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Experiment 4: Glyoxylate Shunt
Introduce glyoxylate enzymes responsible for the shunt
Team:Hong Kong HKUST/experiment
From 2013.igem.org
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<br><br><br><div id="slide"><h1>Experiments</h1><p id="yo"><i><b>Summary of experiments done this summer</b></i> - The HKUST iGEM 2013 carried out a lot of experiments this summer, including regular plasmid construction, PCR, and mutagenesis. We listed the main experiments done under each module in a clear, forward way. </p><br></div> | <br><br><br><div id="slide"><h1>Experiments</h1><p id="yo"><i><b>Summary of experiments done this summer</b></i> - The HKUST iGEM 2013 carried out a lot of experiments this summer, including regular plasmid construction, PCR, and mutagenesis. We listed the main experiments done under each module in a clear, forward way. </p><br></div> | ||
| - | <div id="interview1"><h3>Experiment 1: Fatty Acid | + | <div id="interview1"><h3>Experiment 1: Cell Viability & Fatty Acid Quantification</h3><div id="pre-img"><img src="https://static.igem.org/mediawiki/2013/f/fa/Expt0.png" style="padding-left:2px;padding-top:5px;width:90%;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp1"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="pre-desc"><p id="pre-desc-text">Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate</p></div></div> |
| - | <div id="interview"><h3>Experiment 2: Fatty Acid Sensing Mechanism</h3><div id="pre-img"><img src="https://static.igem.org/mediawiki/2013/4/4f/Expt1.png" style="padding-left:5px;padding-top:5px;width:90%;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/ | + | <div id="interview"><h3>Experiment 2: Fatty Acid Sensing Mechanism</h3><div id="pre-img"><img src="https://static.igem.org/mediawiki/2013/4/4f/Expt1.png" style="padding-left:5px;padding-top:5px;width:90%;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp2"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="pre-desc"><p id="pre-desc-text">Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration</p></div></div> |
| - | <div id="presentation"><h3>Experiment 3: Protein Trafficking</h3><div id="pre-img"><img src="https://static.igem.org/mediawiki/2013/4/45/Expt2.png" style="padding-left:5px;width:105%;padding-top:8px;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/ | + | <div id="presentation"><h3>Experiment 3: Protein Trafficking</h3><div id="pre-img"><img src="https://static.igem.org/mediawiki/2013/4/45/Expt2.png" style="padding-left:5px;width:105%;padding-top:8px;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp3"><br><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:75%;height:40px;padding-top:2px;"></a></center></div><div id="pre-desc"><p id="pre-desc-text">Target ACE proteins into mitochondria</p></div></div> |
| - | <div id="article"><h3>Experiment 4: Glyoxylate Shunt</h3><div id="art-img"><img src="https://static.igem.org/mediawiki/2013/c/c0/Expt3.png" style="padding-left:5px;width:90%;padding-top:5px;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/ | + | <div id="article"><h3>Experiment 4: Glyoxylate Shunt</h3><div id="art-img"><img src="https://static.igem.org/mediawiki/2013/c/c0/Expt3.png" style="padding-left:5px;width:90%;padding-top:5px;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp4"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="art-desc"><p id="art-desc-text">Introduce glyoxylate enzymes responsible for the shunt</p></div></div> |
Latest revision as of 18:57, 27 September 2013
Experiments
Summary of experiments done this summer - The HKUST iGEM 2013 carried out a lot of experiments this summer, including regular plasmid construction, PCR, and mutagenesis. We listed the main experiments done under each module in a clear, forward way.
Experiment 1: Cell Viability & Fatty Acid Quantification
Measuring cell viability at different fatty acid concentration & measure fatty acid uptake rate
Experiment 2: Fatty Acid Sensing Mechanism
Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration
Experiment 3: Protein Trafficking
Target ACE proteins into mitochondria
