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Team:INSA Toulouse/contenu/project/biological construction
From 2013.igem.org
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<p class="texte">The first question we had to face for the <i>E.calculus</i> project was the transposition of an electronic device into a reasonable biological system. | <p class="texte">The first question we had to face for the <i>E.calculus</i> project was the transposition of an electronic device into a reasonable biological system. | ||
| - | <br>The diagram of an electronic full adder (see below) can be divided into three independant parts : Input and Output signals (A, B, C<sub>in</sub>, S, C<sub>out</sub>) and logic gates (XOR, AND, OR). The rationale for doing this classification was: logic gates can be universal but input and output signals must be adaptable for diverse applications and microorganisms.</p> | + | <br>The diagram of an electronic full adder (see below) can be divided into three independant parts: Input and Output signals (A, B, C<sub>in</sub>, S, C<sub>out</sub>) and logic gates (XOR, AND, OR). The rationale for doing this classification was: logic gates can be universal but input and output signals must be adaptable for diverse applications and microorganisms.</p> |
| - | <img src="https://static.igem.org/mediawiki/2013/2/2c/Full-adder.png" class=" | + | <img src="https://static.igem.org/mediawiki/2013/2/2c/Full-adder.png" class="imgcenter" /> |
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<h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/input">Input</span></h2> | <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/input">Input</span></h2> | ||
<p class="texte">For the input, we needed a signal that could easily represents "ON" and "OFF" states. Light came as a natural solution because it is easily switchable to "ON" and "OFF" states and color can be varied to represent several inputs (A and B).</a></p> | <p class="texte">For the input, we needed a signal that could easily represents "ON" and "OFF" states. Light came as a natural solution because it is easily switchable to "ON" and "OFF" states and color can be varied to represent several inputs (A and B).</a></p> | ||
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<h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/logic_gates">Logic Gates</span></h2> | <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/logic_gates">Logic Gates</span></h2> | ||
| - | <p class="texte">An electronic full adder is composed of 5 logic gates. Transcriptionally regulated logic gates exist and have already been described. However, a major breakthrough in Synthetic Biology appeared during 2013 with two publications related to recombination based logic gates. They inspired us and are the basis of our work.</a></p> | + | <p class="texte">An electronic full adder is composed of 5 logic gates. Transcriptionally regulated logic gates exist and have already been described. However, a major breakthrough in Synthetic Biology appeared during 2013 with two publications related to recombination-based logic gates. They inspired us and are the basis of our work.</a></p> |
Latest revision as of 16:26, 4 October 2013
Biological Modules
The first question we had to face for the E.calculus project was the transposition of an electronic device into a reasonable biological system.
The diagram of an electronic full adder (see below) can be divided into three independant parts: Input and Output signals (A, B, Cin, S, Cout) and logic gates (XOR, AND, OR). The rationale for doing this classification was: logic gates can be universal but input and output signals must be adaptable for diverse applications and microorganisms.
Input
For the input, we needed a signal that could easily represents "ON" and "OFF" states. Light came as a natural solution because it is easily switchable to "ON" and "OFF" states and color can be varied to represent several inputs (A and B).
Output
The output needed to be a signal that can be easily seen without any complicated device or apparatus, something visual like the color of the organism bearing the full adder.
Carry
The carry (Cin and Cout), belongs to both the input and output modules. We thought of a molecule that could transmit a message from one colony to the other.
Logic Gates
An electronic full adder is composed of 5 logic gates. Transcriptionally regulated logic gates exist and have already been described. However, a major breakthrough in Synthetic Biology appeared during 2013 with two publications related to recombination-based logic gates. They inspired us and are the basis of our work.
Full Adder
The full adder is the integration of the signals (input, ouput, carry) with the logic gates. Its biological description can be found here.
