Team:SCAU-China

From 2013.igem.org

(Difference between revisions)

Latest revision as of 22:21, 27 September 2013

Overview

Organophosphorus (OP) compounds, which are highly toxic contaminants in agro-environment and food security, have been widely applied to pesticides. Parathion is a typical representative of organophosphorus pesticides. Residues of OP pesticides in food are also often reported.So, this year, our goal is to construct a system that can sense and degrade OP pesticides.

View Our Work →

Our Project

OP Degradation

The bacterial organophosphorus hydrolase enzyme hydrolyzes and detoxifies a broad range of toxic organophosphate pesticides. In the first part, we need to use these enzymes to degrade parathion into PNP.
PNP Sensor

The second part is a biosensor of the hydrolysis product P-Nitrophenol (PNP). There is no operon that can react to parathion. We foucus on constructing a PNP sensor, which can reflect the existence of parathion.
PNP Degradation

We also design a PNP degradation system. We use enzymes NphA1 and NphA2 to degrade PNP, but fail. We find another pathway to degrade PNP in 5 steps with 4 enzymes in Rhodococcus sp. Strain PN1.
Suicide System

With the public increasingly concerned about biosafety, we conceived a suicide gene circuit to ensure these recombinanational bacteria can only survive in laboratories and not cause horizontal gene transfer.

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+    <!-- #logo -->
+    <div id="logo">
+      <a> <img src="https://static.igem.org/mediawiki/2013/6/65/SCAU-china_logo_.png"> </a>
+    </div>
+    <div id="logo">
+      <a> <img src="https://static.igem.org/mediawiki/2013/7/74/SCAU-China_igemlogo.png"> </a>
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+    <nav id="primary-nav" role="navigation" class="clearfix">
+      <ul id="menu-primary-nav" class="nav sf-menu clearfix">
+        <li class="current-menu-item"><a href="https://2013.igem.org/Team:SCAU-China" >Home</a></li>
+        <li><a href="#">Team</a>
+          <ul class="sub-menu">
+            <li><a href="https://2013.igem.org/Team:SCAU-China/Team" >Members</a></li>
+            <li><a href="https://2013.igem.org/Team:SCAU-China/Attributions" >Attributions</a></li>
+          </ul>
+        </li>
+        <li><a href="#">Project</a>
+          <ul class="sub-menu">
+            <li><a href="https://2013.igem.org/Team:SCAU-China/Project" >Overview</a></li>
+            <li><a href="https://2013.igem.org/Team:SCAU-China/Project/OPdegradation" >OP Degradation</a></li>
+            <li><a href="https://2013.igem.org/Team:SCAU-China/Project/PNPsensor" >PNP Sensor</a></li>
+            <li><a href="https://2013.igem.org/Team:SCAU-China/Project/PNPdegradation" >PNP Degradation</a></li>
+            <li><a href="https://2013.igem.org/Team:SCAU-China/Project/SuicideSystem" >Suicide System</a></li>
+            <li><a href="https://2013.igem.org/Team:SCAU-China/Notebook">Notes</a></li>
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+            <li><a href="https://2013.igem.org/Team:SCAU-China/Parts" >Biobricks</a></li>
+            <li><a href="https://2013.igem.org/Team:SCAU-China/Achievements" >Achievements</a></li>
+            <li><a href="https://2013.igem.org/Team:SCAU-China/Safety" >Safety</a></li>
+          </ul>
+        </li>
+        <li><a href="#">Human Practice</a>
+          <ul class="sub-menu">
+            <li><a href="https://2013.igem.org/Team:SCAU-China/HP/Survey">Survey</a></li>
+            <li><a href="https://2013.igem.org/Team:SCAU-China/HP/Communication">Communication</a></li>
+            <li><a href="https://2013.igem.org/Team:SCAU-China/HP/Game">card Game</a></li>
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+    <!-- #content -->
+    <section id="content1" role="main" class="fullwidth">
+      <div id="feature" class="clearfix">
+        <div id="callout">
+          <h2>Overview</h2>
+          <p>&nbsp;&nbsp;&nbsp;&nbsp;Organophosphorus (OP) compounds, which are highly toxic contaminants in agro-environment and food security, have been widely applied to pesticides. Parathion is a typical representative of organophosphorus pesticides. Residues of OP pesticides in food are also often reported.So, this year, our goal is to construct a system that can sense and degrade OP pesticides.</p>
+          <p><a href="https://2013.igem.org/Team:SCAU-China/Project">View Our Work &rarr;</a></p>
+        </div>
+        <div id="hpslider" class="flexslider">
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+          <ul class="slides">
+            <li><img src="https://static.igem.org/mediawiki/2013/1/12/SCAU-China_slide1.jpg" alt="" /> </li>
+            <li><img src="https://static.igem.org/mediawiki/2013/2/26/SCAU-China_slide2.jpg" alt="" /> </li>
+            <li><img src="https://static.igem.org/mediawiki/2013/0/0d/SCAU-China_slide3.jpg" alt="" /> </li>
+          </ul>
+        </div>
+      </div>
+      <div id="home-blocks" class="clearfix">
+        <h3 class="deco"><span class="outer"><span class="inner">Our Project</span></span></h3>
+        <ul class="clearfix">
+          <li>
+            <h3><a href="https://2013.igem.org/Team:SCAU-China/Project/OPdegradation">OP Degradation</a></h3>
+            <p>&nbsp;&nbsp;&nbsp;&nbsp;The bacterial organophosphorus hydrolase enzyme hydrolyzes and detoxifies a broad range of toxic organophosphate pesticides. In the first part, we need to use these enzymes to degrade parathion into PNP.</p>
+          </li>
+          <li>
+            <h3><a href="https://2013.igem.org/Team:SCAU-China/Project/PNPsensor" >PNP Sensor</a></h3>
+            <p>&nbsp;&nbsp;&nbsp;&nbsp;The second part is a biosensor of the hydrolysis product P-Nitrophenol (PNP). There is no operon that can react to parathion. We foucus on constructing a PNP sensor, which can reflect the existence of parathion.</p>
+          </li>
+          <li>
+            <h3><a href="https://2013.igem.org/Team:SCAU-China/Project/PNPdegradation" >PNP Degradation</a></h3>
+            <p>&nbsp;&nbsp;&nbsp;&nbsp;We also design a PNP degradation system. We use enzymes NphA1 and NphA2 to degrade PNP, but fail. We find another pathway to degrade PNP in 5 steps with 4 enzymes in Rhodococcus sp. Strain PN1.            </p>
+          </li>
+          <li>
+            <h3><a href="https://2013.igem.org/Team:SCAU-China/Project/SuicideSystem" >Suicide System</a></h3>
+            <p>&nbsp;&nbsp;&nbsp;&nbsp;With the public increasingly concerned about biosafety, we conceived a suicide gene circuit to ensure these recombinanational bacteria can only survive in laboratories and not cause horizontal gene transfer.</p>
+          </li>
+        </ul>
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