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Team:TU-Delft/PeptideProduction
From 2013.igem.org
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Project 'Peptidor' deals with various cloning schemes into <i>E.coli</i> cells. They are made to sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and then start producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>. Several bio-bricks from the parts registry were used to clone the <i>E. coli</i>. There are many test constructs made in order to test and characterize each module. Both the detailed cloning strategies related to each module and the final constructs can be viewed in the following links: | Project 'Peptidor' deals with various cloning schemes into <i>E.coli</i> cells. They are made to sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and then start producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>. Several bio-bricks from the parts registry were used to clone the <i>E. coli</i>. There are many test constructs made in order to test and characterize each module. Both the detailed cloning strategies related to each module and the final constructs can be viewed in the following links: | ||
<ul style="list-style-type: circle"> | <ul style="list-style-type: circle"> | ||
| - | <li><a href="https://2013.igem.org/Team:TU-Delft/PeptideProduction" style="text-decoration: none | + | <li><a href="https://2013.igem.org/Team:TU-Delft/PeptideProduction" style="text-decoration: none"><font color="#0080FF" size="3">Peptide Production Cloning Schemes</font></a></li> |
| - | <li><a href="https://2013.igem.org/Team:TU-Delft/KillSwitchPlanning" style="text-decoration: none | + | <li><a href="https://2013.igem.org/Team:TU-Delft/KillSwitchPlanning" style="text-decoration: none"><font color="#0080FF" size="3">Kill Switch Cloning Schemes</font></a></li> |
| - | <li><a href="https://2013.igem.org/Team:TU-Delft/Sensing Device" style="text-decoration: none | + | <li><a href="https://2013.igem.org/Team:TU-Delft/Sensing Device" style="text-decoration: none"><font color="#0080FF" size="3">Sensing Device Cloning Schemes</font></a></li> |
| - | <li><a href="https://2013.igem.org/Team:TU-Delft/FinalConstructs" style="text-decoration: none | + | <li><a href="https://2013.igem.org/Team:TU-Delft/FinalConstructs" style="text-decoration: none"><font color="#0080FF" size="3">Final Constructs</font></a></li> |
| - | <li><a href="https://2013.igem.org/Team:TU-Delft/All" style="text-decoration: none | + | <li><a href="https://2013.igem.org/Team:TU-Delft/All" style="text-decoration: none"><font color="#0080FF" size="3">All Cloning Schemes</font></a> </li> |
</ul> | </ul> | ||
Revision as of 15:02, 18 September 2013
Planning
Project 'Peptidor' deals with various cloning schemes into E.coli cells. They are made to sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and then start producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Several bio-bricks from the parts registry were used to clone the E. coli. There are many test constructs made in order to test and characterize each module. Both the detailed cloning strategies related to each module and the final constructs can be viewed in the following links:
- Peptide Production Cloning Schemes
- Kill Switch Cloning Schemes
- Sensing Device Cloning Schemes
- Final Constructs
- All Cloning Schemes
