Team:TU-Eindhoven/FNRPromotor

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{{:Team:TU-Eindhoven/Template:Lead}}To tune the level of protein expression, we used decoy sites. Decoy sites are tandem repeats of DNA where transcriptional factor can bind to but don't promote gene expression. Therefore it competes with promoter sites and lowers the gene expression rate.
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{{:Team:TU-Eindhoven/Template:Lead}}To let the bacteria sense low oxygen concentrations, a FNR promotor was used in this research. The promotor that was chosen is not a default one, since it is a so called tandem promotor. That is, it contains two FNR binding sites. The kinetics of this promotor are therefore different than the promotor previously described in the [[Team:TU-Eindhoven/StochasticModel Decoy Sites model]]. Here the necessary adaptations of the Decoy Model to account for this special promotor will be described.{{:Team:TU-Eindhoven/Template:LeadEnd}}
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{{:Team:TU-Eindhoven/Template:Ref | id=LeeDecoy | author=Tek-Hyung Lee and Narendra Maheshri | title=A regulatory role for repeated decoy transcription factor binding sites in target gene expression | journal=Molecular Systems Biology | edition=8 | pages=576 | year=2012 }}{{:Team:TU-Eindhoven/Template:LeadEnd}}
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==References==
==References==

Revision as of 15:06, 8 September 2013

FNR Promotor

To let the bacteria sense low oxygen concentrations, a FNR promotor was used in this research. The promotor that was chosen is not a default one, since it is a so called tandem promotor. That is, it contains two FNR binding sites. The kinetics of this promotor are therefore different than the promotor previously described in the Team:TU-Eindhoven/StochasticModel Decoy Sites model. Here the necessary adaptations of the Decoy Model to account for this special promotor will be described.

References