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Team:Tuebingen/Notebook/Protocols
From 2013.igem.org
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| - | + | <h3>General and <i>E. coli</i> protocols</h3> | |
| - | <h3>General | + | |
<ul> | <ul> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/sob-medium">SOB medium</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/sob-medium">SOB medium</a></li> | ||
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<li><a href="/Team:Tuebingen/Notebook/Protocols/tae">TAE buffer</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/tae">TAE buffer</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/plates">LB plates</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/plates">LB plates</a></li> | ||
| - | <li><a href="/Team:Tuebingen/Notebook/Protocols/ladder-mix">DNA-ladder mix</a></li> | + | <li><a href="/Team:Tuebingen/Notebook/Protocols/ladder-mix">DNA-ladder-buffer mix</a></li> |
<li><a href="/Team:Tuebingen/Notebook/Protocols/gelelectrophoresis">Gelelectrophoresis</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/gelelectrophoresis">Gelelectrophoresis</a></li> | ||
| - | <li><a href="/Team:Tuebingen/Notebook/Protocols/chemo-competentcells">Chemo-competent <i>E. coli</i> cells | + | <li><a href="/Team:Tuebingen/Notebook/Protocols/inoue">Inoue buffer</a></li> |
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/chemo-competentcells">Chemo-competent <i>E. coli</i> cells</li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/trafo"><i>E. coli</i> transformation</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/trafo"><i>E. coli</i> transformation</a></li> | ||
| - | + | <!-- <li><a href="/Team:Tuebingen/Notebook/Protocols/pgem-ligation">pGEM Ligaton - ligation for TA-cloning of PCR products</a></li> --> | |
<li><a href="/Team:Tuebingen/Notebook/Protocols/ligation">Ligation of digested parts and vectors</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/ligation">Ligation of digested parts and vectors</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/glycerol-stocks">Glycerol stocks</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/glycerol-stocks">Glycerol stocks</a></li> | ||
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<li><a href="/Team:Tuebingen/Notebook/Protocols/preparative-restriction">Preparative restriction digest</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/preparative-restriction">Preparative restriction digest</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/control-restriction">Control restriction digest</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/control-restriction">Control restriction digest</a></li> | ||
| - | <li><a href="/Team:Tuebingen/Notebook/Protocols/mini-prep"> | + | <li><a href="/Team:Tuebingen/Notebook/Protocols/mini-prep">Plasmid Preparation</a></li> |
| - | <li><a href="/Team:Tuebingen/Notebook/Protocols/gelextraction"> | + | <li><a href="/Team:Tuebingen/Notebook/Protocols/gelextraction">Gel Extraction</a></li> |
<li><a href="/Team:Tuebingen/Notebook/Protocols/colony-pcr">Colony-PCR</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/colony-pcr">Colony-PCR</a></li> | ||
</ul> | </ul> | ||
| - | |||
| - | <h3><i>Saccharomyces cerevisiae</i> specific protocols</h3> | + | <p><h3><i>Saccharomyces cerevisiae</i>-specific protocols</h3> |
<ul> | <ul> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/ypd">YPD medium</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/ypd">YPD medium</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/sc-dropout">SC DropOut</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/sc-dropout">SC DropOut</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/onestep">ONE-STEP buffer</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/lim">Low Iron Medium (LIM)</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/yeast-trafo">Yeast transformation</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/yeast-trafo">Yeast transformation</a></li> | ||
| - | </ul> | + | <li><a href="/Team:Tuebingen/Notebook/Protocols/tecanreader">Sample preparation and fluorescence measurement using the Tecan Reader</a></li> |
| + | </ul></p> | ||
Latest revision as of 23:36, 15 October 2013
Protocols
General and E. coli protocols
- SOB medium
- LB medium
- TAE buffer
- LB plates
- DNA-ladder-buffer mix
- Gelelectrophoresis
- Inoue buffer
- Chemo-competent E. coli cells
- E. coli transformation
- Ligation of digested parts and vectors
- Glycerol stocks
- PCR
- 3A-Assembly
- Preparative restriction digest
- Control restriction digest
- Plasmid Preparation
- Gel Extraction
- Colony-PCR
Saccharomyces cerevisiae-specific protocols
