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Team:Paris Saclay/Notebook/August/5
From 2013.igem.org
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| Line 54: | Line 54: | ||
Used quantities : | Used quantities : | ||
* DNA : 2µL | * DNA : 2µL | ||
| - | * Mix : (it was divided in 25 tubes | + | * Mix : (it was divided in 25 tubes for each promotor with 23µL of mix in each on) |
** Oligo 44 : 3.5µL | ** Oligo 44 : 3.5µL | ||
** Oligo 45 : 3.5µL | ** Oligo 45 : 3.5µL | ||
Revision as of 15:32, 16 September 2013
Notebook : August 5
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003
1 - Digestion of Bba_K1155003 by EcoRI/PstI
Nadia, XiaoJing
Used quantities :
- Bba_K1155003 : 5µL
- EcoRI FD : 1µL
- PstI FD : 1µL
- Buffer FD : 3µL
- H2O : 20µL
We let the digestion at 37°C during 10 minutes ??????
2 - Electrophoresis of the digestion of Bba_K1155003
Damir
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Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006
Damir, Nadia, XiaoJing
1 - Colony PCR of Bba_K1155004, Bba_K115605, Bba_K1155006 in DH5α
Damir, Nadia, XiaoJing
OU EST LA TRANFO PRECEDENTE ????????!!!!!!!
COLONIES PIQUEES DANS 10µL d'eau par Tube !!!!!!!!!!!!!
Used quantities :
- DNA : 2µL
- Mix : (it was divided in 25 tubes for each promotor with 23µL of mix in each on)
- Oligo 44 : 3.5µL
- Oligo 45 : 3.5µL
- Buffer Dream Taq : 70µL
- dNTP : 28µL
- Dream Taq : 5µL
- H2O : 590µL
PCR Program :
