Team:UFMG Brazil/humanpractice
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As a freshman on iGEM and therefore surrounded by novelty, our team invested on human practices both to let nearby people know about synthetic biology and iGEM, and to reinforce on us what we had come for. And that was hugely rewarding and pretty fun! | As a freshman on iGEM and therefore surrounded by novelty, our team invested on human practices both to let nearby people know about synthetic biology and iGEM, and to reinforce on us what we had come for. And that was hugely rewarding and pretty fun! | ||
- | Basically, we choose these | + | Basically, we choose these approaches for our human practices: |
# Disclose synthetic biology and iGEM in Brazil (in Portuguese language); | # Disclose synthetic biology and iGEM in Brazil (in Portuguese language); | ||
# Talk to experts in the field and with people from other areas and background to seek new perspectives; | # Talk to experts in the field and with people from other areas and background to seek new perspectives; | ||
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Some big questions were always raised during the course of our activities: | Some big questions were always raised during the course of our activities: | ||
- | *Why | + | *Why are we choosing to perform this activity? |
- | *What | + | *What will we learn from this activity? |
- | *How this activity | + | *How will this activity help improving our project? |
=Divulgation= | =Divulgation= | ||
- | [[File:wiki.jpg|200px|thumb|left|No information on wikipedia(pt)]]Synthetic biology is not well known in Brazil and we realized that one of the consequences of this is the small number of teams competing in iGEM. With a little more publicity we could help raise awareness of synthetic biology in Brazil and perhaps increase the number of Brazilian teams in the coming years. | + | [[File:wiki.jpg|200px|thumb|left|No information on wikipedia(pt)]]Synthetic biology is not well known in Brazil and we realized that one of the consequences of this is the small number of brazilian teams competing in iGEM. With a little more publicity we could help raise awareness of synthetic biology in Brazil and perhaps increase the number of Brazilian teams in the coming years. |
A simple example of the lack of information in Portuguese about iGEM: there is no page on wikipedia (of course, we are working to solve this). In this chart below we see the comparison of the interess over time to organic searches on Google by "synthetic biology" and its counterpart in Portuguese. The numbers on the graph reflect how many searches have been done for a particular term, relative to the total number of searches done on Google over time (they don't represent absolute search volume numbers): | A simple example of the lack of information in Portuguese about iGEM: there is no page on wikipedia (of course, we are working to solve this). In this chart below we see the comparison of the interess over time to organic searches on Google by "synthetic biology" and its counterpart in Portuguese. The numbers on the graph reflect how many searches have been done for a particular term, relative to the total number of searches done on Google over time (they don't represent absolute search volume numbers): | ||
<html><center><script type="text/javascript" src="//www.google.com/trends/embed.js?hl=en-US&q=biologia+sint%C3%A9tica,+synthetic+biology&cmpt=q&content=1&cid=TIMESERIES_GRAPH_AVERAGES_CHART&export=5&w=800&h=330"></script></center></html> | <html><center><script type="text/javascript" src="//www.google.com/trends/embed.js?hl=en-US&q=biologia+sint%C3%A9tica,+synthetic+biology&cmpt=q&content=1&cid=TIMESERIES_GRAPH_AVERAGES_CHART&export=5&w=800&h=330"></script></center></html> | ||
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<html><center><a class="twitter-timeline" href="https://twitter.com/iGEMUFMG" data-widget-id="383480863964418048">Tweets de @iGEMUFMG</a> | <html><center><a class="twitter-timeline" href="https://twitter.com/iGEMUFMG" data-widget-id="383480863964418048">Tweets de @iGEMUFMG</a> | ||
<script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}(document,"script","twitter-wjs");</script></center> | <script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}(document,"script","twitter-wjs");</script></center> | ||
+ | </html> | ||
+ | |||
+ | ===SIMI Social Network (Innovation system of the State of Minas Gerais)=== | ||
+ | <html><center><object type="text/html" data="http://www.simi.org.br/comunidade/embed/1882" width="700px" height="590px"></object></center> | ||
</html> | </html> | ||
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To simulate difficulties faced on real experiments, there were incompatibilities among some cards. Promoter and terminator cards were classified according to an arbitrary force from 1 to 5 (represented by the number of full colored stars on cards), suggesting that different sequences present different affinities and, so, act on transcription on different ways. Thus, weak promoters could just be used with strong terminators, and constitutive promoters must join weak terminators following precise indications on each card description. Some options of chassis may apply, but the real possibility of their use should be justified; besides, each chassis must be combined with a specific RBS (bacteria with bacteria RBS, yeast with yeast RBS and so on), pointing the existence of molecular patterns that turn a sequence specific to a certain organism. Finally, the coding region cards included a sort of key genes to solve the problems proposed; the gene originally used by the related iGEM team was our expectation for each mission, but we were open to new creative, wellsupported devices students might present. | To simulate difficulties faced on real experiments, there were incompatibilities among some cards. Promoter and terminator cards were classified according to an arbitrary force from 1 to 5 (represented by the number of full colored stars on cards), suggesting that different sequences present different affinities and, so, act on transcription on different ways. Thus, weak promoters could just be used with strong terminators, and constitutive promoters must join weak terminators following precise indications on each card description. Some options of chassis may apply, but the real possibility of their use should be justified; besides, each chassis must be combined with a specific RBS (bacteria with bacteria RBS, yeast with yeast RBS and so on), pointing the existence of molecular patterns that turn a sequence specific to a certain organism. Finally, the coding region cards included a sort of key genes to solve the problems proposed; the gene originally used by the related iGEM team was our expectation for each mission, but we were open to new creative, wellsupported devices students might present. | ||
- | Download ( | + | Download (English version): <html><a href="https://static.igem.org/mediawiki/2013/7/79/IGEM_BrazilUFMG_Brickard.pdf">https://static.igem.org/mediawiki/2013/7/79/IGEM_BrazilUFMG_Brickard.pdf</a></html> |
<html><center><object width="640" height="480"><param name="movie" value="//www.youtube.com/v/5A55arj4IRA?version=3&hl=en_US"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="//www.youtube.com/v/5A55arj4IRA?version=3&hl=en_US" type="application/x-shockwave-flash" width="640" height="480" allowscriptaccess="always" allowfullscreen="true"></embed></object></center> </html> | <html><center><object width="640" height="480"><param name="movie" value="//www.youtube.com/v/5A55arj4IRA?version=3&hl=en_US"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="//www.youtube.com/v/5A55arj4IRA?version=3&hl=en_US" type="application/x-shockwave-flash" width="640" height="480" allowscriptaccess="always" allowfullscreen="true"></embed></object></center> </html> | ||
- | = | + | =Augmented Reality App= |
<center>[[File:UFMGapp.jpg|600px]]</center> | <center>[[File:UFMGapp.jpg|600px]]</center> | ||
We have created an augmented reality app to allow people to interact with our poster - to be presented at Latin America Jamboree (Chile). In that way they can, using our application, access our videos, charts, graphs, and our wiki page. We think the poster presentations events can be more interesting with this kind of resource and we never saw the use of such technology in academic poster before. | We have created an augmented reality app to allow people to interact with our poster - to be presented at Latin America Jamboree (Chile). In that way they can, using our application, access our videos, charts, graphs, and our wiki page. We think the poster presentations events can be more interesting with this kind of resource and we never saw the use of such technology in academic poster before. | ||
- | The application was created as a skin of the Aurasma system, in a | + | The application was created as a skin of the Aurasma system, in a partnership, and can be downloaded for Android in this website: https://play.google.com/store/apps/details?id=com.aurasma.skinned.ctit&hl=en |
The video below is a demonstration of this app, note that some images of the poster trigger the content (video, image and a 3D model) in the tablet: | The video below is a demonstration of this app, note that some images of the poster trigger the content (video, image and a 3D model) in the tablet: | ||
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=BiOS= | =BiOS= | ||
[[File:BiOS.png|330px|thumb|right|BiOS: website about synbio in Portuguese]] | [[File:BiOS.png|330px|thumb|right|BiOS: website about synbio in Portuguese]] | ||
- | '''BiOS''': this is the name of our website, created to disseminate information in Portuguese about our team, synthetic biology and iGEM. It was set up in February 2013 with introductory information on these subjects and now also has a blog and a forum for questions and answers. | + | '''BiOS''': this is the name of our website, created to disseminate information in Portuguese about our team, synthetic biology and iGEM. It was set up in February 2013 with introductory information on these subjects and, now, also has a blog and a forum for questions and answers. |
To improve users engagement to our site we use the strategy of "gamification", so they earn badges as they participate in the site activity, such as: | To improve users engagement to our site we use the strategy of "gamification", so they earn badges as they participate in the site activity, such as: | ||
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* comment on the blog posts | * comment on the blog posts | ||
* read many discussions | * read many discussions | ||
- | * like a post or a | + | * like a post or a comments |
There are 24 kinds of badges: | There are 24 kinds of badges: | ||
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* 166 posts in the blog (34 of these are about synbio papers) | * 166 posts in the blog (34 of these are about synbio papers) | ||
* 409 pages were indexed by google | * 409 pages were indexed by google | ||
+ | |||
+ | Visit our website: http://bios.ligamedica.com | ||
=Metalab= | =Metalab= | ||
+ | The Meta-Lab aims to be an open space for collaboration, creation, experimentation and development of objects and devices to support teaching and research, through low-tech tools, free knowledge and rapid prototyping machines. Our first goal was to create a site to group DIY projects related to science that could be used by universities and schools in Brazil. | ||
+ | http://metalab.ligamedica.com | ||
==DIY Equipament== | ==DIY Equipament== | ||
- | [[File:prototipo1.jpg| | + | The fluorimeter is a device capable of measuring the intensity of fluorescence emitted by a particular substance, so[[File:prototipo1.jpg|right|300px|thumb|right|First prototype]] we can be be able to know the exact amount of this substance in the sample. The bacteria that is being developed as a diagnostic tool by the UFMG team (CardBio project), will be able to report, according to the fluorescence intensity, the amount of certain substances (biomarkers) in serum of the patient, allowing a more accurate assessment of their health conditions. Despite its simplicity, the fluorometers are expensive equipaments and not so easy to use, so, there is a necessity to develop simple and cheaper model. |
- | [[File:prototipo2.jpg| | + | |
+ | Based on previous iGEM projects[1] we design and build a portable and low cost fluorometer, that could be used with the Cardbio bacteria. The first version is based in the project of the Cambridge Team 2010 (Eglomer), but we use a cheaper light sensor: the TSL235, it´s a light-to-frequency converter that outputs a square wave (50% duty cycle) with frequency directly proportional to light intensity (irradiance). As you can see in the video below, which uses an open hardware oscilloscope to test the sensor: | ||
+ | <center><html><object width="640" height="480"><param name="movie" value="//www.youtube.com/v/sGH6udNYjFM?version=3&hl=en_US"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="//www.youtube.com/v/sGH6udNYjFM?version=3&hl=en_US" type="application/x-shockwave-flash" width="640" height="480" allowscriptaccess="always" allowfullscreen="true"></embed></object></html></center> | ||
+ | In the second version, we inserted a color sensor in the previous project (ADJD-S311, able to discriminate different light frequencies), we remove the keypad and use a larger screen for easier reading. More testing needs to be done to make the device suitable for the needs of the project. | ||
+ | <center>[[File:prototipo3.jpg|400px|thumb|center|The box design]]</center> | ||
+ | <center>[[File:EsquemaUFMG.jpg|400px|thumb|center|Wiring diagram]]</center> | ||
+ | <center>[[File:prototipo2.jpg|400px|thumb|center|Second prototype]]</center> | ||
+ | |||
+ | References [1]: | ||
+ | *https://2012.igem.org/Team:UC_Chile/Arduino | ||
+ | *https://2010.igem.org/Team:Cambridge/Tools/Eglometer | ||
+ | *https://2012.igem.org/Team:TU-Delft/Snifferometer | ||
=Videos= | =Videos= | ||
+ | ==Heart diseases in the Brazilian healthcare system (SUS)== | ||
+ | <html><center><object width="640" height="480"><param name="movie" value="//www.youtube.com/v/GbQtSbW4K5s?version=3&hl=en_US"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="//www.youtube.com/v/GbQtSbW4K5s?version=3&hl=en_US" type="application/x-shockwave-flash" width="640" height="480" allowscriptaccess="always" allowfullscreen="true"></embed></object></center></html> | ||
+ | |||
==Biosafety beyond the laws== | ==Biosafety beyond the laws== | ||
<html><center><object width="640" height="480"><param name="movie" value="//www.youtube.com/v/_vb1huQtiwk?hl=en_US&version=3"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="//www.youtube.com/v/_vb1huQtiwk?hl=en_US&version=3" type="application/x-shockwave-flash" width="640" height="480" allowscriptaccess="always" allowfullscreen="true"></embed></object></center> </html> | <html><center><object width="640" height="480"><param name="movie" value="//www.youtube.com/v/_vb1huQtiwk?hl=en_US&version=3"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="//www.youtube.com/v/_vb1huQtiwk?hl=en_US&version=3" type="application/x-shockwave-flash" width="640" height="480" allowscriptaccess="always" allowfullscreen="true"></embed></object></center> </html> | ||
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==UFMG&Escolas Project== | ==UFMG&Escolas Project== | ||
- | In order to introduce children to synthetic biology universe (literally… virtually travelling to Mars!) of present and future applications, we have created and applied a didactic (and, please… fun!) game for middle school students. This opportunity and public arose from a preceding, very prestigious project (called “UFMG & Escolas”, literally UFMG & Schools; http://www.icb.ufmg.br/biq/ ufmg-escolas/) from the Biological Sciences Institute (ICB) of our university, whose intention is presenting compulsory school students to scientific life. Along a week, students participate in many activities related to what academic community produces. They also have the opportunity of | + | In order to introduce children to synthetic biology universe (literally… virtually travelling to Mars!) of present and future applications, we have created and applied a didactic (and, please… fun!) game for middle school students. This opportunity and public arose from a preceding, very prestigious project (called “UFMG & Escolas”, literally UFMG & Schools; http://www.icb.ufmg.br/biq/ ufmg-escolas/) from the Biological Sciences Institute (ICB) of our university, whose intention is presenting compulsory school students to scientific life. Along a week, students participate in many activities related to what academic community produces. They also have the opportunity of developing and presenting a proposal of an empiric project that they have to idealize and execute. |
- | According to our planning, a short presentation tought | + | According to our planning, a short presentation tought students about the concept of synthetic biology, the biological structures, and the iGEM competition. Once students had this basic information, they were oriented to separate themselves into seven groups, dispersed along the room where there were monitors from iGEM_UFMG team. Each group received a card deck and chose an initially secret mission to explore by engineering a genetically modified organism (GMO). |
On a first moment, the card game (BRICKARD) was played among the group mates: each student should individually try joining the set of cards that he/she judged propitious to form the GMO able to solve the problem presented in the group mission. | On a first moment, the card game (BRICKARD) was played among the group mates: each student should individually try joining the set of cards that he/she judged propitious to form the GMO able to solve the problem presented in the group mission. | ||
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=Art Work= | =Art Work= | ||
- | == | + | ==The E. coli our dilemma== |
<html><center><object width="640" height="480"><param name="movie" value="//www.youtube.com/v/PBvmrv48ZLI?hl=en_US&version=3"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="//www.youtube.com/v/PBvmrv48ZLI?hl=en_US&version=3" type="application/x-shockwave-flash" width="640" height="480" allowscriptaccess="always" allowfullscreen="true"></embed></object></center></html> | <html><center><object width="640" height="480"><param name="movie" value="//www.youtube.com/v/PBvmrv48ZLI?hl=en_US&version=3"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="//www.youtube.com/v/PBvmrv48ZLI?hl=en_US&version=3" type="application/x-shockwave-flash" width="640" height="480" allowscriptaccess="always" allowfullscreen="true"></embed></object></center></html> | ||
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And when it shines I smile, I'll go to Jamboree | And when it shines I smile, I'll go to Jamboree | ||
- | |||
{{Team:UFMG Brazil/sponsor}} | {{Team:UFMG Brazil/sponsor}} |
Latest revision as of 20:35, 27 October 2013