Team:USTC CHINA/Attributions

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                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
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                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></li>
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                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
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<div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div></div>
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<div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Team">Team</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></div></div>
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         <h1>Overview</h1>
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         <h1>Attributions</h1>
          
          
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In the last two months,thanks to our devotion and the support of various professors and scientific institutions ,our team achieved proud results.<br>
In the last two months,thanks to our devotion and the support of various professors and scientific institutions ,our team achieved proud results.<br>
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In the summer vacation,we  use the gene material donated by scientific institution and individuals to  standardize and splice.we standardize and splice transdermal peptide  and gene circuits consists of various fusion protein of antigen.Meanwhile,we expressed TD1-GFP、TD1-HBsAg、TD1-LTB and all of expression of them was verified by in the E.coli. TD1-PA、TD1-HBsAg、TD1-Ag85b and TD1-LTB constructed circuits of the secretion vector,WB800N.Firstly,the transdermal experiment outside human body succeeded.Secondly,we also try to do experiment in living mice.Generally,we construct 16 new parts,we set a foundation for toolkit.Also our standardized BBa_K1074006 made a frame for other secretion expression researchers,theoretically users can use TD1 to merge any protein,to made the target protein have the function of secretion and transdermal permeation.<br>
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In the summer vacation,we  used the gene material donated by scientific institutions and individuals to  standardize and splice.we standardized and spliced transdermal peptide  and gene circuits consists of various fusion protein of antigen.Meanwhile,we expressed TD1-GFP、TD1-HBsAg、TD1-LTB and all of expression of them was verified by in the <i>E.coli</i>. TD1-PA、TD1-HBsAg、TD1-Ag85b and TD1-LTB constructed circuits of the secretion vector,WB800N.Firstly,the transdermal experiment outside human body succeeded.Secondly,we also try to do experiment in living mice.Generally,we construct 16 new parts,we set a foundation for toolkit.Also our standardized BBa_K1074006 set a frame for other secretion expression researchers,theoretically users can use TD1 to merge any protein,to made the target protein have the function of secretion and transdermal permeation.<br>
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Thanks to the following individuals and institutions:
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Thanks to the following individuals and institutions:<br>
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1.gene donation
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1.Gene donation<br>
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Standardized antigen:Chinese Centre for Disease
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Standardized antigen:Chinese Centre for Disease<br>
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TD-1 short chain poly peptide:Professor Longping Wen
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TD-1 short chain poly peptide:Professor Longping Wen<br>
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2.theoretically support:
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2.Theoretically support:<br>
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Consultation of Bacillus subtilis:Professor Xin Run,Nanjing Agriculture University
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Consultation of <i>Bacillus subtilis</i>:Professor Xin Run,Nanjing Agriculture University<br>
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Consultation of transdermal  experiment:Professor Longping Wen
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Consultation of transdermal  experiment:Professor Longping Wen<br>
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Training of mice experiment:Professor Ting Yue
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Training of mice experiment:Professor Ting Yue<br>
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3.Preliminary Partner:Hualan Biology Co.,ltd.
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3.Preliminary Partner:Hualan Biology Co.,ltd.<br>
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<div class="port-sidebar-border"><h>Team</h></div>
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<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Members</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></div>
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<div id="t1"><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Background">Background</a></div>
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<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">Design</a></div>
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<div id="t1"><a href="https://igem.org/2013_Judging_Form?id=1074#iGEM_Medals">Achievements</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results/FurthurWork">Furthur Work</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
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Latest revision as of 12:44, 28 October 2013

Attributions

In the last two months,thanks to our devotion and the support of various professors and scientific institutions ,our team achieved proud results.
In the summer vacation,we used the gene material donated by scientific institutions and individuals to standardize and splice.we standardized and spliced transdermal peptide and gene circuits consists of various fusion protein of antigen.Meanwhile,we expressed TD1-GFP、TD1-HBsAg、TD1-LTB and all of expression of them was verified by in the E.coli. TD1-PA、TD1-HBsAg、TD1-Ag85b and TD1-LTB constructed circuits of the secretion vector,WB800N.Firstly,the transdermal experiment outside human body succeeded.Secondly,we also try to do experiment in living mice.Generally,we construct 16 new parts,we set a foundation for toolkit.Also our standardized BBa_K1074006 set a frame for other secretion expression researchers,theoretically users can use TD1 to merge any protein,to made the target protein have the function of secretion and transdermal permeation.

Thanks to the following individuals and institutions:
1.Gene donation
Standardized antigen:Chinese Centre for Disease
TD-1 short chain poly peptide:Professor Longping Wen
2.Theoretically support:
Consultation of Bacillus subtilis:Professor Xin Run,Nanjing Agriculture University
Consultation of transdermal experiment:Professor Longping Wen
Training of mice experiment:Professor Ting Yue
3.Preliminary Partner:Hualan Biology Co.,ltd.