Team:Paris Saclay/Notebook/July/15

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{{Team:Paris_Saclay/incl_debut_generique}}
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='''Notebook : July 15'''=
='''Notebook : July 15'''=
=='''Lab work'''==
=='''Lab work'''==
-
constructing
 
-
<br>
+
==='''A - Aerobic/Anaerobic regulation system'''===
 +
 
 +
===='''Objective : obtaining BBa_K1155000'''====
 +
 
 +
===='''1 - Sequensis analysis of BBa_K1155000'''====
 +
 
 +
Abdou
 +
 
 +
{|
 +
| style="border:1px solid black;padding:5px;background-color:#DE;" |
 +
WE HAVE OUR FIRST BIOBRICK !
 +
The sequence analysis was good. We call our biobrick BBa_K1155000.
 +
|}
 +
 
 +
===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''====
 +
 
 +
===='''1 - Liquid culture of BBa_B0015, BBa_B0017, BBa_I732019'''====
 +
 
 +
Sheng, Zhou
 +
 
 +
{|
 +
| style="border:1px solid black;padding:5px;background-color:#DE;" |
 +
Tranformations of 07/12/13 works. We will do a liquid culture of them.
 +
|}
 +
 
 +
We used 5 colonies of each plasmid.
 +
 
 +
 
 +
==='''B - PCB sensor system'''===
 +
 
 +
===='''Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2'''====
 +
 
 +
===='''1 - Digestion of BBa_K1155001, BBa_K1155002 and BphR2 in pSB1C3 by EcoRI/PstI and SacII to check the size of fragments'''====
 +
 
 +
Abdou
 +
 
 +
Used quantities :
 +
 
 +
* EcoRI/PstI :
 +
** DNA : 8µL
 +
** Buffer Orange : 1.6µL
 +
** EcoRI : 1µL
 +
** PstI : 1µL
 +
** H2O : 4.4µL
 +
 
 +
* SacII :
 +
** DNA : 8µL
 +
** Buffer Blue : 1.6µL
 +
** SacII : 1µL
 +
** H2O : 5.4µL
 +
 
 +
We let the digestion 1h30 at 37°C.
 +
 
 +
===='''2 - PCR of digestion of BBa_K1155001, BBa_K1155002 and BphR2 in pSB1C3'''====
 +
 
 +
Anaïs, Sheng, Zhou
 +
 
 +
Used quantities :
 +
 
 +
We mix our colonies in 20µL of H2O :
 +
 
 +
Used quantities :
 +
* BphA1 or BphR1 or BphR2 : 2µL
 +
* Mix : (it was divided in 2 tubes for each oligo combinaison with 23µL of mix in each tube)
 +
** VF or Pfnr_Up : 6µL
 +
** VR or Pfnr_Down or VR : 6µL
 +
** dNTP : 6µL
 +
** Buffer Dream Taq : 30µL
 +
** Dream Taq : 6µL
 +
** H2O : 246µL
 +
 
 +
PCR Program :
 +
 
 +
* BphR1 :
 +
 
 +
[[File:PsPCRR11507.jpg|400px]]
 +
 
 +
* BphA1, BphR2 :
 +
 
 +
[[File:PsPCRR2A11507.jpg|400px]]
 +
 
{| border="1" align="center"
{| border="1" align="center"
|[[Team:Paris Saclay/Notebook/July/12|<big>Previous week</big>]]
|[[Team:Paris Saclay/Notebook/July/12|<big>Previous week</big>]]
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|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
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|[[Team:Paris Saclay/Notebook/July/16|<big>next day</big>]]
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|[[Team:Paris Saclay/Notebook/July/16|<big>Next day</big>]]
|}
|}
{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Latest revision as of 23:59, 4 October 2013

Navigation Home Team Project Labwork Notebook Human practices

Contents

Notebook : July 15

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155000

1 - Sequensis analysis of BBa_K1155000

Abdou

WE HAVE OUR FIRST BIOBRICK ! The sequence analysis was good. We call our biobrick BBa_K1155000.

Objective : obtaining BBa_K1155003, BBa_K1155007

1 - Liquid culture of BBa_B0015, BBa_B0017, BBa_I732019

Sheng, Zhou

Tranformations of 07/12/13 works. We will do a liquid culture of them.

We used 5 colonies of each plasmid.


B - PCB sensor system

Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2

1 - Digestion of BBa_K1155001, BBa_K1155002 and BphR2 in pSB1C3 by EcoRI/PstI and SacII to check the size of fragments

Abdou

Used quantities :

  • EcoRI/PstI :
    • DNA : 8µL
    • Buffer Orange : 1.6µL
    • EcoRI : 1µL
    • PstI : 1µL
    • H2O : 4.4µL
  • SacII :
    • DNA : 8µL
    • Buffer Blue : 1.6µL
    • SacII : 1µL
    • H2O : 5.4µL

We let the digestion 1h30 at 37°C.

2 - PCR of digestion of BBa_K1155001, BBa_K1155002 and BphR2 in pSB1C3

Anaïs, Sheng, Zhou

Used quantities :

We mix our colonies in 20µL of H2O :

Used quantities :

  • BphA1 or BphR1 or BphR2 : 2µL
  • Mix : (it was divided in 2 tubes for each oligo combinaison with 23µL of mix in each tube)
    • VF or Pfnr_Up : 6µL
    • VR or Pfnr_Down or VR : 6µL
    • dNTP : 6µL
    • Buffer Dream Taq : 30µL
    • Dream Taq : 6µL
    • H2O : 246µL

PCR Program :

  • BphR1 :

PsPCRR11507.jpg

  • BphA1, BphR2 :

PsPCRR2A11507.jpg

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