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Team:TU-Delft
From 2013.igem.org
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| + | <li> <h2>Aim</h2><div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> To engineer <i>Escherichia coli</i> that can detect MRSA in order to locally produce and deliver antimicrobial peptides. The project is divided into several essential modules. | ||
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<h2>Sensing autoinducing peptides</h2> | <h2>Sensing autoinducing peptides</h2> | ||
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| - | <div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> <i>E.coli</i> detects <i>Staphylococcus aureus</i> by its own quorum sensing system, and at that moment inactivated antimicrobial peptides are being produced </p> | + | <div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> <i>E. coli</i> detects <i>Staphylococcus aureus</i> by its own quorum sensing system, and at that moment inactivated antimicrobial peptides are being produced </p> |
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In order to ensure the safety of the system, the peptide activating protease is combined with a kill switch that will kill our E. coli.</p> | In order to ensure the safety of the system, the peptide activating protease is combined with a kill switch that will kill our E. coli.</p> | ||
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| + | <h2>Zephyr</h2> | ||
| + | <div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> | ||
| + | Zephyr is a low-cost Do It Yourself (DIY) machine which can scan petridishes and 96 well plates for expression of fluorescence at micrometer scale. | ||
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<a href="https://2013.igem.org/Team:TU-Delft/Sensing"> | <a href="https://2013.igem.org/Team:TU-Delft/Sensing"> | ||
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<a href="https://2013.igem.org/Team:TU-Delft/Killswitch"> | <a href="https://2013.igem.org/Team:TU-Delft/Killswitch"> | ||
<img src="https://static.igem.org/mediawiki/2013/f/f8/Kill2.jpg" alt="" HEIGHT="348" WIDTH="600"/> | <img src="https://static.igem.org/mediawiki/2013/f/f8/Kill2.jpg" alt="" HEIGHT="348" WIDTH="600"/> | ||
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<a href="https://2013.igem.org/Team:TU-Delft/Team">1</a> | <a href="https://2013.igem.org/Team:TU-Delft/Team">1</a> | ||
| - | <a href="https://2013.igem.org/Team:TU-Delft/Sensing"> | + | <a href="https://2013.igem.org/Team:TU-Delft/ProjectOverview">2</a> |
| - | <a href="https://2013.igem.org/Team:TU-Delft/Peptides"> | + | <a href="https://2013.igem.org/Team:TU-Delft/Sensing">3</a> |
| - | <a href="https://2013.igem.org/Team:TU-Delft/Timer"> | + | <a href="https://2013.igem.org/Team:TU-Delft/Peptides">4</a> |
| - | <a href="https://2013.igem.org/Team:TU-Delft/Killswitch"> | + | <a href="https://2013.igem.org/Team:TU-Delft/Timer">5</a> |
| + | <a href="https://2013.igem.org/Team:TU-Delft/Killswitch">6</a> | ||
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| - | < | + | <p> <h3> Check out what news in Netherlands has to say about the achievements of our Team </p><a href="https://2013.igem.org/Team:TU-Delft/PR" target="blank"> In News. </a> </h3> <br> </center> |
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Latest revision as of 22:37, 28 October 2013
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Who are we?
We are the iGEM 2013 team of Delft University of Technology, Netherlands. We are working on the production of antimicrobial peptides in order to kill methicillin resistant Staphylococcus aureus, MRSA.
Aim
To engineer Escherichia coli that can detect MRSA in order to locally produce and deliver antimicrobial peptides. The project is divided into several essential modules.
Sensing autoinducing peptides
The sensing part of the of Staphylococcus aureus Agr system is engineered in E. coli. In that way E. coli detects the AIPs that are produced by Staphylococcus aureus which induces the peptide production.
Peptide Production
E. coli detects Staphylococcus aureus by its own quorum sensing system, and at that moment inactivated antimicrobial peptides are being produced
Timer
With the use of a timer the peptide will be activated by cleavage of an inactivating tag. With the use of a timer, high concentrations of peptide can be delivered very locally in order to efficiently kill MRSA.
Kill Switch
In order to ensure the safety of the system, the peptide activating protease is combined with a kill switch that will kill our E. coli.
Zephyr
Zephyr is a low-cost Do It Yourself (DIY) machine which can scan petridishes and 96 well plates for expression of fluorescence at micrometer scale.
Check out what news in Netherlands has to say about the achievements of our Team In News.
Our sponsors
