Team:USTC CHINA/Notebook/Protocols

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<div id="breadcrumb"><a href="">Home</a> &gt; <a href="">notebook</a> &gt; <a href="">protocols</a></div></div>
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<div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a>&gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></div></div>
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<div class="protocol-col-1">
<div class="protocol-col-1">
<h>Gene Clone</h>
<h>Gene Clone</h>
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<a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Gel Extraction"><h1>Gel Extraction</h1></a>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Gel Extraction">Gel Extraction</a></h1>
<h1>DNA digestion</h1>
<h1>DNA digestion</h1>
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<h2>Single Digestion</h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Single Digestion">Single Digestion</a></h2>
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<h2>Double Digestion</h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Double Digestion">Double Digestion</a></h2>
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<h1>Colony PCR</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Colony PCR">Colony PCR</a></h1>
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<h1>PCR</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/PCR">PCR</a></h1>
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<h1>Ligation</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Ligation">Ligation</a></h1>
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<h1>Plasmid mini-prep</h1></div>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Plasmid mini-prep">Plasmid mini-prep</a></h1></div>
<div class="protocol-col-sub">
<div class="protocol-col-sub">
<h1>Transformation</h1>
<h1>Transformation</h1>
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<h2.The transformation of E.coli</h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/The transformation of E.coli">The transformation of <i>E.coli</i></a></h2>
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<h2>The transformation of Bacillus subtilis</h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/The transformation of Bacillus subtilis">The transformation of <i>Bacillus subtilis</i></a></h2>
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<h1>Overlap PCR</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Overlap PCR">Overlap PCR</a></h1>
<h1>Extracting plasmids</h1>
<h1>Extracting plasmids</h1>
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<h2>Extracting plasmids from gram-positive bacterium</h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Extracting plasmids from gram-positive bacterium">Extracting plasmids from gram-positive bacterium</a></h2>
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<h2>Extracting plasmids from gram-negative bacterium<h2></div>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Extracting plasmids from gram-negative bacterium">Extracting plasmids from gram-negative bacterium</a><h2></div>
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<div class="protocol-col-2">
<div class="protocol-col-2">
<h>Expression of proteins</h>
<h>Expression of proteins</h>
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<h1>Expression of proteins in Bacillus subtilis</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Expression of proteins in Bacillus subtilis">Expression of proteins in <i>Bacillus subtilis</i></a></h1>
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<h1>Expression of proteins in Escherichia coli</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Expression of proteins in Escherichia coli">Expression of proteins in <i>Escherichia coli</i></a></h1>
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<h1>Concentrating proteins</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Concentrating proteins">Concentrating proteins</a></h1>
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<h>Transdermal experiments</h>
<h>Transdermal experiments</h>
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<h>Immunity&Tests</h>
<h>Immunity&Tests</h>
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<h1>ELISA</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/ELISA">ELISA</a></h1>
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<h1>Sample analysis</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Sample analysis">Sample analysis</a></h1>
</div>
</div>
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<div class="protocol-col-2">
<h>Medium</h>
<h>Medium</h>
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<h1>LB media</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/LB media">LB media</a></h1>
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<h1>10*S base</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/10*S base">10*S base</a></h1>
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<h1>HS media</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/HS media">HS media</a></h1>
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<h1>LS media</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/LS media">LS media</a></h1>
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<h1>2*YT media</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/2*YT media">2*YT media</a></h1>
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<h>Measuremen</h>
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<h1>Constitutive promoter measurements</h1>
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<h1>plac promoter response to IPTG or Glucose</h1>
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<div class="protocol-col-2">
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<h>Measurement</h>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Constitutive promoter measurements">Constitutive promoter measurements</a></h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/P-lac promoter response to IPTG or Glucose">Plac promoter response to IPTG or Glucose</a></h1>
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<div class="port-sidebar-border"><h>notebook</h></div>
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<div class="port-sidebar-border"><h>Notebook</h></div>
<div class="clear"></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">timeline</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></div>
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<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">protocols</a></div></div>
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<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></div></div>
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Latest revision as of 12:43, 28 October 2013

Gene Clone

Gel Extraction

DNA digestion

Single Digestion

Double Digestion

Colony PCR

PCR

Ligation

Plasmid mini-prep

Transformation

The transformation of E.coli

The transformation of Bacillus subtilis

Overlap PCR

Extracting plasmids

Extracting plasmids from gram-positive bacterium

Extracting plasmids from gram-negative bacterium

Expression of proteins

Expression of proteins in Bacillus subtilis

Expression of proteins in Escherichia coli

Concentrating proteins

Transdermal experiments Immunity&Tests

ELISA

Sample analysis

Medium

LB media

10*S base

HS media

LS media

2*YT media

Measurement

Constitutive promoter measurements

Plac promoter response to IPTG or Glucose

Notebook

Retrieved from "http://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols"