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Team:Paris Saclay/Notebook/August/20
From 2013.igem.org
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| - | Sequencies were good. We | + | Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006. |
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==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ||
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| - | | style="width:250px;border:1px solid black;" | [[]] | + | | style="width:250px;border:1px solid black;" | [[File:Psgel2008.jpg|250px]] |
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*Well 1 : 6µL DNA Ladder | *Well 1 : 6µL DNA Ladder | ||
| - | *Well 2 : 40µL BphR2 Part I+8µl of 6X loading dye | + | *Well 2 : 40µL BphR2 Part I + 8µl of 6X loading dye |
*Gel : 0.8% | *Gel : 0.8% | ||
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| - | We | + | We obtained a frangment at the right size. We can purify it. |
|} | |} | ||
Latest revision as of 01:30, 5 October 2013
Contents |
Notebook : August 20
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006
1 - Sequences analysis
Damir, XiaoJing
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Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006. |
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins
1 - Electrophoresis of PCR products : BphR2 Part I
Nadia
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Expected sizes :
- BphR2 Part I : 178bp
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We obtained a frangment at the right size. We can purify it. |
2 - Gel purification of PCR products : BphR2 Part I
Damir, Nadia
Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
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We lost our fragment. We will do the PCR again. |
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