Team:TU-Delft

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<h2>Summary</h2>
 
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<p align="justify">Methicillin-Resistant Staphylococcus Aureus is a bacterium responsible for several difficult-to-treat infections in humans. MRSA can be any strain of Staphylococcus aureus that has developed, through the process of natural selection, resistance to beta-lactam antibiotics. MRSA is especially troublesome in hospitals, prisons and nursing homes, where patients with open wounds, invasive devices, and weakened immune systems are at greater risk of infection than the general public. The Centers for Disease Control and Prevention estimated that about 1.7 million infections occurred in the United States in 2002. More than 30% MRSA cases of total number of Staphyllococcus infections are in Europe. </p>
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<p align="justify" margin: 20px >Several newly discovered strains of MRSA show antibiotic resistance even to vancomycin and teicoplanin. These new evolutions of the MRSA bacterium are called Vancomycin intermediate-resistant Staphylococcus aureus (VISA). There have been claims that bacteriophage can be used to cure MRSA.  
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<p align="justify">For that reason we decided to use a different approach to deal with this MRSA problem.More specifically, the project would focus on killing the Staphylococcus species by antimicrobial peptides synthesized in an E.coli model organism.  </p>
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<p align="justify">However, we will not only focus on a new type of antibiotic molecule (the peptides) but also on a smart way to deliver them. In particular, our project consists of three main phases:</p>
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<p align="center"><b>Sense - Produce - Kill</b></br>
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<p align="justify">At the beginning, E.Coli detects S. Aureus through a quorum sensing mechanism. Afterwards a timer is activated and the peptide is produced . The existence of the timer is related to efficient delivery of high peptide concentration. By the end of the production, the produced peptide kills S.Aureus. The final step is the activation of a kill switch in order E.Coli to be killed. In that way, we  ensure the safety of our system.
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    <h2>Who are we?</h2><div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">We are the iGEM 2013 team of Delft University of Technology, Netherlands. We are working on the production of antimicrobial peptides in order to kill methicillin resistant <i>Staphylococcus aureus, MRSA</i>.</p>
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<li> <h2>Aim</h2><div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> To engineer <i>Escherichia coli</i> that can detect MRSA in order to locally produce and deliver antimicrobial peptides. The project is divided into several essential modules.
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    <h2>Sensing autoinducing peptides</h2> 
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    <div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">The sensing part of the of <i>Staphylococcus aureus</i> Agr system is engineered in <i>E. coli</i>. In that way <i>E. coli</i> detects the AIPs that are produced by <i>Staphylococcus aureus</i> which  induces the peptide production. </p>
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    <h2>Peptide Production</h2>
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<div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> <i>E. coli</i> detects <i>Staphylococcus aureus</i> by its own quorum sensing system, and at that moment inactivated antimicrobial peptides are  being produced </p>
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    <h2>Timer</h2>
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With the use of a timer the peptide will be activated by cleavage of an inactivating tag. With the use of a timer, high concentrations of peptide can be delivered very locally in order to efficiently kill MRSA.</p>
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    <h2>Kill Switch</h2>
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In order to ensure the safety of the system, the peptide activating protease is combined with a kill switch that will kill our E. coli.</p>
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    <h2>Zephyr</h2>
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Zephyr is a low-cost Do It Yourself (DIY) machine which can scan petridishes and 96 well plates for expression of fluorescence at micrometer scale.
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<a href="https://2013.igem.org/Team:TU-Delft/Team">1</a>
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<p> <h3> Check out what news in Netherlands has to say about the achievements of our Team </p><a href="https://2013.igem.org/Team:TU-Delft/PR" target="blank"> In News. </a> </h3> <br> </center>
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The Novel idea of this project is Antimicrobial peptides (also called host defense peptides) which are an evolutionarily conserved component of the innate immune response and are found among all classes of life. They have been demonstrated to kill Gram negative and Gram positive bacteria</p>
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Latest revision as of 22:37, 28 October 2013


  • Who are we?

    We are the iGEM 2013 team of Delft University of Technology, Netherlands. We are working on the production of antimicrobial peptides in order to kill methicillin resistant Staphylococcus aureus, MRSA.

  • Aim

    To engineer Escherichia coli that can detect MRSA in order to locally produce and deliver antimicrobial peptides. The project is divided into several essential modules.

  • Sensing autoinducing peptides

    The sensing part of the of Staphylococcus aureus Agr system is engineered in E. coli. In that way E. coli detects the AIPs that are produced by Staphylococcus aureus which induces the peptide production.

  • Peptide Production

    E. coli detects Staphylococcus aureus by its own quorum sensing system, and at that moment inactivated antimicrobial peptides are being produced

  • Timer

    With the use of a timer the peptide will be activated by cleavage of an inactivating tag. With the use of a timer, high concentrations of peptide can be delivered very locally in order to efficiently kill MRSA.

  • Kill Switch

    In order to ensure the safety of the system, the peptide activating protease is combined with a kill switch that will kill our E. coli.

  • Zephyr

    Zephyr is a low-cost Do It Yourself (DIY) machine which can scan petridishes and 96 well plates for expression of fluorescence at micrometer scale.

 
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