" height="65px" align="absmiddle" width="280px"></a>
         </div>
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Latest revision as of 03:28, 29 October 2013

USTC_China

In Situ Transdermal Vaccine

2013, We Redefined Vaccine
A Revolutionary Transdermal Vaccine
Safe, Reliable, Needle-free and Available for Everyone

Project

Motivation

We hope to develop a fresh-expression transdermal vaccination.
We hope everyone in this world could equally enjoy the benefit of medical progress.
more

Design of Project

With the support of TD-1, a special polypeptide which can greatly facilitate macromolecule transdermal delivery across intact skin, we constructed three different fuctional engineered Bacillus subtilis; besides, we designed a reporter system, including a kill switch, in the fouth type B.subtilis to improve usability and ensure biosafety.
ANTIGEN
As the core of our in-situ system,
TD1-antigen expresser is designed
and will make up great percentage of
in the total bacteria in our "band-aid".
more
LTB
As one kind of our adjuvant expresser,
TD1-LTB is used to enhance the antigenicity.
For LTB has so many advantages, it's a widely used adjuvant for most of exsiting injected vaccine.
more
TNFα
TNFα is a chemotactic factor recruiting Langerhans Cells, the antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for collecting LC and triggering more intense immune responses.
more
REPORTER
Reporter notifies users the status of vaccine patch and when to stick. Furthermore, it contains killing switch as a biosafety guard.
more

Experimental Measurement

Construction of Vectors
We took E.coli and the pET vector as the positive control and realized our ideas in the
B.subtilis
expression system based on pHT43 shuttle vector.
more
Tests on fusion protein
To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verify our design.
more
Immunogenicity experiments
The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments
more

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