Team:TU-Eindhoven/Attributions

Attributions

The work for this project was fully performed by the iGEM TU-Eindhoven 2013 team members, the lab work started from June 2013 onwards.

The idea of the CEST protein producing bacteria was developed by the team way before starting the actual lab work, it was the result of thorough literature research and discussions. All the experiments, the modeling and the device design were planned and executed by the team. Our advisers gave us guidance when we required it, but their function was merely as counselors. We receive lab guidance from Stijn Aper, who is a PhD student at TU Eindhoven. He supervised our lab work, especially in the beginning of the project, and gave us valuable advices whenever we encountered an issue with the outcome of the experiments.

The human practices project was completely developed by the team with the collaboration of 6 iGEM teams from across the world. The teams were asked to revise and classify a common statement about Synthetic Biology as true, false, or inconclusive. These teams were also asked to write a brief discussion piece about the statement and the arguments behind the final classification given to the statement.

The team managed all the organizational matters, including sponsoring. We made our own logos, designs and drawings, except for those found on the safety section of our wiki which were made by the industrial designer Mariana Félix. The wiki, presentation and poster style were also designed and developed by the team, but Mariana Félix helped us with the aesthetic details of the poster and presentation.

The Escherichia coli K12 strain, MG1655 which we required for the chassis of our device was provided by Groningen University.

The following table presents an overview of what each team member has been responsible for:

Ardjan Lab Captain		Management Management of the whole lab team Lab Journal Author of Lab Journal Parts Documentation Responsible for all BioBrick documentation, both characterization and safety Safety Responsible for the safety forms belonging to each of the BioBricks
Pascal Modeling Captain		Management Management of the whole modeling team Wiki Responsible for the design and functionality of the wiki Human Practice Website setup and design and technical implementation. Wet Lab Construct design, IT support for construct design and general lab work preparation. Dry Lab Contrast agent selection by database probing and Molecular Dynamics simulations. Wet Lab MRI data processing and analysis. Dry Lab Implementation of the stochastic and ODE models of the Decoy Sites and FNR. Wet Lab Getting the BioReactor back to work and carrying out the anaerobic experiments and general help with the lab work.
Zandra Modeling		Wiki Responsible for the content the project, safety, society, and notebook sections of the wiki. Safety Responsible for the research of safety locks implementation on our device. Human Practice Responsible for determining and categorizing the initial facts for the fact checker project.
Nick Lab		Human Practice Management of the Human Practice project External contacts Responsible for the communications between other teams and instances Outreach Responsible for the publicity for our project Lab Responsible for the creation of the Biobricks
Yicong Modeling		Modeling Responsible for creating and updating the models. Wiki Responsible for the content of modeling.
Jacques Lab		Human Practice Helped with the Human Practice Finance Sponsoring Lab Responsible for the creation of the Biobricks
Sander Lab		Lab Assistance with aerobic protein expression Finance Treasury, Sponsoring & Logistics

Acknowledgements

We want to thank everybody who helped us during the project, especially:

Our supervisors and advisers Maarten Merkx, Tom de Greef, and Natal van Riel for their advice on the lab work and modeling throughout the project, and Stijn Aper for his guidance in the lab.

Groningen University for providing us with the bacterial strain we required for our device.

The industrial designer Mariana Felix for help with the aesthetics of our poster and presentation.

The TU/e Biomedical NMR group, in particular ir. Stephanie Hectors and Prof.dr. Klaas Nicolaij for their help with the MRI experiments and data analysis.

and finally

The Institute for Complex Molecular Systems for their support throughout the project. Especially Sagitta Peters MSc., for her help with the finances and facilities, dr. ir. Koen Pieterse and researchers from the Institute for giving us a fresh perspective on different aspects of our project, including the presentation.