Team:Freiburg/Project/1
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<p class="first_order"><a href="https://2013.igem.org/Team:Freiburg/Project/1"> Overview </a></p> | <p class="first_order"><a href="https://2013.igem.org/Team:Freiburg/Project/1"> Overview </a></p> | ||
- | <p class="second_order"> <a href="abstract"> Abstract </a> </p> | + | <p class="second_order"> <a href="#abstract"> Abstract </a> </p> |
- | <p class="second_order"> <a href="Introduction"> Introduction </a> </p> | + | <p class="second_order"> <a href="#Introduction"> Introduction </a> </p> |
- | <p class="second_order"> <a href="aim"> Aim </a> </p> | + | <p class="second_order"> <a href="#aim"> Aim </a> </p> |
<p class="first_order"><a href="https://2013.igem.org/Team:Freiburg/Project/effector"> Effector </a></p> | <p class="first_order"><a href="https://2013.igem.org/Team:Freiburg/Project/effector"> Effector </a></p> |
Revision as of 01:08, 9 September 2013
Abstract
Just imagine there was a tool that combined all these aspects of lab work. A tool, that was able to rule several genes at once; a tool that allowed highly specific gene modulation via stimulus induction; a tool that would be a new approach to gene regulation.
This year’s Freiburg iGEM Team uses the prokaryotic CRISPR/Cas system to enable multiple endogenous gene regulation with minimal effort. The regulation is based on a protein-RNA-DNA interaction. Customizable RNAs function as a guide for our protein in order to target specific DNA sequences. By fusing effector domains to this protein, we aim at developing a tool for multiple and inducible gene activation and repression. Despite the system's prokaryotic origin, gene target sequences are adjustable for various organisms, offering a broad application variety of our tool. Due to its great potential, the CRISPR/Cas system has become of increasing importance in current research and can be implemented in a number of novel and interesting applications, such as gene therapy or tissue engineering.
Introduction
Aim