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Team:Paris Saclay/Notebook/July/26
From 2013.igem.org
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| - | * Well 1 : | + | * Well 1 : 2µL of NirB+3microL H2O+1µL of 6X loading dye quantité 2µL d'ADN ???????? |
| - | * Well 2 : | + | * Well 2 : 2µL of NirB+1µL of 6X loading dye |
* Well 3 : 5µL of NarK+1µL of 6X loading dye | * Well 3 : 5µL of NarK+1µL of 6X loading dye | ||
* Well 4 : 5µL of NarK+1µL of 6X loading dye | * Well 4 : 5µL of NarK+1µL of 6X loading dye | ||
Revision as of 15:48, 13 September 2013
Notebook : July 26
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003
1 - Ligation of RBS-AmilCP and Term-PSB1C3
Xavier
Used quantities :
- RBS-AmilCP : 2µL
- Bba_B0015 ?????? digested by ... : 2.5µL
- Buffer : 1µL
- Ligase : 1µL
- H2O : 3.5µL
Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006
1 - Electrophoresis of PCR products : NarK, NarG, NirB
Abdou
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Expected sizes :
- NarK, NarG, NirB : ...
|
We lost all our PCR products. We will do PCR again using more concentrated DNA and oligos. |
2 - PCR of NarK, NarG, NirB
Abdou, Xavier
Used quantities : cf PCR précédente !!!!!!!!!!!!! ... ADN non dilué
PCR program : cf PCR précédente !!!!!!!!! SCHEMA !!!!!!!!!!!!!!
3 - Electrophoresis of PCR products : NarK, NarG, NirB
Abdou, Xavier
| [[]] |
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Expected sizes :
- NarK, NarG, NirB : ...
|
We obtain fragments at the right size. We can purify it. |
4 - Gel purification of PCR products : NarK, NarG, NirB
Xavier
Protocol : Gel purification
REPRIS dans 20µL !!!!!!!! ????????? PAS DE NANODROP ??????
