Team:Leeds

From 2013.igem.org

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{{Team:Leeds_layout|Header=Welcome to the Leeds Wiki!|content=
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<font size="6">[[Team:Leeds/Project|The Microbeagle]]</font>
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==Project Overview==
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We are the Leeds 2013 iGem team, for our project we are hoping to design a modular system to allow for the detection of particles in solution via physical binding. To achieve this we are modifying membrane stress response pathways, utilising fluorescent reporter proteins, using quorum sensing to achieve signal amplification as well as directed evolution to design new attachment peptides.
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Our initial idea is to design a system capable of testing water for the presence of pathogenic bacteria, this system will initially be modeled using silica beads and a Si4 attachment peptide. Our idea is to use Ice Nucleation protein to present the Si4 peptide on the outer membrane of our ''E. coli'' in conjunction with the membrane stress modulator, CpxR promoter, linked to Green Fluorescent protein. The binding of ''E.coli'' to the silica beads will hopefully cause enough membrane stress to induce the CpxR promoter and therefore production of GFP. In addition to this we will be incorporating the LuxI quorum sensing pathway to another GFP tag resulting in massive signal amplification between all of our bacteria in solution creating an all of nothing response to the signal.
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If this is system is successful we can use directed evolution of our peptide tag, presented by INP, to have it attach to pathogenic bacteria resulting in the same GFP response.
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==A biological system designed to detect the presence of pathogens in water samples==
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[[File:Leeds_NEWBS3image.png|400px|right|What can microBeagle do for you? (Additional Image credit to University of Virginia)|link=http://inhabitat.com/6-water-purifying-devices-for-clean-drinking-water-in-the-developing-world/|frameless]]<p align="justify">Micro-Beagle is a novel reporter system for E-coli that, as an iGEM first, has been designed to dynamically detect arbitrary target solids (including other cells) through a mechanism activated by cell surface binding.</p>
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<p align="justify">Micro-Beagle was initially developed to address the need for a low cost, energy efficient and robust tool to be used to assess the effectiveness of current water purification systems. As the Micro-Beagle is intended to operate at a low cost it is ideal for use in low-income areas where the testing of water purification techniques cannot be maintained at an acceptable frequency.</p>
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<p align="justify">Micro-Beagle is designed to also be a modular system, utilising Ice Nucleation Protein to express and position target-binding peptides on the cell surface. Target binding induces membrane stress that activates the Cpx signalling pathway, and Micro-Beagle thus utilises a promoter from this pathway (pCpxR) to initiate expression of a reporter protein, such as GFP.</p>
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<p align="justify">As a proof of concept, we have used silica beads as a model diagnostic target (a pathogen surrogate) and the silica-binding “Si4” sequence as the target-binding peptide.</p>
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<p align="justify">We foresee Micro-Beagle being adapted for both the detection of waterborne pathogens and a variety of other diagnostic applications, and we envision future multisensor Micro-Beagles in which diverse pathogens can be simultaneously and quantitatively measured from a single water sample.</p>
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==The Leeds 2013 iGEM team==
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[[File:Leeds_Map.png|300px|right|The Kings in the North|link=http://maps.google.co.uk/maps?q=Leeds+map&oe=utf-8&client=firefox-a&ie=UTF-8&ei=VHDuUci7J7CT0AWYoYCQDg&ved=0CAoQ_AUoAg|frameless]]
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We are the Leeds 2013 iGEM team, a group of undergrads from various courses at the University of Leeds, all with a passion for synthetic biology!
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<div style="color:red;background:black;tfont-weight:bold;font-size:36pt;text-align:center;padding:19px"> Everything Below Here To Be Removed </div>
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Leeds is a city in West Yorkshire, in the North of England. It has strong historical ties to the wool industry, and is famous for the Tetley brand of bitter ale.
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The University of Leeds is a [http://en.wikipedia.org/wiki/Red_brick_university British Red Brick] and member of the Russell Group and was officially formed as an independent university via Royal Charter from King Edward VII in 1904.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project. Give us background. Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:Leeds | Team Leeds]]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Leeds|Home]]
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!align="center"|[[Team:Leeds/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leeds Official Team Profile]
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!align="center"|[[Team:Leeds/Project|Project]]
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!align="center"|[[Team:Leeds/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Leeds/Modeling|Modeling]]
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!align="center"|[[Team:Leeds/Notebook|Notebook]]
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!align="center"|[[Team:Leeds/Safety|Safety]]
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!align="center"|[[Team:Leeds/Attributions|Attributions]]
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Latest revision as of 03:52, 5 October 2013

Leeds ParkyPanoram.png
 Leeds HomepageHead.png
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Back to iGEM Main Page
Welcome to the Leeds Wiki!
Parky main.png
iGEM Leeds Facebook
iGEM Leeds Twitter
iGEM Leeds Youtube
Leeds socialbanneredge.png
Parky tower.png
awesome looking header


The Microbeagle

A biological system designed to detect the presence of pathogens in water samples

What can microBeagle do for you? (Additional Image credit to University of Virginia)

Micro-Beagle is a novel reporter system for E-coli that, as an iGEM first, has been designed to dynamically detect arbitrary target solids (including other cells) through a mechanism activated by cell surface binding.

Micro-Beagle was initially developed to address the need for a low cost, energy efficient and robust tool to be used to assess the effectiveness of current water purification systems. As the Micro-Beagle is intended to operate at a low cost it is ideal for use in low-income areas where the testing of water purification techniques cannot be maintained at an acceptable frequency.

Micro-Beagle is designed to also be a modular system, utilising Ice Nucleation Protein to express and position target-binding peptides on the cell surface. Target binding induces membrane stress that activates the Cpx signalling pathway, and Micro-Beagle thus utilises a promoter from this pathway (pCpxR) to initiate expression of a reporter protein, such as GFP.

As a proof of concept, we have used silica beads as a model diagnostic target (a pathogen surrogate) and the silica-binding “Si4” sequence as the target-binding peptide.

We foresee Micro-Beagle being adapted for both the detection of waterborne pathogens and a variety of other diagnostic applications, and we envision future multisensor Micro-Beagles in which diverse pathogens can be simultaneously and quantitatively measured from a single water sample.


The Leeds 2013 iGEM team

The Kings in the North

We are the Leeds 2013 iGEM team, a group of undergrads from various courses at the University of Leeds, all with a passion for synthetic biology!
Leeds is a city in West Yorkshire, in the North of England. It has strong historical ties to the wool industry, and is famous for the Tetley brand of bitter ale.
The University of Leeds is a [http://en.wikipedia.org/wiki/Red_brick_university British Red Brick] and member of the Russell Group and was officially formed as an independent university via Royal Charter from King Edward VII in 1904.


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Geneious, our fine sponsors and suppliers of software Bioline, our fine sponsors and suppliers of equipment Qiagen, our fine sponsors and suppliers of PCR kits
Bangs Laboratories, our fine sponsors and suppliers of silica beads
Leeds Homepage
Retrieved from "http://2013.igem.org/Team:Leeds"