Team:BYU Provo/Notebook/LargePhage/Springexp

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(Difference between revisions)

Latest revision as of 21:33, 26 September 2013

Large Phage May - June Notebook
Large Phage March-April May-June July-August September-October	May 1 - May 12 30 second UV test! Daily log
	May 13 - May 26 45 and 90 Second UV test Daily log
	May 27 - June 9 Preparing for phage amplification and mutagenesis Daily log Experiment Listing Progress Report
	June 10 - June 30 Mutagenesis, experiments with our switch to agarose. Daily log

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 {| width="100%"
-| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Large Phage May - June Notebook'''</font>
+| colspan="3" | <font color="#333399" size="5" font face="Calibri">
+: '''Large Phage May - June Notebook'''</font>
 <br>
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 <font color="#333399" size="3" font face="Calibri">
-: [[Team:BYU_Provo/Large_Phage|Overview]]
+<font size = "4">
+: <u> '''Large Phage''' </u> </font>
 : [[Team:BYU Provo/Notebook/LargePhage/Winterexp|March-April]]
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 <font size="5" font face="Calibri"> '''May 1 - May 12''' </font>
 <br>
-<font size="3" font face="Calibri"> This is the start of Spring Term at BYU! The weather is finally getting better: no more snowing. And we are ready to do some serious lab work! While we are waiting for our mutgen, 5-bromodeoxyuridine, to arrive, we decided to start this term with establishing the basic procedures for our direct evolution method. Specifically, this involves making sure that we can (1) grow and amplify T7 phage in liquid culture, (2) select for smaller phage using concentrated top agar, and (3) amplify phage from a single plaque. During these two weeks, we also designed primers for cloning and sequencing T7 capsid proteins as well as mapped out detailed procedures for both cloning T7 capsid proteins and applying mutagen. </font>
+<font size="3" font face="Calibri"> 30 second UV test!</font>
 <br>
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 [[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/Explist|Experiment Listing]]
-[[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/PR| Progress Report]] </font>
 <br>
-| style="width: 20%; background-color: transparent;"| [[File:Spring1.JPG|250px|center]]
+| style="width: 20%; background-color: transparent;"| [[File:pretty1.JPG|200px|center]]
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 <br>
-<font size="3" font face="Calibri"> In these two weeks, we performed our first round of mutagenesis and first PCR. Although we are still testing the results to optimize our protocol, we are optimistic about the outcome!</font>
+<font size="3" font face="Calibri"> 45 and 90 Second UV test </font>
 <br>
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 [[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period2/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period2/Explist|Experiment Listing]]
+| style="width: 20%; background-color: transparent;"| [[File:pretty2.JPG|200px|center]]
-[[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period2/PR| Progress Report]] </font>
-<br>
-| style="width: 20%; background-color: transparent;"| [[File:Spring2.JPG|250px|center]]
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 <br>
-<font size="3" font face="Calibri"> Add description! </font>
+<font size="3" font face="Calibri">Preparing for phage amplification and mutagenesis</font>
 <br>
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-| style="width: 20%; background-color: transparent;"| [[File:Spring3.JPG|250px|center]]
+| style="width: 20%; background-color: transparent;"| [[File:pretty3.JPG|200px|center]]
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 | style="width: 18%; background-color: transparent;"|
-| <font size="5" font face="Calibri"> '''June 10 - June 23''' </font>
+| <font size="5" font face="Calibri"> '''June 10 - June 30''' </font>
 <br>
-<font size="3" font face="Calibri"> Add description! </font>
+<font size="3" font face="Calibri"> Mutagenesis, experiments with our switch to agarose. </font>
 <br>
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 [[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period4/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period4/Explist|Experiment Listing]]
-[[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period4/PR| Progress Report]] </font>
 <br>
-| style="width: 20%; background-color: transparent;"| [[File:Spring4.JPG|250px|center]]
+| style="width: 20%; background-color: transparent;"| [[File:Pretty4.JPG|200px|center]]
 |-
 | style="width: 18%; background-color: transparent;"|
-| <font size="5" font face="Calibri"> '''June 24 - June 30''' </font>
-<br>
-<font size="3" font face="Calibri"> Add description! </font>
-<br>
-<font color="#333399" size="4" font face="Calibri">
-[[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period5/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period5/Explist|Experiment Listing]]
-[[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period5/PR| Progress Report]] </font>
-<br>
-| style="width: 20%; background-color: transparent;"| [[File:Winter2.JPG|250px|center]]