Team:TU-Delft/Planning
From 2013.igem.org
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Project 'Peptidor' deals with various cloning schemes into <i>E.coli</i> cells. They are made to sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and then start producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>. Several bio-bricks from the parts registry were used to clone the <i>E. coli</i>. There are many test constructs made in order to test and characterize each module. A detailed cloning strategy is as follows: | Project 'Peptidor' deals with various cloning schemes into <i>E.coli</i> cells. They are made to sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and then start producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>. Several bio-bricks from the parts registry were used to clone the <i>E. coli</i>. There are many test constructs made in order to test and characterize each module. A detailed cloning strategy is as follows: | ||
</p> | </p> | ||
+ | <h4>His 6 - SUMO Peptide construct </h4> <center> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/4/47/His6-SUMOPeptide-construct.jpg" height=950px width=680px ></center> | ||
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+ | <h4>AIP test Construct </h4><center> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/d/d4/AIP-test-constructs.jpg" height=950px width=700px ></center> | ||
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Revision as of 22:07, 15 September 2013
Planning
Project 'Peptidor' deals with various cloning schemes into E.coli cells. They are made to sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and then start producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Several bio-bricks from the parts registry were used to clone the E. coli. There are many test constructs made in order to test and characterize each module. A detailed cloning strategy is as follows: