Team:USTC CHINA/Parts

From 2013.igem.org

(Difference between revisions)
 
(17 intermediate revisions not shown)
Line 5: Line 5:
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:USTC_CHINA/pro.css?action=raw&ctype=text/css" />
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:USTC_CHINA/pro.css?action=raw&ctype=text/css" />
<style type="text/css">
<style type="text/css">
-
.part-col-1{float:left;width:290px;padding:10px;}
+
.part-col-1{float:left;width:290px;padding:10px;height:200px;}
-
.part-col-1 h1{line-height:24px;}
+
.part-col-1 h1{line-height:24px;height:40px;}
-
.part-col-2{float:left;width:145px;padding:5px;}
+
.part-col-2{float:left;width:145px;padding:5px;height:280px;}
 +
.part-col-2 h1{height:40px;line-height:20px;}
 +
 
</style>
</style>
</head>
</head>
Line 20: Line 22:
             <ul class="subs">
             <ul class="subs">
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
-
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></li>
+
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
Line 64: Line 67:
<div class="conbar2">
<div class="conbar2">
<div class="leftbar" align="left" style="margin-bottom:30px;">
<div class="leftbar" align="left" style="margin-bottom:30px;">
-
        <div class="part-col-1">
+
 
 +
 
 +
<div class="part-col-1">
         <h1>TD1, Transdermal peptide</h1>
         <h1>TD1, Transdermal peptide</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074000">BBa_K1074000</a></h2>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074000">BBa_K1074000</a></h2>
-
         <p>TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin. Studies suggested that the peptide creates a transient opening in the skin barrier to enable macromolecular material to reach systemic circulation.</p>
+
         <p align="center">TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin...</p>
</div>
</div>
Line 73: Line 78:
         <h1>Pgrac+RBS+SamyQ+TD1<br>+GFP</h1>
         <h1>Pgrac+RBS+SamyQ+TD1<br>+GFP</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074006">BBa_K1074006</a></h2>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074006">BBa_K1074006</a></h2>
-
         <p>This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins via a modular PCR or standard cut/ligation method.</p>
+
         <p align="center">This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins...</p>
</div>
</div>
-
<div class="clear"></div>
+
 
-
  <div class="part-col-1">
+
  <div class="part-col-2">
         <h1>PHT43</h1>
         <h1>PHT43</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2>
-
         <p>PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis. </p>
+
         <p align="center">PHT43 is a <i>E.coli-B.subtilis</i> shuttle vector allowing high-level expression of secreted proteins in <i>B.subtilis</i>... </p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>HBsAg</h1>
         <h1>HBsAg</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074002">BBa_K1074002</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074002">BBa_K1074002</a></h2>  
-
         <p>HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection.</p>
+
         <p align="center">HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection...</p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>Ag85b</h1>
         <h1>Ag85b</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074003">BBa_K1074003</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074003">BBa_K1074003</a></h2>  
-
         <p>The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). </p>
+
         <p align="center">The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin... </p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>Protective Antigen Domain 4</h1>
         <h1>Protective Antigen Domain 4</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074004">BBa_K1074004</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074004">BBa_K1074004</a></h2>  
-
         <p>Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax. </p>
+
         <p align="center">Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax... </p>
</div>
</div>
-
<div class="clear"></div>
+
 
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>LTB</h1>
         <h1>LTB</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074005">BBa_K1074005</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074005">BBa_K1074005</a></h2>  
-
         <p>This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli.</p>
+
         <p align="center">This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of <i>E.coli</i>...</p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
-
         <h1>Pctc+RBS+amilCP</h1>
+
         <h1>Pctc+RBS+<br>amilCP</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2>  
-
         <p>Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response). </p>
+
         <p align="center">Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in <i>B.subtilis</i> activated by endogenous sigma factor B ...</p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
-
         <h1>SdpA,SdpB,SpbC</h1>
+
         <h1>SdpA,SdpB,<br>SpbC</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2>  
-
         <p>Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation.</p>
+
         <p align="center">Killing factor of <i>B.subtilis</i> ,SpbC Induces the lysis of other <i>B.subtilis</i> cells that have not entered the sporulation pathway...</p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>Pgrac+RBS+SDP</h1>
         <h1>Pgrac+RBS+SDP</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2>  
-
         <p>Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of Bacillus subtilis.</p>
+
         <p align="center">Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of <i>B.subtilis</i>...</p>
</div>
</div>
<div class="clear"></div>
<div class="clear"></div>
-
<div class="part-col-1">
+
<div class="part-col-2">
-
         <h1>PsdpRI+RBS+SDP</h1>
+
         <h1>PsdpRI+RBS<br>+SDP</h1>
       <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2>  
       <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2>  
-
         <p>We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose.Killing is mediated by the exported toxic protein SpbC. </p>
+
         <p align="center">We construct this gene circuit as our kill switch to kill the engineered <i>B.subtilis</i> for the safety purpose...</p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
          
          
Line 132: Line 137:
         <h1>Pgrac promoter</h1>
         <h1>Pgrac promoter</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074012">BBa_K1074012</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074012">BBa_K1074012</a></h2>  
-
         <p>Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG. </p>
+
         <p align="center">Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG... </p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>promoter sdpR/I</h1>
         <h1>promoter sdpR/I</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074013">BBa_K1074013</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074013">BBa_K1074013</a></h2>  
-
         <p>This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis ,and it contains an operon for the signaling pathway of sdpc,a bacterial toxin of B.subtilis. </p>
+
         <p align="center">This part is a sigma factor A-dependent promotor of the gene sdpR derived from the <i>B.subtilis</i>...</p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>SamyQ</h1>
         <h1>SamyQ</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074014">BBa_K1074014</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074014">BBa_K1074014</a></h2>  
-
         <p>SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in Bacillus subtilis WB800N. Fuse it with the recombinant proteins to obtain the secretion of recombinant proteins in Bacillus subtilis.</p>
+
         <p align="center">SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in <i>B.subtilis</i> WB800N...</p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>RBS for Pgrac</h1>
         <h1>RBS for Pgrac</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074015">BBa_K1074015</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074015">BBa_K1074015</a></h2>  
-
         <p>An efficient RBS for promoter Pgrac(BBa_K1074012) in Bacillus subtilis. We used it in all of our gene circuits containing promoter Pgrac. </p>
+
         <p align="center">An efficient RBS for promoter Pgrac(BBa_K1074012) in <i>B.subtilis</i>.../p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>RBS for Pctc</h1>
         <h1>RBS for Pctc</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074016">BBa_K1074016</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074016">BBa_K1074016</a></h2>  
-
         <p>This is an efficient RBS for promoter Pctc(BBa_K143010) in Bacillus subtilis. It's also an universal RBS for promoters in Bacillus subtilis. We also used it with the promoter PsdpRI(BBa_K1074013) in our project. </p>
+
         <p align="center">This is an efficient RBS for promoter Pctc(BBa_K143010) in <i>B.subtilis</i>...</p>
</div>
</div>
Line 170: Line 175:
<div class="clear"></div>
<div class="clear"></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
-
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></div>
+
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></div>
-
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Background">Background</a></div>
+
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></div>
-
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">Design</a></div>
+
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
-
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results/FurthurWork">Furthur Work</a></div>
+
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/AdvancingWork">Advancing Work</a></div>
 +
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/FutureWork">Future Work</a></div>
<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
</div></div></div>         
</div></div></div>         

Latest revision as of 12:33, 28 October 2013

TD1, Transdermal peptide

BBa_K1074000

TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin...

Pgrac+RBS+SamyQ+TD1
+GFP

BBa_K1074006

This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins...

PHT43

BBa_K1074001

PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis...

HBsAg

BBa_K1074002

HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection...

Ag85b

BBa_K1074003

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin...

Protective Antigen Domain 4

BBa_K1074004

Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax...

LTB

BBa_K1074005

This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli...

Pctc+RBS+
amilCP

BBa_K1074007

Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B.subtilis activated by endogenous sigma factor B ...

SdpA,SdpB,
SpbC

BBa_K1074009

Killing factor of B.subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway...

Pgrac+RBS+SDP

BBa_K1074010

Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of B.subtilis...

PsdpRI+RBS
+SDP

BBa_K1074011

We construct this gene circuit as our kill switch to kill the engineered B.subtilis for the safety purpose...

Pgrac promoter

BBa_K1074012

Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG...

promoter sdpR/I

BBa_K1074013

This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis...

SamyQ

BBa_K1074014

SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in B.subtilis WB800N...

RBS for Pgrac

BBa_K1074015

An efficient RBS for promoter Pgrac(BBa_K1074012) in B.subtilis.../p>

RBS for Pctc

BBa_K1074016

This is an efficient RBS for promoter Pctc(BBa_K143010) in B.subtilis...

Project

Retrieved from "http://2013.igem.org/Team:USTC_CHINA/Parts"