Revision as of 14:14, 17 September 2013

Notebook : August 20

Damir

Used quantities :

PCR Program :

Nadia

[[]]	Well 1 : 6µL DNA Ladder Well 2 : 40µL BphR2 PartI+8µl of 6X loading dye Gel : 0.8%

We obtain a frangment at the right size. We can purify it.

Damir, Nadia

Nanodrop :

CONCLUSION ON A TOUS PERDU D'OU ON RECOMMENCE ENSUITE ??????

@@ Line 5: / Line 5: @@
 =='''Lab work'''==
-===''''''===
+==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===
-====''''''====
+===='''Objective : obtaining FNR and BphR2 proteins'''====
-===='''1 - '''====
+===='''1 - PCR of BphR2 Part I'''====
+Damir
+Used quantities :
+*
+PCR Program :
+===='''2 - Electrophoresis of PCR products :  BphR2 Part I'''====
+Nadia
+{|
+| style="width:250px;border:1px solid black;" | [[]]
+| style="width:350px;border:1px solid black;vertical-align:top;" |
+*Well 1 : 6µL DNA Ladder
+*Well 2 : 40µL BphR2 PartI+8µl of 6X loading dye
+*Gel : 0.8%
+|}
+{|
+| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
+We obtain a frangment at the right size. We can purify it.
+|}
+====2 - Gel purification of PCR products : BphR2 Part I ====
+Damir, Nadia
+Protocol : [[Team:Paris_Saclay/Protocols/Gel purification|Gel purification]]
+Nanodrop :
+* BphR2 Part I: .........
+ {|
+| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
+CONCLUSION ON A TOUS PERDU D'OU ON RECOMMENCE ENSUITE ??????
+|}
 {{Team:Paris_Saclay/incl_fin}}