Team:USTC CHINA/Project/Overview

From 2013.igem.org

(Difference between revisions)
(Created page with "{{USTC-China/hidden}} <html> <head> <link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:USTC_CHINA/main.css?action=raw&ctype=text/css" /> <link rel="stylesheet...")
 
(57 intermediate revisions not shown)
Line 4: Line 4:
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:USTC_CHINA/main.css?action=raw&ctype=text/css" />
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:USTC_CHINA/main.css?action=raw&ctype=text/css" />
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:USTC_CHINA/pro.css?action=raw&ctype=text/css" />
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:USTC_CHINA/pro.css?action=raw&ctype=text/css" />
 +
<style type="text/css">
 +
</style>
</head>
</head>
-
<body>
+
<body background="https://static.igem.org/mediawiki/2013/6/62/2013ustc-china_Light_grey_bg.png">
<div class="bar" align="center">
<div class="bar" align="center">
     <div class="container" align="left">
     <div class="container" align="left">
-
        <div id="tlogo"><img src="images/T-VACCINE.png" width="100%" height="123" />
+
<div id="igemlogo"><a href="https://2013.igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2013/2/26/2013ustcigem_IGEM_basic_Logo.png" alt="igem home page" width="50" height="40" /></a></div>
-
        </div>
+
 
-
       
+
<ul id="nav">
<ul id="nav">
-
                <li><a href="https://2013.igem.org/Team:USTC_CHINA" target="_blank">Home</a></li>
+
        <li><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a></li>
-
                <li class="active"><a href="https://2013.igem.org/Team:USTC_CHINA/Project" target="_blank">Project</a>
+
        <li class="active"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a>
-
                    <ul class="subs">
+
            <ul class="subs">
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview" target="_blank">Overview</a></li>
+
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails" target="_blank">Project Details</a></li>
+
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results" target="_blank">Results</a></li>
+
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
-
                    </ul>
+
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
-
                </li>
+
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
-
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook" target="_blank">Notebook</a>
+
            </ul>
-
                    <ul class="subs">
+
        </li>
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline" target="_blank">Timeline</a></li>
+
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a>
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols" target="_blank">Protocols</a></li>
+
              <ul class="subs">
-
                    </ul>
+
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></li>
-
                </li>
+
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></li>
-
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/" target="_blank">Modeling</a>
+
              </ul>
-
                    <ul class="subs">
+
        </li>
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ReporterSystem" target="_blank">Reporter System</a></li>
+
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a>
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture" target="_blank">B.Subtilis Culture</a></li>
+
              <ul class="subs">
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/MiceModeling" target="_blank">Mice Modeling</a></li>
+
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></li>
-
                    </ul>
+
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></li>
-
              </li>
+
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs of Immune Experiments</a></li>
-
              <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice" target="_blank">Human Practice</a>
+
              </ul>
-
                    <ul class="subs">
+
        </li>
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" target="_blank">Communication</a></li>
+
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice">Human Practice</a>
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Activity" target="_blank">Activity</a></li>
+
              <ul class="subs">
-
                    </ul>
+
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" >Communication</a></li>
-
              </li>
+
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Activity">Activity</a></li>
-
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team" target="_blank">Team</a>
+
              </ul>
-
                <ul class="subs">
+
        </li>
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team/Members" target="_blank">Members</a></li>
+
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Team</a>
-
                        <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA" target="_blank">Profile</a></li>
+
              <ul class="subs">
-
                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team/Attribute" target="_blank">Attribute</a></li>
+
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Members</a></li>
-
                  </ul>
+
                  <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></li>
-
              </li>
+
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></li>
-
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
+
                  <li><a href="https://igem.org/2013_Judging_Form?id=1074#iGEM_Medals">Achievements</a></li>
-
            </ul>
+
              </ul>
 +
        </li>
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
 +
    </ul>
 +
        <div id="tlogo"><img src="https://static.igem.org/mediawiki/2013/f/f8/2013ustc-china_T-VACCINE.png" width="100%" height="123" />
 +
        </div>
         </div>
         </div>
         </div>
         </div>
Line 53: Line 59:
<div class="content" align="center">
<div class="content" align="center">
<div class="conbar1">
<div class="conbar1">
-
<div id="breadcrumb"><a href="">Home</a> &gt; <a href="">project</a> &gt; <a href="">overview</a></div></div>
+
<div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div></div>
<div class="conbar2">
<div class="conbar2">
<div class="leftbar" align="left">
<div class="leftbar" align="left">
-
         <div id="overview-bar" class="bassic-bar">
+
         <div id="overview-bar" class="basic-bar">
-
         <div class="home3col-ribbon"></div>
+
         <h1>Overview</h1>
-
        <div id="tag" class="title"><h1>overview</h1></div>
+
         <p align="justify">In our world, billions of people are suffering from contagions while only parts of contagions can be effectively prevented by existing vaccines. The disadvantages of traditional vaccines, like  being produced and purified with strict requirements on temperature, have limited their application, especially in developing countries. </p>
-
         <p>T-VACCINE——A vaccine that can initiate immune response by penetrating the skin with the aid of transdermal peptide. From now on, injections are simply history! Based on the theory of user-friendly, a special group of engineering bacteria which produce T-VACCINE is used to create a brand-new "band-aid" that can serve as a guardian of our health .<br />
+
<div align="center"><img src="https://static.igem.org/mediawiki/igem.org/e/ec/2013ustc-china_traditional_vaccine_transport.jpg" width="400" height="300" />
-
We have found a kind of transdermal peptide TD-1——a magical molecule that not only enhances the permeability of the skin but also draw filamentous bacteriophages into the skin. By combining the gene fragments of antigen, immune adjuvant LTB and Luman-recruiting factor TNLFα with that of the transdermal peptide, our team got the permeable fusion  protein. In order to obtain large amount of extracelluar protein, we chose bacillus subtilis WB800N as our expression engineering bacteria. At last, the universality of our experimental method is verified by the adoption of various antigen of existing vaccine, such as HBsAg, PA and AG85B.</p>
+
<div class="atfigure" align="center" style="width:400px;font-size:14px;">Fig1. Difficult shipping in remote areas </div></div>
-
        </div>
+
<p align="justify">This year, our project focused on a revolutionary vaccine delivery. We bring a fresh Medication into the world, which contains an in situ expression system, and our product is a biological transdermal vaccine patch called T-vaccine. We chose <i>Bacillus subtilis</i> as chassis to establish the band-aid secreting fresh vaccines. The new vaccine consists of four engineering <i>B.subtilis</i>, each of which carried a gene circuit independently.</p>
-
        <div id="aim-bar" class="bassic-bar">
+
<div align="center"><img src="https://static.igem.org/mediawiki/2013/archive/e/ed/20130923171924!2013igemustc_Standardization.png" width="400" height="350" />
-
        <div class="home3col-ribbon"></div>
+
<div class="atfigure" align="center" style="width:400px;font-size:14px;">Fig2. block-based design </div></div>
-
        <div id="flag" class="title"><h1>Project Aimbitions</h1></div>
+
<p align="justify">With an excellent transdermal peptide TD1, three of the engineering <i>B.subtilis</i> could express a series of fusion proteins (the antigen and two kinds of adjuvants), which could penetrate the skin and work as traditional vaccine molecules. The fourth bacteria are our "reporter" ,which would notify users whether the band-aid works well and when the patch can be pasted . Moreover, we designed a reliable suicide system in <i>B.subtilis</i> to ensure biosafety.</p>
-
        <p>For in situ transdermal vaccine is real a giant project, we devide it into four lab parts. And we expect to achieve following goals.</p>
+
<div align="center"><img src="https://static.igem.org/mediawiki/2013/9/91/2013ustc-china_Needles.png" width="400" height="350" />
-
        <div id="aim-bar-col">
+
<div class="atfigure" align="center" style="width:400px;font-size:14px;">Fig3. No Needle </div></div>
-
        <h2>Molecule part</h2>
+
<p align="justify">T-vaccine can be stored from minus 20 to 60 Celsius, which grants its great advantage transportation and enables us to reach every remote corner of the world to help eliminate contagions from our world. It is also proved that transdermal vaccine is an effective method for a variety of pathogens such as: tuberculosis, anthrax, hepatitis B and so on. Additionally, we have created a world free from needles. Consider these advantages, T-vaccine is expected to set up a  promising vaccine research and a new development orientation.</p>
-
        <p></p></div>
+
</div>
-
        <div id="aim-bar-col">
+
-
        <h2>Protein part</h2>
+
-
        <p></p></div>
+
-
        <div id="aim-bar-col">
+
-
        <h2>Mice part</h2>
+
-
        <p></p></div>
+
-
        <div id="aim-bar-col">
+
-
        <h2>Device</h2>
+
-
        <p></p></div>
+
-
        </div>
+
</div>
</div>
<div class="rightbar">
<div class="rightbar">
-
<div class="port-sidebar-border"><h>project</h></div>
+
<div class="port-sidebar-border"><h>Project</h></div>
<div class="clear"></div>
<div class="clear"></div>
-
<div class="active" id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">overview</a></div>
+
<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
-
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">project details</a></div>
+
-
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Background">background</a></div>
+
-
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">design</a></div>
+
-
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">results</a></div>
+
-
 
+
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
 +
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
 +
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/AdvancingWork">Advancing Work</a></div>
 +
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/FutureWork">Future Work</a></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
</div></div></div>         
</div></div></div>         
          
          
</body>
</body>
</html>
</html>

Latest revision as of 12:24, 28 October 2013

Overview

In our world, billions of people are suffering from contagions while only parts of contagions can be effectively prevented by existing vaccines. The disadvantages of traditional vaccines, like being produced and purified with strict requirements on temperature, have limited their application, especially in developing countries.

Fig1. Difficult shipping in remote areas

This year, our project focused on a revolutionary vaccine delivery. We bring a fresh Medication into the world, which contains an in situ expression system, and our product is a biological transdermal vaccine patch called T-vaccine. We chose Bacillus subtilis as chassis to establish the band-aid secreting fresh vaccines. The new vaccine consists of four engineering B.subtilis, each of which carried a gene circuit independently.

Fig2. block-based design

With an excellent transdermal peptide TD1, three of the engineering B.subtilis could express a series of fusion proteins (the antigen and two kinds of adjuvants), which could penetrate the skin and work as traditional vaccine molecules. The fourth bacteria are our "reporter" ,which would notify users whether the band-aid works well and when the patch can be pasted . Moreover, we designed a reliable suicide system in B.subtilis to ensure biosafety.

Fig3. No Needle

T-vaccine can be stored from minus 20 to 60 Celsius, which grants its great advantage transportation and enables us to reach every remote corner of the world to help eliminate contagions from our world. It is also proved that transdermal vaccine is an effective method for a variety of pathogens such as: tuberculosis, anthrax, hepatitis B and so on. Additionally, we have created a world free from needles. Consider these advantages, T-vaccine is expected to set up a promising vaccine research and a new development orientation.