Team:Freiburg

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   <img id="klammer_gross_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
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   <div id="text">  
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   <div id="text1">  
   <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/1#abstract"> Abstract </a> </div>
   <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/1#abstract"> Abstract </a> </div>
   Our Team developed a universal toolkit,  termed uniCAS, that enables customizable gene     
   Our Team developed a universal toolkit,  termed uniCAS, that enables customizable gene     
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     regulation in mammalian cells.  Therefore, we engineered the recently discovered and highly promising   
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     regulation in mammalian cells.  Therefore, we engineered the recently understood and highly promising   
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     CRISPR/Cas9 system.  
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     CRISPR/Cas9 system. <br>
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       The regulation is based on the RNA-guided Cas9 protein, which allows targeting of specific DNA sequences.  
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       The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences.  
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       Our toolkit comprises not only a standardized Cas9 protein,  but also different effector domains for  
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       Our toolkit comprises not only a standardized dCas9 protein,  but also different effector domains for  
       efficient gene activation or repression.  
       efficient gene activation or repression.  
</div>  
</div>  
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<li style="margin-top: -30px; padding-bottom:10px;"><a href="https://2013.igem.org/Team:Freiburg/Project/crrna#multiple_targeting">
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<li style="margin-top: -30px; padding-bottom:10px;"><a href="https://2013.igem.org/Team:Freiburg/Project/crrna">
   <img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png">
   <img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png">
<span> Multiple targeting </span>
<span> Multiple targeting </span>
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   <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
   <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
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       <div id="text_gross" align="left"> Highlights </div>
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       <div id="text_gross" align="left"><a style="position:absolute; top:678px; left:60px; z-index:2" href="https://2013.igem.org/Team:Freiburg/Highlights"> Highlights </div> </a>
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   All about our <br> Project on one <br> Page!
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   <p style="position:absolute; top:720px; left:60px" > All about our <br> project in one <br> slide show! </p>
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  <img src="https://static.igem.org/mediawiki/2013/d/d6/Freiburg_2013_main2_Highlights.png" height="50" style="position:relative; top:-50px; left:110px;"> </div> -->
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   </div>  
   </div>  
   <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">  
   <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">  
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       <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/toolkit">uniTOOL </a></div>
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       <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/toolkit">Manual </a></div>
    
    
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  Use our Toolkit to regulate your genes!
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    Use our Toolkit <br> to regulate your <br> genes!  
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  <!-- <img src="https://static.igem.org/mediawiki/2013/d/d9/Freiburg_2013_main2_Toolkit.png" height="80" style="position:relative; top:-100px; left:50px;"> -->
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    <img src="https://static.igem.org/mediawiki/2013/5/5d/Freiburg_2013_main2_Toolkitnew.png" height="90" style="position:absolute; top:715px; left:464px">  
   </div>  
   </div>  
   </div>
   </div>
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   <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
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   <div id="text-box5">
    
    
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       <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/HumanPractice"> HP </a> </div>
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       <div id="text_gross" align="left"> <a style="position:absolute; top:678px; left:660px; z-index:2" href="https://2013.igem.org/Team:Freiburg/HumanPractice"> Human <br><br> Practice  </a> </div>
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   Our Team developed... </div>  
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   <img src="https://static.igem.org/mediawiki/2013/e/ee/Freibrug_startseite_Humanpractice.png" height="150" style="position:absolute; top:650px; left:655px; z-index:1">
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    </div>  
   <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">  
   <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">  
   </td>
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   <div id="text-box6">  
    
    
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      <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Team2">Team </a></div>
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<div id="text_gross" align="left"> <a style="position:absolute; top:678px; left:910px; z-index:2" href="https://2013.igem.org/Team:Freiburg/News"> News </a></div>
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<p style="padding-top:10px;">
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  Our Team developed... </div>  
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Have a look at our achievements in Lyon & Boston!
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</p>
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<img src="https://static.igem.org/mediawiki/2013/5/56/Best-foundational-advance-2013-freiburg.png" height="130" style="position:absolute; top:600px; left:980px; z-index:1">
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  </div>
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   <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">  
   <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">  
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Latest revision as of 23:52, 9 April 2014


Abstract
Our Team developed a universal toolkit, termed uniCAS, that enables customizable gene regulation in mammalian cells. Therefore, we engineered the recently understood and highly promising CRISPR/Cas9 system.
The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences. Our toolkit comprises not only a standardized dCas9 protein, but also different effector domains for efficient gene activation or repression.
Our Project
Highlights

All about our
project in one
slide show!

Manual
Use our Toolkit
to regulate your
genes!
Human

Practice
News

Have a look at our achievements in Lyon & Boston!

Retrieved from "http://2013.igem.org/Team:Freiburg"