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19/08/13
From 2013.igem.org
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(→Running both denatured and sonicated samples on gel) |
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| + | {| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center" | ||
| + | !align="center"|[[Team:Leicester|Home]] | ||
| + | !align="center"|[[Team:Leicester/Team|Team]] | ||
| + | !align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile] | ||
| + | !align="center"|[[Team:Leicester/Project|Project]] | ||
| + | !align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]] | ||
| + | !align="center"|[[Team:Leicester/Modeling|Modeling]] | ||
| + | !align="center"|[[Team:Leicester/Notebook|Notebook]] | ||
| + | !align="center"|[[Team:Leicester/Safety|Safety]] | ||
| + | !align="center"|[[Team:Leicester/Attributions|Attributions]] | ||
| + | |} | ||
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==Denaturing sonicated DNA== | ==Denaturing sonicated DNA== | ||
*Denaturing DNA by heat shock for 10mins in a 95C heat block | *Denaturing DNA by heat shock for 10mins in a 95C heat block | ||
Latest revision as of 09:17, 22 August 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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Denaturing sonicated DNA
- Denaturing DNA by heat shock for 10mins in a 95C heat block
- Adding 5ul of OG dye
Running both denatured and sonicated samples on gel
- For just the sonication samples 1-6, 5ul of OG and 10ul of sterile water was added to 5ul of DNA.
- 5ul of 1kb ladder was used for 3 wells each.
- The last 6 wells after ladder are the denatured DNA samples.
- We were expecting to see fragmentation for the denatured samples.
- There is no significant difference in between the sonicated samples and the sonicated and denatured ones.
- There is a slight decrease in bands depending on the sonication time interval.
- Suggestions are to use ultra-sonification to view the effects focused just on the sample.
