"
Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Dailylog
From 2013.igem.org
(Difference between revisions)
| (9 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
{| width="100%" | {| width="100%" | ||
| - | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage July - August Notebook: July 16 - July 31 Daily Log'''</font> | + | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> |
| + | |||
| + | : '''Small Phage July - August Notebook: July 16 - July 31 Daily Log'''</font> | ||
<br> | <br> | ||
| Line 14: | Line 16: | ||
<font color="#333399" size="3" font face="Calibri"> | <font color="#333399" size="3" font face="Calibri"> | ||
| - | : | + | <font size = "4"> |
| + | |||
| + | : <u> '''Small Phage''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | ||
| Line 38: | Line 42: | ||
<font size="4"> '''7/17/13''' </font> | <font size="4"> '''7/17/13''' </font> | ||
| - | - The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for [[Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation]] | + | - The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for [[Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation|7.13 Phage Propagation Experiment]] |
- Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage. | - Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage. | ||
| Line 44: | Line 48: | ||
<br> | <br> | ||
| - | <font size="4"> '''7/ | + | <font size="4"> '''7/19/13''' </font> |
| - | - Performed spot test for | + | - Performed spot test for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.17 Mutagen Concentration Test - Fifth Protocol|7.17 Mutagen Concentration Test - Fifth Protocol]] |
| - | - | + | - Performed titer to determine phage concentration 2 for [[Team:BYU Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13 Phage Propagation|7.13 Phage Propagation Experiment]] |
<br> | <br> | ||
| - | <font size="4"> '''7/ | + | <font size="4"> '''7/22/13''' </font> |
| - | - | + | - A lot of ousekeeping: |
| + | |||
| + | : Went over parental guide, and pre-/post- questions for [[Team:BYU Provo/Outreach/Baxtor|The Adventure of Baxtor Bacteria]] | ||
| + | : Discussed next steps | ||
| + | : Cleanned out fridge and took pictures of plates | ||
| + | : Updated website | ||
<br> | <br> | ||
| - | <font size="4"> '''7/ | + | <font size="4"> '''7/26/13''' </font> |
| - | - | + | - Designed protocols for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]] and decided to conduct an experiment to verify bacteria growth curve (specifics see [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Exp/7.27 Bacteria Growth Curve After Dilution|7.27 Bacteria Growth Curve After Dilution]]) |
| - | - | + | - Started 3mL of BL21 liquid culture overnight. |
<br> | <br> | ||
| - | <font size="4"> '''7/ | + | <font size="4"> '''7/27/13''' </font> |
| - | - | + | - Conducted [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Exp/7.27 Bacteria Growth Curve After Dilution|7.27 Bacteria Growth Curve After Dilution]] |
| - | + | ||
| - | + | ||
<br> | <br> | ||
| - | <font size="4"> '''7/ | + | <font size="4"> '''7/28/13''' </font> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | - Started 15mL of BL21 liquid culture overnight. | |
| - | - Started | + | - Started phage liquid culture to test for superinfection. |
<br> | <br> | ||
| - | <font size="4"> '''7/ | + | <font size="4"> '''7/29/13''' </font> |
| - | - Performed | + | - Performed part 1 of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. |
| - | - | + | - Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.) |
<br> | <br> | ||
| - | <font size="4"> '''7/ | + | <font size="4"> '''7/31/13''' </font> |
| - | - | + | - Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. |
<br> | <br> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
|} | |} | ||
{{TeamBYUProvoFooter}} | {{TeamBYUProvoFooter}} | ||
Latest revision as of 15:35, 9 September 2013
| ||
|
|
7/16/13 - Started approximately 60mL of BL21 liquid culture overnight.
7/17/13 - The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for 7.13 Phage Propagation Experiment - Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.
7/19/13 - Performed spot test for 7.17 Mutagen Concentration Test - Fifth Protocol - Performed titer to determine phage concentration 2 for 7.13 Phage Propagation Experiment
7/22/13 - A lot of ousekeeping:
7/26/13 - Designed protocols for 7.29 Mutagen Concentration Test - Sixth Protocol and decided to conduct an experiment to verify bacteria growth curve (specifics see 7.27 Bacteria Growth Curve After Dilution) - Started 3mL of BL21 liquid culture overnight.
7/27/13 - Conducted 7.27 Bacteria Growth Curve After Dilution
7/28/13 - Started 15mL of BL21 liquid culture overnight. - Started phage liquid culture to test for superinfection.
7/29/13 - Performed part 1 of 7.29 Mutagen Concentration Test - Sixth Protocol. - Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.)
7/31/13 - Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in 7.29 Mutagen Concentration Test - Sixth Protocol.
| |
