"
Team:USTC CHINA/Notebook/Protocols/Gel Extraction
From 2013.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 8: | Line 8: | ||
<div class="container" align="left"> | <div class="container" align="left"> | ||
<div id="igemlogo"><a href="https://2013.igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2013/2/26/2013ustcigem_IGEM_basic_Logo.png" alt="igem home page" width="50" height="40" /></a></div> | <div id="igemlogo"><a href="https://2013.igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2013/2/26/2013ustcigem_IGEM_basic_Logo.png" alt="igem home page" width="50" height="40" /></a></div> | ||
| - | + | <ul id="nav"> | |
| - | + | ||
<li><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a></li> | <li><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a></li> | ||
<li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a> | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a> | ||
| Line 16: | Line 15: | ||
<li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></li> | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></li> | ||
<li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li> | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li> | ||
| - | <li><a href="https://2013.igem.org/Team:USTC_CHINA | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| Line 27: | Line 26: | ||
<li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a> | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a> | ||
<ul class="subs"> | <ul class="subs"> | ||
| - | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></li> |
<li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></li> | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></li> | ||
| - | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs of Immune Experiments</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| Line 38: | Line 37: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| - | <li><a href="https://2013.igem.org/Team:USTC_CHINA/ | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Team</a> |
<ul class="subs"> | <ul class="subs"> | ||
| - | <li><a href="https://2013.igem.org/Team:USTC_CHINA/ | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Members</a></li> |
<li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></li> | <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></li> | ||
| - | <li><a href="https://2013.igem.org/Team:USTC_CHINA/ | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></li> |
| - | <li><a href="https:// | + | <li><a href="https://igem.org/2013_Judging_Form?id=1074#iGEM_Medals">Achievements</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| Line 60: | Line 59: | ||
<h1>Gel Extraction</h1> | <h1>Gel Extraction</h1> | ||
<p>Performed with AxyPrep 96-well DNA Gel Extraction Kit (type: AP-96-GX) | <p>Performed with AxyPrep 96-well DNA Gel Extraction Kit (type: AP-96-GX) | ||
| - | Protocol | + | Protocol<br> |
1. Excise gel slice containing DNA fragment of interest.</br> | 1. Excise gel slice containing DNA fragment of interest.</br> | ||
| Line 88: | Line 87: | ||
</div> | </div> | ||
</div> | </div> | ||
| + | <br><br> | ||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 08:51, 27 September 2013
Gel Extraction
Performed with AxyPrep 96-well DNA Gel Extraction Kit (type: AP-96-GX)
Protocol
1. Excise gel slice containing DNA fragment of interest.
2. Add 3×sample volume of Buffer DE-A.
Incubate at 75° C for 15-20 min or until gel melts completely.
Add 0.5 × Buffer DE-A volume of Buffer DE-B.
3. Binding sample DNA(Centrifuge at 5,000 - 6,000×g for 5 min).
4. Add 800 μl of Buffer W2 (5,000 - 6,000×g for 1 min).
Repeat wash with Buffer W2.
Centrifuge empty plate for 5 min at 5,000 - 6,000×g to remove residue W2.
5. Elute with 50 μl of Eluent or Deionized Water.(5,000 - 6,000×g, 5min).
