Team:Freiburg

From 2013.igem.org

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*
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ABSTRACT
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  <img id="klammer_gross_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
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  <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/1#abstract"> Abstract </a> </div>
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 +
  Our Team developed a universal toolkit,  termed uniCAS, that enables customizable gene   
 +
    regulation in mammalian cells.  Therefore, we engineered the recently understood and highly promising 
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    CRISPR/Cas9 system. <br>
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      The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences.
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      Our toolkit comprises not only a standardized dCas9 protein,  but also different effector domains for
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      efficient gene activation or repression.
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</div>
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  <img id="klammer_gross_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
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PROJECT
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  <img id="klammer_gross_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
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<div id="text-box2">
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    <div id="text_gross" align="left"><a href="https://2013.igem.org/Team:Freiburg/Project/1"> Our Project </a></div>
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  <div>
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<ul style="margin-left:40px;">
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<script src="https://ajax.googleapis.com/ajax/libs/jquery/1.6.2/jquery.min.js"></script>
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<li style="margin-top:-30px; padding-bottom:12px;"><a href="https://2013.igem.org/Team:Freiburg/Project/effector#activation">
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<script src="http://2012.igem-bielefeld.de/includes/wiki/flex/js/jquery.flexslider.js"></script>
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<span> Activation </span>
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<img  src="https://static.igem.org/mediawiki/2013/0/03/Freiburg_2013_main2_Activation.png" style="height:65px;">
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</li>
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</a>
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<li style="margin-left: -40px; padding-bottom:12px;"><a href="https://2013.igem.org/Team:Freiburg/Project/effector#repression">
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<span> Repression </span>
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<li style="margin-bottom:-30px;"><a href="https://2013.igem.org/Team:Freiburg/Project/effector#epigenetics">
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  });
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<span> Epigenetics </span>
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</script>
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<img src="https://static.igem.org/mediawiki/2013/3/31/Freiburg_2013_main2_Epigenetic.png">
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</li>
 +
</a>
 +
</ul>
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</head>
 
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<ul style="margin-left:160px">
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<!-- ******************************************************************* -->
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<li style="margin-top: -30px; padding-bottom:10px;"><a href="https://2013.igem.org/Team:Freiburg/Project/crrna">
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<!-- Body -->
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  <img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png">
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<!-- ******************************************************************* -->
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<span> Multiple targeting </span>
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</li>
 +
</a>
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<li style="margin-left: 40px; padding-bottom:8px;"><a href="https://2013.igem.org/Team:Freiburg/Project/method">
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  <img src="https://static.igem.org/mediawiki/2013/e/ef/UniBAss_freiburg_13.png" style="height:68px;">
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<span> uniBAss</span>
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</li>
 +
</a>
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<li style="margin-bottom:-30px;"><a href="https://2013.igem.org/Team:Freiburg/Project/induction#light">
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  <img src="https://static.igem.org/mediawiki/2013/9/9a/Freiburg_2013_main2_Licht.png" style="height:57px;">
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<span> Light induction </span>
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</li>
 +
</a>
 +
</ul>
 +
</div>
 +
</div>
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  <img id="klammer_gross_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
 +
</td>
 +
</tr>
 +
</table>
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<body>
 
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<!-- ########################################################################################
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<div id="main">
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TABLE UNTEN
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<a src="https://2013.igem.org/Team:Freiburg/Project/1"> PROJECT DESCRIPTION </a>
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######################################################################################## -->
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<div style="padding-top:5px; z-index:1;">
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        <div class="flexslider">
 
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            <ul class="slides">
 
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                  <li>
 
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                    <div class="frontpage-slide">
 
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                        <img src="https://static.igem.org/mediawiki/2013/d/d2/Project_kachel.png">
 
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                        <div class="slide-right-col">
 
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                            <div class="slide-heading">
 
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                                Our Toolbox
 
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                            </div>
 
-
                         
 
-
all what you need
 
-
                            <p><a href="https://2013.igem.org/Team:Freiburg/Project/1">
 
-
                            Click here to read more</a> about our project
 
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                            </p>
 
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                        </div>
 
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                    <div class="frontpage-slide">
 
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                        <img src="">
 
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                        <div class="slide-right-col">
 
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                            <div class="slide-heading">
 
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                              I do not know
 
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                            </div>
 
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<table id="table_viereck" style="margin-top:30px;">
-
                            <p><a href="https://2013.igem.org/Team:Freiburg/Project/1">
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                            Click here to read more </a> about our methods...
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                          </p>
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                        </div>
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                <li>
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                    <div class="frontpage-slide">
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                        <img src="https://static.igem.org/mediawiki/2013/d/d2/Project_kachel.png">
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                        <div class="slide-right-col">
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                            <div class="slide-heading">
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                                The Potential
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                            </div>
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-
+
-
   
+
-
                            <p><a href="http://">
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-
                            Click here to read more</a> </p>                     
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                        </div>
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<!-- ########################################################################################
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                        <div class=clear></div>
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                <li>
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                    <div class="frontpage-slide">
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                      <img src="https://static.igem.org/mediawiki/2013/d/d2/Project_kachel.png">
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                        <div class="slide-right-col">
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                            <div class="slide-heading">
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                                Light-Activation
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                            </div>
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                            <p><a href="https://2013.igem.org/Team:Freiburg/Project/1">
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HIGHLIGHTS
-
                            Click here to read more</a>
+
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                          </p>
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 +
######################################################################################## -->
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                        </div>
 
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<tr>
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                    </div>
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  <td width="300px">
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                </li>
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  <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
 +
  <div id="text-box3">  
 +
      <div id="text_gross" align="left"><a style="position:absolute; top:678px; left:60px; z-index:2" href="https://2013.igem.org/Team:Freiburg/Highlights"> Highlights </div> </a>
 +
  <p style="position:absolute; top:720px; left:60px" > All about our <br> project in one <br> slide show! </p>
 +
<img src="https://static.igem.org/mediawiki/2013/0/0e/Freiburg_2013_main2_Highlights5.png" height="190" style="position:absolute; top:614px; left:105px; z-index:1">
 +
  </div>  
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  <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
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  </td>
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            </ul>
 
-
        </div>
 
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TOOLKIT
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 +
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  <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
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  <div id="text-box4">
 +
 
 +
      <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/toolkit">Manual </a></div>
 +
 
 +
    Use our Toolkit <br> to regulate your <br> genes!
 +
    <img src="https://static.igem.org/mediawiki/2013/5/5d/Freiburg_2013_main2_Toolkitnew.png" height="90" style="position:absolute; top:715px; left:464px">
 +
  </div>
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  </div>
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  <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
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<div id="kachel_project">
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<img src="https://static.igem.org/mediawiki/2013/d/d2/Project_kachel.png">
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<div id="kachel">
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HUMAN PRACTICE
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<img src="https://static.igem.org/mediawiki/2013/9/9e/Tool_kachel_2.png">
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<img src="https://static.igem.org/mediawiki/2013/f/fb/Team_kachel.png">
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</div>
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######################################################################################## -->
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  <td width="250px">
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  <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
 +
  <div id="text-box5">
 +
 
 +
      <div id="text_gross" align="left"> <a style="position:absolute; top:678px; left:660px; z-index:2" href="https://2013.igem.org/Team:Freiburg/HumanPractice"> Human <br><br> Practice  </a> </div>
 +
 +
  <img src="https://static.igem.org/mediawiki/2013/e/ee/Freibrug_startseite_Humanpractice.png" height="150" style="position:absolute; top:650px; left:655px; z-index:1">
 +
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    </div>
 +
  <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
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TEAM
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  <td width="250px">
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  <img id="klammer_klein_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png">
 +
  <div id="text-box6">
 +
 
 +
<div id="text_gross" align="left"> <a style="position:absolute; top:678px; left:910px; z-index:2" href="https://2013.igem.org/Team:Freiburg/News"> News  </a></div>
 +
<p style="padding-top:10px;">
 +
Have a look at our achievements in Lyon & Boston!
 +
</p>
 +
<img src="https://static.igem.org/mediawiki/2013/5/56/Best-foundational-advance-2013-freiburg.png" height="130" style="position:absolute; top:600px; left:980px; z-index:1">
 +
  </div>
</div>
</div>
 +
  <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
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  </td>
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</table>
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</div>
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Latest revision as of 23:52, 9 April 2014


Abstract
Our Team developed a universal toolkit, termed uniCAS, that enables customizable gene regulation in mammalian cells. Therefore, we engineered the recently understood and highly promising CRISPR/Cas9 system.
The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences. Our toolkit comprises not only a standardized dCas9 protein, but also different effector domains for efficient gene activation or repression.
Our Project
Highlights

All about our
project in one
slide show!

Manual
Use our Toolkit
to regulate your
genes!
Human

Practice
News

Have a look at our achievements in Lyon & Boston!

Retrieved from "http://2013.igem.org/Team:Freiburg"