Team:USTC CHINA/Notebook/Protocols/Colony PCR
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*Volumes of 25 mM MgCl2, required for specific final MgCl2 concentration:</br> | *Volumes of 25 mM MgCl2, required for specific final MgCl2 concentration:</br> | ||
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3. Gently vortex the samples and spin down.</br> | 3. Gently vortex the samples and spin down.</br> | ||
4. Perform PCR using recommended thermal cycling conditions:</br> | 4. Perform PCR using recommended thermal cycling conditions:</br> | ||
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Latest revision as of 08:56, 27 September 2013