Team:BYU Provo/Notebook

From 2013.igem.org

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==March==
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{{TeamBYUProvo}}
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===3/15/13===
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===3/18/13===
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[[File:BYUNotebook.JPG|965px|center|link=]]
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===3/20/13===
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=='''Phage Team'''==
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===3/22/13===
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{| width="100%" style="text-align: top;"
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===3/25/13===
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|- valign="center"
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| style="width: 22%; background-color: transparent;"|
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===3/27/13===
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[[File:BYULPProjectPic.JPG|220px|center|link=]]
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===3/29/13===
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| style="width: 58%; background-color: transparent;"|
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==April==
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<font color="#333399" size="5" font face="Calibri">
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===4/1/13===
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===4/4/13===
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: '''Large Phage Team''' </font>
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===4/5/13===
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<font color="#333399" size="3" font face="Calibri"> 
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===4/8/13===
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: '''Bryan Merrill and Keltzie Westra'''
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===4/10/13===
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: The focus of the Large Phage Team is to make a cross section of phage sizes through the mutagenesis of T4 bacteriophage. Our main area of research is in making T4's already large capsid even larger, while maintaining phenotypic stability. This was done by taking the isolated large phage and observing there later generations to ensure that their phenotype is inherited.
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===4/12/13===
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</font>
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===4/15/13===
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<br>
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==May==
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| style="width: 20%; background-color: transparent;"|
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===5/1/13===
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===5/3/13===
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<br>
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===5/6/13===
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<font color="#333399" size="3" font face="Calibri">
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===5/8/13===
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: [[Team:BYU Provo/Notebook/LargePhage/Winterexp|March-April]]
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===5/10/13===
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: [[Team:BYU Provo/Notebook/LargePhage/Springexp|May-June]]
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===5/13/13===
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: [[Team:BYU Provo/Notebook/LargePhage/Summerexp|July-August]]
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===5/15/13===
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: [[Team:BYU Provo/Notebook/LargePhage/Fallexp|September-October]]
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===5/17/13===
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</font>
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===5/20/13===
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<br>
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===5/22/13===
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|-
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===5/24/13===
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| style="width: 22%; background-color: transparent;"|
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===5/27/13===
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[[File:S4.JPG|220px|center|link=]]
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===5/29/13===
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| style="width: 53%; background-color: transparent;"|
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===5/31/13===
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<font color="#333399" size="5" font face="Calibri">
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: '''Small Phage Team''' </font>
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==June==
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<font color="#333399" size="3" font face="Calibri">
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===6/3/13===
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===6/5/13===
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: '''Xiuqi (Jade) Li and LJ Perry'''
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===6/7/13===
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: We are creating a library of different size T7 bacteriophage capsids. This is accomplished by mutating the phage with a base analog and then isolating the smaller and bigger T7 phages. We are also establishing the relationship between the capsid size and the plaque size of certain phages.
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===6/10/13===
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</font>
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===6/12/13===
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<br>
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===6/14/13===
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| style="width: 25%; background-color: transparent;"|
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===6/17/13===
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<br>
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===6/19/13===
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<font color="#333399" size="3" font face="Calibri">
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===6/21/13===
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: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
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===6/24/13===
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: [[Team:BYU Provo/Notebook/SmallPhage/Springexp|May-June]]
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===6/26/13===
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: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|July-August]]
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===6/28/13===
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: [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|September-October]]
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==July==
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</font>
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===7/1/13===
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===7/3/13===
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<br>
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===7/5/13===
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|-
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===7/8/13===
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| style="width: 22%; background-color: transparent;"|
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===7/10/13===
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[[File:Cesiumchloridegradient.jpg|220px|center|link=]]
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===7/12/13===
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| style="width: 53%; background-color: transparent;"|
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===7/15/13===
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<font color="#333399" size="5" font face="Calibri">
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===7/17/13===
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: '''Phage Purification Team''' </font>
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===7/19/13===
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<font color="#333399" size="3" font face="Calibri"> 
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===7/22/13===
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: '''Amber Brown, Arick Christopher, and Darren Lasko'''
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===7/24/13===
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: As members of the Phage Purification Team, we focus on the purification of bacteriophages and the preparation of ghost capsids. We have worked with several different methods of preparation and can manipulate the procedures to accommodate the type of phage and the capsid size of the mutants.
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===7/26/13===
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</font>
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===7/29/13===
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<br>
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===7/31/13===
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| style="width: 20%; background-color: transparent;"|
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==August==
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<br>
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===8/2/13===
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===8/5/13===
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<font color="#333399" size="3" font face="Calibri">
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===8/7/13===
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: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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===8/9/13===
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: [[Team:BYU Provo/Notebook/Phage_Purification/Springexp|May-June]]
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===8/12/13===
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: [[Team:BYU Provo/Notebook/Phage_Purification/Summerexp|July-August]]
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===8/14/13===
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: [[Team:BYU Provo/Notebook/Phage_Purification/Fallexp|September-October]]
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===8/16/13===
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</font>
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===8/19/13===
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<br>
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===8/21/13===
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|}
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===8/23/13===
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<br>
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===8/26/13===
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=='''Cholera Team'''==
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===8/28/13===
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{| width="100%" style="text-align: top;"
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===8/30/13===
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|- valign="center"
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| style="width: 22%; background-color: transparent;"|
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==September==
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[[File:BYUCCCC.jpg|220px|center|link=]]
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===9/2/13===
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===9/4/13===
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| style="width: 58%; background-color: transparent;"|
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===9/6/13===
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<font color="#333399" size="5" font face="Calibri">
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===9/9/13===
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: '''Cholera-Detection Team''' </font>
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===9/11/13===
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<font color="#333399" size="3" font face="Calibri"> 
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===9/13/13===
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: '''Clarice Harrison, Kendall Kiser, and Kelton Peck'''
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===9/16/13===
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: Our focus is two-fold: 1) develop a system in ''E.coli'' to detect the presence ''V. cholerae'', and 2) integrate ''E.coli'' bacteriophage lambda into a biofilm-degrading machine that is moderated by our detection system. Upon discovering experimentally that bacteriophage lambda can naturally sense cholera, our research efforts have centered on synthetically improving this pathway.
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===9/18/13===
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</font>
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===9/20/13===
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<br>
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===9/23/13===
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| style="width: 20%; background-color: transparent;"|
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===9/25/13===
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<br>
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===9/27/13===
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<font color="#333399" size="3" font face="Calibri">
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===9/30/13===
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: [[Team:BYU Provo/Notebook/Cholera_-_Detection/Winterexp|March-April]]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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: [[Team:BYU Provo/Notebook/Cholera_-_Detection/Springexp|May-June]]
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!align="center"|[[Team:BYU_Provo|Home]]
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!align="center"|[[Team:BYU_Provo/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=BYU_Provo Official Team Profile]
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!align="center"|[[Team:BYU_Provo/Project|Project]]
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!align="center"|[[Team:BYU_Provo/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:BYU_Provo/Modeling|Modeling]]
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!align="center"|[[Team:BYU_Provo/Notebook|Notebook]]
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!align="center"|[[Team:BYU_Provo/Safety|Safety]]
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!align="center"|[[Team:BYU_Provo/Attributions|Attributions]]
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|}
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: [[Team:BYU Provo/Notebook/Cholera_-_Detection/Summerexp|July-August]]
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: [[Team:BYU Provo/Notebook/Cholera_-_Detection/Fallexp|September-October]]
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</font>
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<br>
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|-
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| style="width: 22%; background-color: transparent;"|
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[[File:BYU2013-CholeraGroup1.jpg|220px|center|link=]]
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| style="width: 53%; background-color: transparent;"|
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<font color="#333399" size="5" font face="Calibri">
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: '''Cholera-Enzyme Team''' </font>
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<font color="#333399" size="3" font face="Calibri"> 
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: '''Nathan Sabin and Michael Schellhous'''
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: On the Cholera-Enzyme team our focus is researching enzymes that will degrade the ''V. cholerae'' biofilm. Our goal is to find enzymes that have potential to degrade the biofilm, isolate them, clone them into ''E. coli'', and design an assay to test their biofilm-degrading ability.
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</font>
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<br>
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| style="width: 25%; background-color: transparent;"|
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<br>
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<font color="#333399" size="3" font face="Calibri">
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: [[Team:BYU Provo/Notebook/Cholera - Enzymes/March-April|March-April]]
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: [[Team:BYU Provo/Notebook/Cholera - Enzymes/May-June|May-June]]
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: [[Team:BYU Provo/Notebook/Cholera - Enzymes/July-August|July-August]]
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: [[Team:BYU Provo/Notebook/Cholera - Enzymes/September-October|September-October]]
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</font>
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<br>
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|}
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<br> <br>
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
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{{TeamBYUProvoFooter}}

Latest revision as of 03:49, 28 September 2013

BYUNotebook.JPG

Phage Team

BYULPProjectPic.JPG

Large Phage Team

Bryan Merrill and Keltzie Westra
The focus of the Large Phage Team is to make a cross section of phage sizes through the mutagenesis of T4 bacteriophage. Our main area of research is in making T4's already large capsid even larger, while maintaining phenotypic stability. This was done by taking the isolated large phage and observing there later generations to ensure that their phenotype is inherited.



March-April
May-June
July-August
September-October


S4.JPG

Small Phage Team

Xiuqi (Jade) Li and LJ Perry
We are creating a library of different size T7 bacteriophage capsids. This is accomplished by mutating the phage with a base analog and then isolating the smaller and bigger T7 phages. We are also establishing the relationship between the capsid size and the plaque size of certain phages.



March-April
May-June
July-August
September-October


Cesiumchloridegradient.jpg

Phage Purification Team

Amber Brown, Arick Christopher, and Darren Lasko
As members of the Phage Purification Team, we focus on the purification of bacteriophages and the preparation of ghost capsids. We have worked with several different methods of preparation and can manipulate the procedures to accommodate the type of phage and the capsid size of the mutants.



March-April
May-June
July-August
September-October



Cholera Team

BYUCCCC.jpg

Cholera-Detection Team

Clarice Harrison, Kendall Kiser, and Kelton Peck
Our focus is two-fold: 1) develop a system in E.coli to detect the presence V. cholerae, and 2) integrate E.coli bacteriophage lambda into a biofilm-degrading machine that is moderated by our detection system. Upon discovering experimentally that bacteriophage lambda can naturally sense cholera, our research efforts have centered on synthetically improving this pathway.



March-April
May-June
July-August
September-October


BYU2013-CholeraGroup1.jpg

Cholera-Enzyme Team

Nathan Sabin and Michael Schellhous
On the Cholera-Enzyme team our focus is researching enzymes that will degrade the V. cholerae biofilm. Our goal is to find enzymes that have potential to degrade the biofilm, isolate them, clone them into E. coli, and design an assay to test their biofilm-degrading ability.



March-April
May-June
July-August
September-October





Retrieved from "http://2013.igem.org/Team:BYU_Provo/Notebook"