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16/08/13
From 2013.igem.org
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(Created page with "==Running the gel from sonication series== *Samples were taken from sonicated tube every 30min intervals, a total of 6 samples. *Each was diluted 100 fold to get a concentration ...") |
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| + | <div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;"> | ||
| + | {| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center" | ||
| + | !align="center"|[[Team:Leicester|Home]] | ||
| + | !align="center"|[[Team:Leicester/Team|Team]] | ||
| + | !align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile] | ||
| + | !align="center"|[[Team:Leicester/Project|Project]] | ||
| + | !align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]] | ||
| + | !align="center"|[[Team:Leicester/Modeling|Modeling]] | ||
| + | !align="center"|[[Team:Leicester/Notebook|Notebook]] | ||
| + | !align="center"|[[Team:Leicester/Safety|Safety]] | ||
| + | !align="center"|[[Team:Leicester/Attributions|Attributions]] | ||
| + | |} | ||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
==Running the gel from sonication series== | ==Running the gel from sonication series== | ||
*Samples were taken from sonicated tube every 30min intervals, a total of 6 samples. | *Samples were taken from sonicated tube every 30min intervals, a total of 6 samples. | ||
*Each was diluted 100 fold to get a concentration in the range of 40-50ng/ul | *Each was diluted 100 fold to get a concentration in the range of 40-50ng/ul | ||
*5ul of DNA was taken from the dilution, to which 10ul of sterile water and 5ul of orange G was added | *5ul of DNA was taken from the dilution, to which 10ul of sterile water and 5ul of orange G was added | ||
| - | *Samples were run against a 1kb ladder and control herring sperm DNA, which was not sonicated | + | *Samples were run against a 1kb ladder and control herring sperm DNA, which was not sonicated. |
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| - | + | ||
Latest revision as of 09:18, 22 August 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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Running the gel from sonication series
- Samples were taken from sonicated tube every 30min intervals, a total of 6 samples.
- Each was diluted 100 fold to get a concentration in the range of 40-50ng/ul
- 5ul of DNA was taken from the dilution, to which 10ul of sterile water and 5ul of orange G was added
- Samples were run against a 1kb ladder and control herring sperm DNA, which was not sonicated.
