Team:USTC CHINA

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        <li class="active"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs of Immune Experiments</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice">Human Practice</a>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" >Communication</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Activity">Activity</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Team</a>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Members</a></li>
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                  <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></li>
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                  <li><a href="https://igem.org/2013_Judging_Form?id=1074#iGEM_Medals">Achievements</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
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        <h1>In Situ <span>T</span>ransdermal <span>Vaccine</span></h1>
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        <p>2013, We Redefined Vaccine<br />A Revolutionary Transdermal Vaccine<br />Safe, Reliable, Needle-free and Available for Everyone<br />
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            <a class="button-home" href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview"><span>Project </span></a>
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<h1>Motivation</h1>
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  We hope to develop a fresh-expression transdermal vaccination.
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  <br />We hope everyone in this world could equally enjoy the benefit of medical progress.<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">more</a>
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<h1>Design of Project</h1>
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  With the support of <span><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background#" target="_blank">TD-1</a></span>, a special polypeptide which can greatly facilitate macromolecule transdermal delivery across intact skin, we constructed three different fuctional engineered <i>Bacillus subtilis</i>; besides, we designed a reporter system, including a kill switch, in the fouth type <i>B.subtilis</i> to improve usability and ensure biosafety.</div>
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  <span>ANTIGEN</span><br />
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As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will make up great percentage of<br />in the total bacteria in our "band-aid".
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<li><a href="#"><span>overview</span></a></li>
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  <span>LTB</span><br />
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As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a widely used adjuvant for most of exsiting injected vaccine.
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
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  <span>TNFα</span><br />
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TNFα is a chemotactic factor recruiting Langerhans Cells, the antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for collecting LC and triggering more intense immune responses.
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  <span>REPORTER</span><br />
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Reporter notifies users the status of vaccine patch and when to stick.  Furthermore, it contains killing switch as a biosafety guard.
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<h1>Experimental Measurement</h1>
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<span>Construction of Vectors</span><br />
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We took <i>E.coli</i> and the pET vector as the positive control and realized our ideas in the <div class="oblique">B.subtilis</div> expression system based on pHT43 shuttle vector.<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a>
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  <span>Tests on fusion protein</span><br />
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  To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verify our design.<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a>
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  <span>Immunogenicity experiments</span><br />
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  The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results/FurthurWork">more</a>
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<h1>Modeling</h1>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Suicide mechanism of the reporter system</a></h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">The optimal culture conditions of Bacillus subtilis</a></h2>
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                <span style="display: none;" class="bottom"><strong>Our pipettes</strong><br>
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Designs of Immune Experiments</a></h2>
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<div id="content1">
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<div id="hum-bar">
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<p>project</p></div>
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<h1>Human Practice</h1>
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<div id="hum-col">
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication">
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Communication</a></h2>
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<a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication"><img src="https://static.igem.org/mediawiki/2013/5/5e/2013ustc-china_China-map.png" width="310" height="259" /></a>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication">
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Activity</a></h2>
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</a>
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<div  id="sponfra">
 
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<div id="spo">thanks our sponsors</div>
 
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<div class="subspon" align="center"><img src="Ustcif.jpg" width="106" height="47" /></div>
 
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<div class="subspon" align="center"><img src="GRADUATE.png" width="106" height="47" /></div>
 
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<div class="subspon" align="center"><img src="20120830175936Life.jpg" width="106" height="47"/></div>
 
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<div class="subspon" align="center"><img src="Phy.png" width="106" height="47" /> </div>
 
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<div class="subspon" align="center"><img src="Teaching.jpg" width="106" height="47" /></div>
 
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</div></div>
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<div id="footer">
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<div id="thanks">
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    <h3 id="sponsors-title" align="center"><em>Thanks to the sponsors</em></h3><br>
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    <div class="sponsor" id="db">
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      <p align="center">Deutsche Bank, China</p>
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        <div align="center"><a href="https://china.db.com/index_e.html" target="_blank"><img src="https://static.igem.org/mediawiki/2013/a/a7/2013ustc-china_Db-logo.png" alt="Deutsche Bank, China" height="65px" align="absmiddle" width="280px"></a>
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        </div>
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    </div>
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    <div class="sponsor">
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        <p align="center">School of Life Sciences, USTC</p>
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        <div align="center"><a href="http://en.biox.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/9/98/2013ustc-china_Life-logo.png" alt="School of Life Sciences, USTC" height="65px" align="absmiddle" width="280px"></a>
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        </div>
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    </div>
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    <div class="sponsor">
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      <p align="center">USTC<span style="color:#fcc644">IF</span></p>
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        <div align="center"><a href="http://www.ustcif.org/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/3/3c/2013ustc-china_Ustcif-logo.png" height="65px" align="absmiddle" width="280px"></a>
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        </div>
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    </div>
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    <div class="sponsor">
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        <p align="center">School of the Gifted Young, USTC</p>
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        <div align="center"><a href="http://en.scgy.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/c/c1/2013ustc-china_Gifted_young.jpg" height="65px" align="absmiddle" width="280px"></a>
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        </div>
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    </div>
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    <div class="sponsor">
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        <p align="center">School of physics, USTC</p>
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        <div align="center"><a href="http://en.physics.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/6/68/2013ustc-china_Physics-logo.png" height="65px" align="absmiddle" width="280px"></a>
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        </div>
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    </div>
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    <div class="sponsor">
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        <p align="center">Teaching Affairs Office of USTC</p>
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        <div align="center"><a href="http://www.teach.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/f/f9/2013ustc-china_Teaching-logo.png" alt="Teaching Affairs Office Of USTC" style="background-color:white" height="65px" align="absmiddle" width="280px"></a>
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        </div>
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    </div>
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Latest revision as of 03:28, 29 October 2013

USTC_China

In Situ Transdermal Vaccine

2013, We Redefined Vaccine
A Revolutionary Transdermal Vaccine
Safe, Reliable, Needle-free and Available for Everyone

Project

Motivation

We hope to develop a fresh-expression transdermal vaccination.
We hope everyone in this world could equally enjoy the benefit of medical progress.
more

Design of Project

With the support of TD-1, a special polypeptide which can greatly facilitate macromolecule transdermal delivery across intact skin, we constructed three different fuctional engineered Bacillus subtilis; besides, we designed a reporter system, including a kill switch, in the fouth type B.subtilis to improve usability and ensure biosafety.
ANTIGEN
As the core of our in-situ system,
TD1-antigen expresser is designed
and will make up great percentage of
in the total bacteria in our "band-aid".
more
LTB
As one kind of our adjuvant expresser,
TD1-LTB is used to enhance the antigenicity.
For LTB has so many advantages, it's a widely used adjuvant for most of exsiting injected vaccine.
more
TNFα
TNFα is a chemotactic factor recruiting Langerhans Cells, the antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for collecting LC and triggering more intense immune responses.
more
REPORTER
Reporter notifies users the status of vaccine patch and when to stick. Furthermore, it contains killing switch as a biosafety guard.
more

Experimental Measurement

Construction of Vectors
We took E.coli and the pET vector as the positive control and realized our ideas in the
B.subtilis
expression system based on pHT43 shuttle vector.
more
Tests on fusion protein
To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verify our design.
more
Immunogenicity experiments
The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments
more

Retrieved from "http://2013.igem.org/Team:USTC_CHINA"