"
Team:BYU Provo/Notebook/Cholera - Enzymes/May-June
From 2013.igem.org
(Difference between revisions)
Sabinater09 (Talk | contribs) |
Mschellhous (Talk | contribs) |
||
| (13 intermediate revisions not shown) | |||
| Line 18: | Line 18: | ||
<font size = "4"> | <font size = "4"> | ||
| - | : <u> '''Cholera | + | : <u> '''Cholera Enzyme''' </u> </font> |
: [[Team:BYU Provo/Notebook/Cholera - Enzymes/March-April|March-April]] | : [[Team:BYU Provo/Notebook/Cholera - Enzymes/March-April|March-April]] | ||
| Line 26: | Line 26: | ||
: [[Team:BYU Provo/Notebook/Cholera - Enzymes/July-August|July-August]] | : [[Team:BYU Provo/Notebook/Cholera - Enzymes/July-August|July-August]] | ||
| - | : [[Team:BYU Provo/Notebook/Cholera - Enzymes/September-October|September]] | + | : [[Team:BYU Provo/Notebook/Cholera - Enzymes/September-October|September-October]] |
: [[Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols|Protocols]] | : [[Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols|Protocols]] | ||
| Line 38: | Line 38: | ||
<br> | <br> | ||
| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> We started contacting teams that have worked with Savinase and DspB. We started the initial work of purifying AmyA which was available at BYU. As well we started outlining the biofilm degradation assay using positive and negative controls. </font> |
<br> | <br> | ||
| Line 50: | Line 50: | ||
<br> | <br> | ||
| - | | style="width: 20%; background-color: transparent;"| [[File: | + | | style="width: 20%; background-color: transparent;"| [[File:BYUCEFall1.JPG|250px|center]] |
|- | |- | ||
| Line 60: | Line 60: | ||
<br> | <br> | ||
| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> Continued our work of cloning AmyA and Savinase into E. coli. Ran initial biofilm degradation assays using urea as a positive control. </font> |
<br> | <br> | ||
| Line 71: | Line 71: | ||
| - | | style="width: 20%; background-color: transparent;"| [[File: | + | | style="width: 20%; background-color: transparent;"| [[File:BiohazardBYU2013.jpg|250px|center]] |
|- | |- | ||
| Line 81: | Line 81: | ||
<br> | <br> | ||
| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> We are still having issues with AmyA and Savinase. We are starting to fine tune our biofilm degradation assay. </font> |
<br> | <br> | ||
| Line 92: | Line 92: | ||
| - | | style="width: 20%; background-color: transparent;"| [[File: | + | | style="width: 20%; background-color: transparent;"| [[File:Michael_pipetteFinal.jpg|250px|center]] |
|- | |- | ||
| Line 102: | Line 102: | ||
<br> | <br> | ||
| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> After a few more failed attempts with Savinase we are restarting the isolation from ''B. subtilis.'' AmyA is almost finished and ready for purification. The biofilm degradation assay is still being fine tuned. </font> |
<br> | <br> | ||
| Line 113: | Line 113: | ||
| - | | style="width: 20%; background-color: transparent;"| [[File: | + | | style="width: 20%; background-color: transparent;"| [[File:BYUCEWinter5.JPG|250px|center]] |
Latest revision as of 00:08, 28 September 2013
| ||
|
|
May 1 - May 14
We started contacting teams that have worked with Savinase and DspB. We started the initial work of purifying AmyA which was available at BYU. As well we started outlining the biofilm degradation assay using positive and negative controls.
|  |
| May 15 - May 31
Continued our work of cloning AmyA and Savinase into E. coli. Ran initial biofilm degradation assays using urea as a positive control.
|  | |
| June 1 - June 14
We are still having issues with AmyA and Savinase. We are starting to fine tune our biofilm degradation assay.
|  | |
| June 15 - June 30
After a few more failed attempts with Savinase we are restarting the isolation from B. subtilis. AmyA is almost finished and ready for purification. The biofilm degradation assay is still being fine tuned.
| 
| |
