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Team:Freiburg/Project/toolkit
From 2013.igem.org
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Now that you have created the desired crRNA plasmids it is possible to fuse all crRNA loci together into one crRNA plasmid (recommended). If you want to use them individually just skip this part. | Now that you have created the desired crRNA plasmids it is possible to fuse all crRNA loci together into one crRNA plasmid (recommended). If you want to use them individually just skip this part. | ||
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Experimental design (recommendation for a mammalian cell culture): | Experimental design (recommendation for a mammalian cell culture): | ||
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<li> Transfect BBa_K1150020 without any crRNA plasmid as off target control </li> | <li> Transfect BBa_K1150020 without any crRNA plasmid as off target control </li> | ||
<li> Transfect BBa_K1150020 with all desired crRNA plasmids (seperate and in combinitions) </li> | <li> Transfect BBa_K1150020 with all desired crRNA plasmids (seperate and in combinitions) </li> | ||
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<img src="https://static.igem.org/mediawiki/2013/6/62/Toolbox_back_bottom_freiburg_13.png" style="width:850px;"> | <img src="https://static.igem.org/mediawiki/2013/6/62/Toolbox_back_bottom_freiburg_13.png" style="width:850px;"> | ||
Revision as of 10:02, 20 September 2013
The uniCAS toolkit - Customize your experiments!
You want to have a maximum of activation or repression of your genes by a minimal effort? Then you have to use the uniCAS toolkit provided by the iGEM team Freiburg 2013. All you have to do is:
- Click yourself through the routine below
- Order the appropriate plasmids and oligos
- Conduct a minimal of cloning
- Start your personalized experiment
