Team:Freiburg

From 2013.igem.org

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ABSTRACT
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  <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/1#abstract"> Abstract </a> </div>
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/*
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  Our Team developed a universal toolkit, termed uniCAS, that enables customizable gene   
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  * jQuery FlexSlider v2.0
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    regulation in mammalian cellsTherefore, we engineered the recently understood and highly promising  
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* http://www.woothemes.com/flexslider/
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    CRISPR/Cas9 system. <br>
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  *
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      The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences.  
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* Copyright 2012, Julia Voß
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      Our toolkit comprises not only a standardized dCas9 protein, but also different effector domains for
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  * Free to use under the MIT license.
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      efficient gene activation or repression.
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* http://www.opensource.org/licenses/mit-license.php
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PROJECT
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<div id="text-box2">
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    <div id="text_gross" align="left"><a href="https://2013.igem.org/Team:Freiburg/Project/1"> Our Project </a></div>
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<span> Activation </span>
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<span> Repression </span>
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<img  src="https://static.igem.org/mediawiki/2013/e/e7/Repression_freiburg_13.png" style="height:55px;">
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</a>
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<span> Epigenetics </span>
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<li style="margin-top: -30px; padding-bottom:10px;"><a href="https://2013.igem.org/Team:Freiburg/Project/crrna">
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  <img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png">
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<span> uniBAss</span>
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</a>
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  <img src="https://static.igem.org/mediawiki/2013/9/9a/Freiburg_2013_main2_Licht.png" style="height:57px;">
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<span> Light induction </span>
 +
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 +
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TABLE UNTEN
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HIGHLIGHTS
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  <td width="300px">
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 +
  <div id="text-box3">  
 +
      <div id="text_gross" align="left"><a style="position:absolute; top:678px; left:60px; z-index:2" href="https://2013.igem.org/Team:Freiburg/Highlights"> Highlights </div> </a>
 +
  <p style="position:absolute; top:720px; left:60px" > All about our <br> project in one <br> slide show! </p>
 +
<img src="https://static.igem.org/mediawiki/2013/0/0e/Freiburg_2013_main2_Highlights5.png" height="190" style="position:absolute; top:614px; left:105px; z-index:1">
 +
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 +
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 +
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                        <img src="https://static.igem.org/mediawiki/2013/d/d2/Project_kachel.png">
 
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                            <div class="slide-heading">
 
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                                Our Toolbox
 
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                            </div>
 
-
                         
 
-
all what you need
 
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                            <p><a href="https://2013.igem.org/Team:Freiburg/Project/1">
 
-
                            Click here to read more</a> about our project
 
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                            </p>
 
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                        </div>
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<!-- ########################################################################################
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                <li>
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                    <div class="frontpage-slide">
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                        <img src="">
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                        <div class="slide-right-col">
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                            <div class="slide-heading">
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                              I do not know
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                            </div>
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-
+
TOOLKIT
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                            <p><a href="https://2013.igem.org/Team:Freiburg/Project/1">
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                            Click here to read more </a> about our methods...
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                          </p>
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                        </div>
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                        <img src="https://static.igem.org/mediawiki/2013/d/d2/Project_kachel.png">
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                        <div class="slide-right-col">
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                            <div class="slide-heading">
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                                The Potential
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                            </div>
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+
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                            <p><a href="http://">
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                            Click here to read more</a> </p>                     
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                        </div>
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######################################################################################## -->
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                                Light-Activation
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                            </div>
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                            <p><a href="https://2013.igem.org/Team:Freiburg/Project/1">
 
-
                            Click here to read more</a>
 
-
                          </p>
 
 +
  <td width="300px">
 +
  <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
 +
  <div id="text-box4">
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      <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/toolkit">Manual </a></div>
 +
 
 +
    Use our Toolkit <br> to regulate your <br> genes!
 +
    <img src="https://static.igem.org/mediawiki/2013/5/5d/Freiburg_2013_main2_Toolkitnew.png" height="90" style="position:absolute; top:715px; left:464px">
 +
  </div>
 +
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HUMAN PRACTICE
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  <div id="text-box5">
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      <div id="text_gross" align="left"> <a style="position:absolute; top:678px; left:660px; z-index:2" href="https://2013.igem.org/Team:Freiburg/HumanPractice"> Human <br><br> Practice  </a> </div>
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<div id="tour">
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  <img src="https://static.igem.org/mediawiki/2013/e/ee/Freibrug_startseite_Humanpractice.png" height="150" style="position:absolute; top:650px; left:655px; z-index:1">  
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<img src="https://static.igem.org/mediawiki/2013/b/bc/Cas9_tour.png">
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TEAM
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<img src="https://static.igem.org/mediawiki/2013/f/fb/Team_kachel.png">
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  <td width="250px">
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  <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
 +
  <div id="text-box6">
 +
 
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<div id="text_gross" align="left"> <a style="position:absolute; top:678px; left:910px; z-index:2" href="https://2013.igem.org/Team:Freiburg/News"> News  </a></div>
 +
<p style="padding-top:10px;">
 +
Have a look at our achievements in Lyon & Boston!
 +
</p>
 +
<img src="https://static.igem.org/mediawiki/2013/5/56/Best-foundational-advance-2013-freiburg.png" height="130" style="position:absolute; top:600px; left:980px; z-index:1">
 +
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 +
  <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
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  </td>
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Latest revision as of 23:52, 9 April 2014


Our Team developed a universal toolkit, termed uniCAS, that enables customizable gene regulation in mammalian cells. Therefore, we engineered the recently understood and highly promising CRISPR/Cas9 system.
The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences. Our toolkit comprises not only a standardized dCas9 protein, but also different effector domains for efficient gene activation or repression.

All about our
project in one
slide show!

Use our Toolkit
to regulate your
genes!

Have a look at our achievements in Lyon & Boston!